公开(公告)号：US10577653B2

公开(公告)日：2020-03-03

申请号：US15630342

申请日：2017-06-22

申请人： Roche Sequencing Solutions, Inc.

发明人： Geoffrey Barrall ,  Jason Komadina ,  Marcin Rojek

IPC分类号： C12Q1/6874 ,  C12Q1/6869 ,  G01N27/447 ,  G01N33/487

摘要： A method of analyzing a molecule is disclosed. A lipid bilayer is formed such that it divides a first reservoir characterized by a first reservoir osmolarity from a second reservoir characterized by a second reservoir osmolarity. An electrolyte solution is flowed to the first reservoir that tends to make a first change to a ratio of the first reservoir osmolarity to the second reservoir osmolarity. A voltage is applied across the lipid bilayer, wherein the lipid bilayer is inserted with a nanopore, and wherein a net transfer of ions between the first reservoir and the second reservoir tends to make a second change to the ratio of the first reservoir osmolarity to the second reservoir osmolarity, and wherein the first change to the ratio and the second change to the ratio tends to counter-balance each other.

公开(公告)号：US10947590B2

公开(公告)日：2021-03-16

申请号：US16776320

申请日：2020-01-29

申请人： Roche Sequencing Solutions, Inc.

发明人： Geoffrey Barrall ,  Jason Komadina ,  Marcin Rojek

IPC分类号： C12Q1/6874 ,  C12Q1/6869 ,  G01N27/447 ,  G01N33/487

摘要： A method of analyzing a molecule is disclosed. A lipid bilayer is formed such that it divides a first reservoir characterized by a first reservoir osmolarity from a second reservoir characterized by a second reservoir osmolarity. An electrolyte solution is flowed to the first reservoir that tends to make a first change to a ratio of the first reservoir osmolarity to the second reservoir osmolarity. A voltage is applied across the lipid bilayer, wherein the lipid bilayer is inserted with a nanopore, and wherein a net transfer of ions between the first reservoir and the second reservoir tends to make a second change to the ratio of the first reservoir osmolarity to the second reservoir osmolarity, and wherein the first change to the ratio and the second change to the ratio tends to counter-balance each other.

公开(公告)号：US11739380B2

公开(公告)日：2023-08-29

申请号：US17200634

申请日：2021-03-12

申请人： Roche Sequencing Solutions, Inc.

发明人： Geoffrey Barrall ,  Jason Komadina ,  Marcin Rojek

IPC分类号： C12Q1/6874 ,  C12Q1/6869 ,  G01N27/447 ,  G01N33/487

CPC分类号： C12Q1/6874 ,  C12Q1/6869 ,  G01N27/44782 ,  G01N27/44791 ,  G01N33/48721 ,  C12Q1/6869 ,  C12Q2523/303 ,  C12Q2527/15 ,  C12Q2565/631

摘要： A method of analyzing a molecule is disclosed. A lipid bilayer is formed such that it divides a first reservoir characterized by a first reservoir osmolarity from a second reservoir characterized by a second reservoir osmolarity. An electrolyte solution is flowed to the first reservoir that tends to make a first change to a ratio of the first reservoir osmolarity to the second reservoir osmolarity. A voltage is applied across the lipid bilayer, wherein the lipid bilayer is inserted with a nanopore, and wherein a net transfer of ions between the first reservoir and the second reservoir tends to make a second change to the ratio of the first reservoir osmolarity to the second reservoir osmolarity, and wherein the first change to the ratio and the second change to the ratio tends to counter-balance each other.