公开(公告)号：US11293060B2

公开(公告)日：2022-04-05

申请号：US16596539

申请日：2019-10-08

申请人： Roche Sequencing Solutions, Inc.

发明人： Frank Bergmann ,  Ian Quang Chau ,  Peter Crisalli ,  Hannah Marie Kallewaard-Lum ,  Hannes Kuchelmeister ,  Sebastian Johannes Pomplun ,  Hasham Saqib ,  Christian Wellner

IPC分类号： C12Q1/68 ,  C12Q1/6869 ,  C07D295/13 ,  C07D487/04 ,  C07H21/02 ,  C07H21/04 ,  A61K47/68

摘要： The present invention relates to improving the processing rate of a sequencing reaction, for example in a nanopore sequencing reaction, by means of using improved nucleoside-tags. The tags are linked to the nucleoside phosphate via a Pictet Spengler reaction. Exemplary sequencing reactions that are improved by the present methods include nanopore-based nucleic acid sequencing-by-synthesis reactions.

公开(公告)号：US20200040392A1

公开(公告)日：2020-02-06

申请号：US16596539

申请日：2019-10-08

申请人： Roche Sequencing Solutions, Inc.

发明人： Frank Bergmann ,  Ian Quang Chau ,  Peter Crisalli ,  Hannah Marie Kallewaard-Lum ,  Hannes Kuchelmeister ,  Sebastian Johannes Pomplun ,  Hasham Saqib ,  Christian Wellner

IPC分类号： C12Q1/6869

摘要： The present invention relates to improving the processing rate of a sequencing reaction, for example in a nanopore sequencing reaction, by means of using improved nucleoside-tags. The tags are linked to the nucleoside phosphate via a Pictet Spengler reaction. Exemplary sequencing reactions that are improved by the present methods include nanopore-based nucleic acid sequencing-by-synthesis reactions.