公开(公告)号：US09506918B2

公开(公告)日：2016-11-29

申请号：US14673049

申请日：2015-03-30

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Thomas Fischer ,  Thomas Froehlich ,  Dieter Hendl

IPC: G01N33/543 ,  G01N33/50 ,  C12Q1/68

CPC classification number: G01N33/54306 ,  C12Q1/6823 ,  C12Q1/6834 ,  G01N33/5005 ,  G01N2800/00 ,  C12Q2521/301 ,  C12Q2525/131 ,  C12Q2565/518

Abstract: The present invention relates to a method of specifically releasing a one or more members of a sub-group of objects from an entity, a method of detecting a subject's disease by detecting one or more members of a sub-group of biological entities indicative of the disease and a method of isolating one or more members of a sub-group of objects from a group of objects.

Abstract translation: 本发明涉及一种从实体特异性地释放物体子组的一个或多个成员的方法，通过检测生物实体子组的一个或多个成员来检测受试者的疾病的方法， 疾病和从一组对象中分离对象子组的一个或多个成员的方法。

公开(公告)号：US20210102944A1

公开(公告)日：2021-04-08

申请号：US17115069

申请日：2020-12-08

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Hans-Peter Josel ,  Dieter Heindl ,  Thomas Froehlich ,  Stefanie Froehner

IPC: G01N33/569 ,  C12N11/06 ,  G01N33/50 ,  G01N33/543 ,  C12N5/00 ,  C12N11/14 ,  C12N11/08

Abstract: The present invention relates to a method of immobilizing a cell on a support, the method comprising a) providing a compound or salt thereof comprising, preferably consisting of, one or more hydrophobic domains attached to a hydrophilic domain, wherein the one or more hydrophobic domains are covalently bound to said hydrophilic domain, and wherein the one or more hydrophobic domains each comprise a linear lipid, a steroid or a hydrophobic vitamin, and wherein the hydrophilic domain comprises a polyethylene glycol (PEG) moiety, and wherein the compound comprises a linking group; b) contacting a cell with the compound under conditions allowing the interaction of the compound with the membrane of the cell, thereby immobilizing the linking group on the surface of the cell; and c) contacting the linking group immobilized on the cell with a support capable of binding the linking group, thereby immobilizing the cell on the support.

公开(公告)号：US10890585B2

公开(公告)日：2021-01-12

申请号：US15186581

申请日：2016-06-20

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Hans-Peter Josel ,  Dieter Heindl ,  Thomas Froehlich ,  Stefanie Froehner

IPC: C12N11/06 ,  C12N11/08 ,  G01N33/569 ,  G01N33/50 ,  G01N33/543 ,  C12N5/00 ,  C12N11/14

Abstract: The present invention relates to a method of immobilizing a cell on a support, the method comprising a) providing a compound or salt thereof comprising, preferably consisting of, one or more hydrophobic domains attached to a hydrophilic domain, wherein the one or more hydrophobic domains are covalently bound to said hydrophilic domain, and wherein the one or more hydrophobic domains each comprise a linear lipid, a steroid or a hydrophobic vitamin, and wherein the hydrophilic domain comprises a polyethylene glycol (PEG) moiety, and wherein the compound comprises a linking group; b) contacting a cell with the compound under conditions allowing the interaction of the compound with the membrane of the cell, thereby immobilizing the linking group on the surface of the cell; and c) contacting the linking group immobilized on the cell with a support capable of binding the linking group, thereby immobilizing the cell on the support.

公开(公告)号：US20170363624A1

公开(公告)日：2017-12-21

申请号：US15186581

申请日：2016-06-20

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Hans-Peter Josel ,  Dieter Heindl ,  Thomas Froehlich ,  Stefanie Froehner

IPC: G01N33/569 ,  C12N11/08 ,  C12N11/06

CPC classification number: G01N33/56972 ,  C12N5/0068 ,  C12N11/06 ,  C12N11/08 ,  C12N11/14 ,  C12N2503/00 ,  C12N2533/20 ,  C12N2533/30 ,  G01N33/5005 ,  G01N33/54353 ,  G01N33/56966

Abstract: The present invention relates to a method of immobilizing a cell on a support, the method comprising a) providing a compound or salt thereof comprising, preferably consisting of, one or more hydrophobic domains attached to a hydrophilic domain, wherein the one or more hydrophobic domains are covalently bound to said hydrophilic domain, and wherein the one or more hydrophobic domains each comprise a linear lipid, a steroid or a hydrophobic vitamin, and wherein the hydrophilic domain comprises a polyethylene glycol (PEG) moiety, and wherein the compound comprises a linking group; b) contacting a cell with the compound under conditions allowing the interaction of the compound with the membrane of the cell, thereby immobilizing the linking group on the surface of the cell; and c) contacting the linking group immobilized on the cell with a support capable of binding the linking group, thereby immobilizing the cell on the support.

