公开(公告)号：US20160324144A1

公开(公告)日：2016-11-10

申请号：US15221791

申请日：2016-07-28

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Waltraud Ankenbauer ,  Ulrike Dietrich-Veenstra ,  Renate Kolb ,  Yvonne Maerz ,  Stephanie Wessner

IPC: A01N1/02

CPC classification number: A01N1/0226

Abstract: The present disclosure is directed to the stabilization of nucleated cells in a whole blood sample ex-vivo, effected by an additive being a liquid composition for stabilizing an analyte in intact nucleated cells of a whole-blood sample ex-vivo, the composition being an aqueous solution, the solution comprising an anticoagulant, a phosphate salt, a cell-metabolizable sugar, adenine, and an antioxidant, wherein the antioxidant comprises a mitochondria-targeted antioxidant, preferably a mitochondria-targeted antioxidant selected from the group consisting of SkQ1, MitoQ, SS-31, and a mixture thereof. In a specific embodiment, the liquid composition further comprises a protease inhibitor. Further provided is advantageous use of the composition for stabilizing an analyte selected from DNA, RNA and protein in intact nucleated cells of the whole-blood sample ex-vivo, as well as kits including the composition for practicing said use. Additionally, specific methods are provided for stabilizing intact nucleated cells of a whole-blood sample ex-vivo.

Abstract translation: 本公开涉及离体全血样品中的有核细胞的稳定化，其通过添加剂进行稳定，所述添加剂是用于稳定全血样品的完整有核细胞中的分析物的液体组合物，该组合物为 所述溶液包含抗凝血剂，磷酸盐，细胞代谢糖，腺嘌呤和抗氧化剂，其中所述抗氧化剂包含线粒体靶向的抗氧化剂，优选线粒体靶向的抗氧化剂，其选自SkQ1，MitoQ ，SS-31及其混合物。 在具体实施方案中，液体组合物还包含蛋白酶抑制剂。 进一步提供了组合物有利地用于稳定离体全血样品的完整有核细胞中选自DNA，RNA和蛋白质的分析物，以及包括用于实践所述用途的组合物的试剂盒。 另外，提供了用于稳定全血样品的完整有核细胞的具体方法。

公开(公告)号：US20160252498A1

公开(公告)日：2016-09-01

申请号：US15146273

申请日：2016-05-04

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Waltraud Ankenbauer ,  Thomas Froehlich ,  Stefanie Froehner

IPC: G01N33/50

CPC classification number: G01N33/5094 ,  G01N33/574

Abstract: The present disclosure relates to use of a reagent for the lysis of erythrocytes, a method of lysing erythrocytes and a kit comprising the reagent.

Abstract translation: 本公开内容涉及用于红细胞裂解的试剂的使用，红细胞的裂解方法和包含该试剂的试剂盒。