公开(公告)号：US20240010668A1

公开(公告)日：2024-01-11

申请号：US18233265

申请日：2023-08-11

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Frank Bergmann ,  Peter Crisalli ,  Dieter Heindl ,  Omid Khakshoor ,  Meng Taing

IPC: C07H19/06 ,  C07H19/048

CPC classification number: C07H19/06 ,  C07H19/048

Abstract: The present disclosure provides 3′ protected nucleotides, including those 3′ protected nucleotides having a detectable tag. Systems and methods of sequencing nucleic acids using the 3′ protected nucleotides are also disclosed, such as the sequencing of a nucleic acid using a nanopore or the sequencing of a nucleic acid via sequencing-by-synthesis.

公开(公告)号：US11773130B2

公开(公告)日：2023-10-03

申请号：US17858099

申请日：2022-07-06

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Frank Bergmann ,  Peter Crisalli ,  Dieter Heindl ,  Omid Khakshoor ,  Meng Taing

IPC: C07H19/06 ,  C07H19/048

CPC classification number: C07H19/06 ,  C07H19/048

Abstract: The present disclosure provides 3′ protected nucleotides, including those 3′ protected nucleotides having a detectable tag. Systems and methods of sequencing nucleic acids using the 3′ protected nucleotides are also disclosed, such as the sequencing of a nucleic acid using a nanopore or the sequencing of a nucleic acid via sequencing-by-synthesis.

公开(公告)号：US20230159999A1

公开(公告)日：2023-05-25

申请号：US17817480

申请日：2022-08-04

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Aruna Ayer ,  David Beyer ,  Peter Crisalli ,  Kirti Dhiman ,  Helen Franklin ,  Swathi Chakravarthy ,  Omid Khakshoor ,  Blake Langdon ,  Meng Taing ,  Adolfo Vargas

IPC: C12Q1/6869

CPC classification number: C12Q1/6869

Abstract: This application discloses compositions comprising primer compounds that reduce or block deleterious threading into a nanopore of nucleic acid strands displaced by a nanopore-linked polymerase, for example during the use of a nanopore device for nucleic acid sequencing. Also disclosed are methods for using the compositions to reduce deleterious threading events during nanopore-based nucleic acid detection techniques, such as nanopore sequencing.

公开(公告)号：US20220348604A1

公开(公告)日：2022-11-03

申请号：US17858099

申请日：2022-07-06

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Frank Bergmann ,  Peter Crisalli ,  Dieter Hiendl ,  Omid Khakshoor ,  Meng Taing

IPC: C07H19/06 ,  C07H19/048

Abstract: The present disclosure provides 3′ protected nucleotides, including those 3′ protected nucleotides having a detectable tag. Systems and methods of sequencing nucleic acids using the 3′ protected nucleotides are also disclosed, such as the sequencing of a nucleic acid using a nanopore or the sequencing of a nucleic acid via sequencing-by-synthesis.

公开(公告)号：US11525124B2

公开(公告)日：2022-12-13

申请号：US16871732

申请日：2020-05-11

Applicant: ROCHE SEQUENCING SOLUTIONS, INC.

Inventor： Helen Franklin ,  Cynthia Cech ,  Timothy Kellogg Craig ,  Aruna Ayer ,  Kirti Dhiman ,  Natalie B. Chechelski Johnston ,  Joshua N. Mabry ,  Arkadiusz Bibillo ,  Peter Crisalli ,  Randall W. Davis

IPC: C12N9/12 ,  C12Q1/6869 ,  B01L9/00 ,  C12N9/96 ,  C12Q1/6806 ,  C12Q1/6874

Abstract: Disclosed are methods for isolating polymerase complexes from a mixture of polymerase complex components. The polymerase complexes can comprise a nanopore to provide isolated nanopore sequencing complexes. The methods relate to the positive and negative isolation of the polymerase complexes and/or nanopore sequencing complexes. Also disclosed is a nucleic acid adaptor for isolating active polymerase complexes, polymerase complexes comprising the nucleic acid adaptor, and methods for isolating active polymerase complexes using the nucleic acid adaptor.