公开(公告)号：US10718769B2

公开(公告)日：2020-07-21

申请号：US15186578

申请日：2016-06-20

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Hans-Peter Josel ,  Dieter Heindl ,  Thomas Froehlich ,  Stefanie Froehner

IPC: C07J17/00 ,  C07J9/00 ,  G01N33/58 ,  C07D311/04 ,  C07D311/82 ,  G01N33/569 ,  C07J51/00 ,  C12N11/06 ,  G01N33/50 ,  G01N33/543 ,  C07D495/04

Abstract: The present invention relates to novel compounds comprising one or more hydrophobic domains and a hydrophilic domain comprising PEG moieties, useful for binding cells, as well as uses and compositions related thereto. The compounds are useful for immobilizing and/or stabilizing cells.

公开(公告)号：US11021472B2

公开(公告)日：2021-06-01

申请号：US15186580

申请日：2016-06-20

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Hans-Peter Josel ,  Dieter Heindl ,  Thomas Froehlich ,  Stefanie Froehner

IPC: C07D409/04 ,  C12M1/42 ,  C07J51/00 ,  G01N33/543 ,  G01N33/50 ,  C12N5/00

Abstract: The present invention relates to the use compounds comprising two or more hydrophobic domains and a hydrophilic domain comprising a polyethylene glycol (PEG) moiety for stabilization of a cell, and methods related thereto.

公开(公告)号：US20160252498A1

公开(公告)日：2016-09-01

申请号：US15146273

申请日：2016-05-04

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Waltraud Ankenbauer ,  Thomas Froehlich ,  Stefanie Froehner

IPC: G01N33/50

CPC classification number: G01N33/5094 ,  G01N33/574

Abstract: The present disclosure relates to use of a reagent for the lysis of erythrocytes, a method of lysing erythrocytes and a kit comprising the reagent.

Abstract translation: 本公开内容涉及用于红细胞裂解的试剂的使用，红细胞的裂解方法和包含该试剂的试剂盒。

公开(公告)号：US20150204863A1

公开(公告)日：2015-07-23

申请号：US14673049

申请日：2015-03-30

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Thomas Fischer ,  Thomas Froehlich ,  Dieter Hendl

IPC: G01N33/543

CPC classification number: G01N33/54306 ,  C12Q1/6823 ,  C12Q1/6834 ,  G01N33/5005 ,  G01N2800/00 ,  C12Q2521/301 ,  C12Q2525/131 ,  C12Q2565/518

Abstract: The present invention relates to a method of specifically releasing a one or more members of a sub-group of objects from an entity, a method of detecting a subject's disease by detecting one or more members of a sub-group of biological entities indicative of the disease and a method of isolating one or more members of a sub-group of objects from a group of objects.

Abstract translation: 本发明涉及一种从实体特异性地释放物体子组的一个或多个成员的方法，通过检测生物实体子组的一个或多个成员来检测受试者的疾病的方法， 疾病和从一组对象中分离对象子组的一个或多个成员的方法。

公开(公告)号：US11965886B2

公开(公告)日：2024-04-23

申请号：US17115069

申请日：2020-12-08

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Hans-Peter Josel ,  Dieter Heindl ,  Thomas Froehlich ,  Stefanie Froehner

IPC: G01N33/50 ,  C12N5/00 ,  C12N11/06 ,  C12N11/089 ,  C12N11/14 ,  G01N33/543 ,  G01N33/554 ,  G01N33/569

CPC classification number: G01N33/56972 ,  C12N5/0068 ,  C12N11/06 ,  C12N11/089 ,  C12N11/14 ,  G01N33/5005 ,  G01N33/54353 ,  G01N33/56966 ,  C12N2503/00 ,  C12N2533/20 ,  C12N2533/30

Abstract: The present invention relates to a method of immobilizing a cell on a support, the method comprising a) providing a compound or salt thereof comprising, preferably consisting of, one or more hydrophobic domains attached to a hydrophilic domain, wherein the one or more hydrophobic domains are covalently bound to said hydrophilic domain, and wherein the one or more hydrophobic domains each comprise a linear lipid, a steroid or a hydrophobic vitamin, and wherein the hydrophilic domain comprises a polyethylene glycol (PEG) moiety, and wherein the compound comprises a linking group; b) contacting a cell with the compound under conditions allowing the interaction of the compound with the membrane of the cell, thereby immobilizing the linking group on the surface of the cell; and c) contacting the linking group immobilized on the cell with a support capable of binding the linking group, thereby immobilizing the cell on the support.

公开(公告)号：US20180141935A1

公开(公告)日：2018-05-24

申请号：US15186580

申请日：2016-06-20

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Hans-Peter Josel ,  Dieter Heindl ,  Thomas Froehlich ,  Stefanie Froehner

IPC: C07D409/04 ,  C12M1/42

CPC classification number: C07D409/04 ,  C07J51/00 ,  C12M35/04 ,  C12N5/0068 ,  C12N2533/20 ,  C12N2533/30 ,  G01N33/5005 ,  G01N33/54353

Abstract: The present invention relates to the use compounds comprising two or more hydrophobic domains and a hydrophilic domain comprising a polyethylene glycol (PEG) moiety for stabilization of a cell, and methods related thereto.