公开(公告)号：US20190381181A1

公开(公告)日：2019-12-19

申请号：US16008281

申请日：2018-06-14

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Michael Schraeml ,  Wojtek Steffen

IPC: A61K47/65 ,  C07K1/13 ,  C12N9/90

Abstract: The present disclosure relates to a recombinant transglutaminase (TG) substrate having an amino acid sequence of the FKBP domain of an FKBP polypeptide, wherein the “insert-in-flap” (IF) domain thereof is, at least in part, replaced by an amino acid sequence (“Q-tag”) of 5 to 20 amino acids with a sequence having at least 80% sequence identity to the YRYRQ portion of the peptide sequence X1-YRYRQ-X2 (SEQ ID NO. 1), and wherein said TG substrate is a substrate for the TG function of the Kutzneria albida TG. The present disclosure furthermore relates to uses of said substrate.

公开(公告)号：US20240140995A1

公开(公告)日：2024-05-02

申请号：US18542054

申请日：2023-12-15

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Romina Eisenhauer ,  Alfred Engel ,  Ute Jucknischke ,  Michael Schraeml ,  Wojtek Steffen

IPC: C07K14/195 ,  G01N33/543

CPC classification number: C07K14/195 ,  G01N33/54353 ,  C07K2319/00 ,  G01N2333/245

Abstract: The present invention relates to a method of determining an analyte in a sample, said method comprising (a) contacting said sample with (i) a binding compound binding to said analyte, said binding compound comprising a binding agent and a first partner of an affinity pair (first affinity partner); and (ii) a second partner of the affinity pair (second affinity partner) coupled to a solid surface, to an indicator, and/or to a second binding agent; and (b) determining said analyte based on complexes formed in step (a); wherein one of said first affinity partner and said second affinity partner is a polypeptide comprising the amino acid sequence of SEQ ID NO:1 or a sequence at least 50% identical thereto, and wherein the other of said first affinity partner and said second affinity partner is a polypeptide comprising the amino acid sequence of SEQ ID NO:2 or a sequence at least 50% identical thereto. The present invention also relates to a polypeptide comprising an amino acid sequence as specified in SEQ ID NO:1 or a sequence at least 50% identical thereto, wherein the amino acid at the position corresponding to position 77 in SEQ ID NO:1 is not a histidine; and to a polypeptide comprising an amino acid sequence as specified in SEQ ID NO:2 or a sequence at least 50% identical thereto, wherein (i) the amino acid at the position corresponding to position 17 in SEQ ID NO:2 is not a cysteine, in an embodiment is alanine, serine, leucine, isoleucine, or glycine and/or (ii) said polypeptide further comprises at least one functional peptide; and to fusion polypeptides, polypeptide complexes, polynucleotides and kit related to the aforesaid.