公开(公告)号：US10669580B2

公开(公告)日：2020-06-02

申请号：US15684726

申请日：2017-08-23

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Dmitriy Gremyachinskiy ,  Peter Crisalli ,  Andrew Trans ,  Ashwini Bhat

IPC: C12Q1/68 ,  C07H19/04 ,  C07H19/10 ,  C07H21/04 ,  C12Q1/6869 ,  C12Q1/6823 ,  C07H19/06 ,  C07K14/00

Abstract: The present disclosure relates to compounds comprising a negatively-charged polymer moiety which is capable of entering a nanopore and upon entering a nanopore in the presence of positive ions results in an increased flow of the positive ions through the nanopore. The present disclosure provides methods of preparing the compounds and for their use as nanopore-detectable tags, in particular, for nanopore-based nucleic acid detection and sequencing.

公开(公告)号：US20170342485A1

公开(公告)日：2017-11-30

申请号：US15604611

申请日：2017-05-24

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Dmitriy Gremyachinskiy ,  Meng Taing ,  Aruna Ayer ,  Peter Crisalli

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C07H19/10 ,  G01N27/44791 ,  G01N33/48721 ,  C12Q2525/313 ,  C12Q2535/122 ,  C12Q2563/116 ,  C12Q2565/631

Abstract: The present disclosure relates to tagged multi-nucleotide compounds, which comprise a single tag moiety covalently linked to a plurality of nucleoside-5′-oligophosphate moieties. As disclosed herein, these tagged multi-nucleotide compounds have improved characteristics as polymerase substrates and can be used in a range of nucleic acid detection and sequencing methods, including nanopore sequencing-by-synthesis.

公开(公告)号：US11440933B2

公开(公告)日：2022-09-13

申请号：US17416186

申请日：2019-12-16

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Frank Bergmann ,  Peter Crisalli ,  Dieter Hiendl ,  Omid Khakshoor ,  Meng Taing

IPC: C07H19/06 ,  C07H19/048

Abstract: The present disclosure provides 3′ protected nucleotides, including those 3′ protected nucleotides having a detectable tag. Systems and methods of sequencing nucleic acids using the 3′ protected nucleotides are also disclosed, such as the sequencing of a nucleic acid using a nanopore or the sequencing of a nucleic acid via sequencing-by-synthesis.

公开(公告)号：US11293060B2

公开(公告)日：2022-04-05

申请号：US16596539

申请日：2019-10-08

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Frank Bergmann ,  Ian Quang Chau ,  Peter Crisalli ,  Hannah Marie Kallewaard-Lum ,  Hannes Kuchelmeister ,  Sebastian Johannes Pomplun ,  Hasham Saqib ,  Christian Wellner

IPC: C12Q1/68 ,  C12Q1/6869 ,  C07D295/13 ,  C07D487/04 ,  C07H21/02 ,  C07H21/04 ,  A61K47/68

Abstract: The present invention relates to improving the processing rate of a sequencing reaction, for example in a nanopore sequencing reaction, by means of using improved nucleoside-tags. The tags are linked to the nucleoside phosphate via a Pictet Spengler reaction. Exemplary sequencing reactions that are improved by the present methods include nanopore-based nucleic acid sequencing-by-synthesis reactions.

公开(公告)号：US20210317521A1

公开(公告)日：2021-10-14

申请号：US17229406

申请日：2021-04-13

Applicant: Roche Sequencing Solutions, Inc.

Inventor： Dmitriy Gremyachinskiy ,  Peter Crisalli ,  Andrew Trans ,  Ashwini Bhat

IPC: C12Q1/6869 ,  C12Q1/6823 ,  C07H21/04 ,  C07H19/10 ,  C07H19/06 ,  C07K14/00

Abstract: The present disclosure relates to compounds comprising a negatively-charged polymer moiety which is capable of entering a nanopore and upon entering a nanopore in the presence of positive ions results in an increased flow of the positive ions through the nanopore. The present disclosure provides methods of preparing the compounds and for their use as nanopore-detectable tags, in particular, for nanopore-based nucleic acid detection and sequencing.