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    STREPTAVIDIN-COATED SOLID PHASES WITH A MEMBER OF A BINDING PAIR
    2.
    发明申请
    STREPTAVIDIN-COATED SOLID PHASES WITH A MEMBER OF A BINDING PAIR 有权

    公开(公告)号：US20220011301A1

    公开(公告)日：2022-01-13

    申请号：US17292660

    申请日：2019-11-14

    Applicant: Roche Diagnostics Operations, Inc.

    Inventor： Frank BERGMANN ,  Dieter HEINDL ,  Tobias OELSCHLAEGEL ,  Johannes STOECKEL

    IPC: G01N33/543

    Abstract: The present disclosure relates to a solid phase coated with (strept)avidin and having attached thereto, by way of biotin:(strept)avidin interaction, a biotinylated first member of a binding pair, wherein the attached first member is capable of binding to a second member of the binding pair, but is not capable of binding to biotin or to (strept)avidin, and wherein no member of the binding pair is capable of hybridizing with a naturally-occurring single-stranded nucleic acid. The solid phase is particularly useful in immunoassays with samples having high content in biotin or (strept)avidin-binding derivatives thereof. The present disclosure further provides uses, kits and methods, particularly for determination of an analyte in a sample.

    METHODS AND COMPOSITIONS FOR DETECTION AND ANALYSIS OF ANALYTES
    3.
    发明申请
    METHODS AND COMPOSITIONS FOR DETECTION AND ANALYSIS OF ANALYTES 有权

    公开(公告)号：US20210088511A1

    公开(公告)日：2021-03-25

    申请号：US17039245

    申请日：2020-09-30

    Applicant: Roche Diagnostics Operations, Inc.

    Inventor： Peter CRISALLI ,  Dmitriy GREMYACHINSKIY ,  Dieter HEINDL ,  Hannes KUCHELMEISTER ,  Michael SCHRAEML ,  Andrew TRANS

    IPC: G01N33/543 ,  G01N33/487

    Abstract: Provided are nanopore-based methods, compositions, and systems for assessing analyte-ligand interactions and analyte concentration in a fluid solution. The compositions include an analyte detection complex that is associated with a nanopore to form a nanopore assembly, the analyte detection complex including an analyte ligand. As a first voltage is applied across the nanopore assembly, the analyte ligand is presented to an analyte in the solution. As a second voltage that is opposite in polarity to the first voltage is applied across the nanopore assembly, the analyte binds to the analyte. By comparing the total number of analyte-ligand binding pairs to a control binding count, the concentration of the analyte can be determined. In other examples, further increasing the second voltage can result in dissociation of the analyte-ligand pair, from which a dissociation voltage—and hence a dissociation constant—can be determined.

    METHODS FOR DETECTING AN ANALYTE USING STRUCTURE SWITCHING BINDING AGENTS
    5.
    发明申请
    METHODS FOR DETECTING AN ANALYTE USING STRUCTURE SWITCHING BINDING AGENTS 有权

    公开(公告)号：US20220404347A1

    公开(公告)日：2022-12-22

    申请号：US17350822

    申请日：2021-06-17

    Applicant: Roche Diagnostics Operations, Inc. ,  Roche Sequencing Solutions, Inc.

    Inventor： Yann ASTIER ,  Dieter HEINDL ,  Hannes KUCHELMEISTER ,  Nikolaus-Peter STENGELE

    IPC: G01N33/543 ,  C12N15/115 ,  C12N15/10

    Abstract: The present invention relates to diagnostic test and technology. In particular, it relates to a method for determining an analyte suspected to be present in a sample comprising contacting said sample with at least one sensor element comprising at least one binding agent which is capable of specifically binding to the analyte and which comprises at least one magnetic label; and in functional proximity thereto a magnetic tunnel junction generating a signal which is altered upon binding of the analyte to the binding agent for a time and under conditions which allow for specific binding of the analyte suspected to be present in the sample to the at least one binding agent, measuring an altered signal generated by the magnetic tunnel junction upon analyte binding to the at least one binding agent comprising the at least one magnetic label, and determining the analyte based on the altered signal which is generated by the magnetic tunnel junction. The present invention further relates to a device for determining an analyte suspected to be present in a sample and for using such a device. Moreover, the present invention furthermore relates to an aptamer which is capable of specifically binding to an analyte and which comprises at least one magnetic label and a method for identifying such an aptamer. Finally, the invention relates to a kit for determining an analyte suspected to be present in a sample.

    PARTICLE-BASED IMMUNOASSAY USING A PEGYLATED ANALYTE-SPECIFIC BINDING AGENT
    6.
    发明申请
    PARTICLE-BASED IMMUNOASSAY USING A PEGYLATED ANALYTE-SPECIFIC BINDING AGENT 有权

    公开(公告)号：US20220317119A1

    公开(公告)日：2022-10-06

    申请号：US17838568

    申请日：2022-06-13

    Applicant: Roche Diagnostics Operations, Inc.

    Inventor： Toralf ZARNT ,  Dieter HEINDL ,  Alfons NICHTL ,  Frank SICHERL-BIRK

    IPC: G01N33/543

    Abstract: Disclosed is a method for measurement of an analyte in a microparticle-based analyte-specific binding assay, wherein the microparticles are coated with the first partner of a binding pair, the method involving mixing the coated microparticles, an analyte-specific binding agent conjugated to the second partner of the binding pair, and a sample suspected of containing or containing the analyte, wherein the second partner of the binding pair is bound to the analyte-specific binding agent via a linker having from 12 to 30 ethylene glycol units (PEG 12 to 30), thereby binding the analyte via the conjugated analyte-specific binding agent to the coated microparticles, separating the microparticles having the analyte bound via the binding pair and the analyte-specific binding agent from the mixture and measuring the analyte bound to the microparticles.

    HYBRIDIZING all-LNA OLIGONUCLEOTIDES
    7.
    发明申请
    HYBRIDIZING all-LNA OLIGONUCLEOTIDES 有权

    公开(公告)号：US20220195497A1

    公开(公告)日：2022-06-23

    申请号：US17544110

    申请日：2021-12-07

    Applicant: Roche Diagnostics Operations, Inc.

    Inventor： Frank BERGMANN ,  Dieter HEINDL ,  Michael SCHRAEML ,  JOHANNES STOECKEL

    IPC: C12Q1/6816

    Abstract: The present report relates to hybridizing single-stranded (ss-) oligonucleotides which entirely consist of locked nucleic acid (LNA) monomers. The present document shows hybridization experiments with pairs of entirely complementary ss-oligonucleotides which fail to form a duplex within a given time interval. The present report provides methods to identify such incompatible oligonucleotide pairs. In another aspect, the present report provides pairs of complementary ss-oligonucleotides which are capable of rapid duplex formation. The present report also provides methods to identify and select compatible oligonucleotide pairs. In yet another aspect the present report provides use of compatible oligonucleotide pairs as binding partners in binding assays, e.g. receptor-based assays.

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