公开(公告)号：US20240149536A1

公开(公告)日：2024-05-09

申请号：US18545879

申请日：2023-12-19

申请人： Roche Sequencing Solutions, Inc.

发明人： Roger J.A. Chen ,  Randy Davis

IPC分类号： B29C67/20 ,  B82Y5/00 ,  C12Q1/6869 ,  G01N33/487

CPC分类号： B29C67/20 ,  B82Y5/00 ,  C12Q1/6869 ,  G01N33/48721 ,  B82Y10/00

摘要： Techniques for forming a nanopore in a lipid bilayer are described herein. In one example, an agitation stimulus level such as an electrical agitation stimulus is applied to a lipid bilayer wherein the agitation stimulus level tends to facilitate the formation of nanopores in the lipid bilayer. In some embodiments, a change in an electrical property of the lipid bilayer resulting from the formation of the nanopore in the lipid bilayer is detected, and a nanopore has formed in the lipid bilayer is determined based on the detected change in the lipid bilayer electrical property.

公开(公告)号：US10926486B2

公开(公告)日：2021-02-23

申请号：US16179791

申请日：2018-11-02

申请人： Roche Sequencing Solutions, Inc.

发明人： Roger J. A. Chen ,  Randy Davis

IPC分类号： G01N27/327 ,  B29C67/20 ,  B82Y5/00 ,  C12Q1/6869 ,  G01N33/487 ,  B82Y10/00 ,  B82Y40/00

摘要： Techniques for forming a nanopore in a lipid bilayer are described herein. In one example, an agitation stimulus level such as an electrical agitation stimulus is applied to a lipid bilayer wherein the agitation stimulus level tends to facilitate the formation of nanopores in the lipid bilayer. In some embodiments, a change in an electrical property of the lipid bilayer resulting from the formation of the nanopore in the lipid bilayer is detected, and a nanopore has formed in the lipid bilayer is determined based on the detected change in the lipid bilayer electrical property.

公开(公告)号：US11884024B2

公开(公告)日：2024-01-30

申请号：US17303024

申请日：2021-05-18

申请人： Roche Sequencing Solutions, Inc.

发明人： Roger J. A. Chen ,  Randy Davis

IPC分类号： B29C67/20 ,  B82Y5/00 ,  C12Q1/6869 ,  G01N33/487 ,  B82Y10/00 ,  B82Y40/00

CPC分类号： B29C67/20 ,  B82Y5/00 ,  C12Q1/6869 ,  G01N33/48721 ,  B82Y10/00 ,  B82Y40/00 ,  Y10S977/797 ,  Y10S977/90 ,  Y10S977/942 ,  C12Q1/6869 ,  C12Q2565/631

摘要： Techniques for forming a nanopore in a lipid bilayer are described herein. In one example, an agitation stimulus level such as an electrical agitation stimulus is applied to a lipid bilayer wherein the agitation stimulus level tends to facilitate the formation of nanopores in the lipid bilayer. In some embodiments, a change in an electrical property of the lipid bilayer resulting from the formation of the nanopore in the lipid bilayer is detected, and a nanopore has formed in the lipid bilayer is determined based on the detected change in the lipid bilayer electrical property.

公开(公告)号：US20210268749A1

公开(公告)日：2021-09-02

申请号：US17303024

申请日：2021-05-18

申请人： Roche Sequencing Solutions, Inc.

发明人： Roger J.A. Chen ,  Randy Davis

IPC分类号： B29C67/20 ,  B82Y5/00 ,  C12Q1/6869 ,  G01N33/487

摘要： Techniques for forming a nanopore in a lipid bilayer are described herein. In one example, an agitation stimulus level such as an electrical agitation stimulus is applied to a lipid bilayer wherein the agitation stimulus level tends to facilitate the formation of nanopores in the lipid bilayer. In some embodiments, a change in an electrical property of the lipid bilayer resulting from the formation of the nanopore in the lipid bilayer is detected, and a nanopore has formed in the lipid bilayer is determined based on the detected change in the lipid bilayer electrical property.

公开(公告)号：US11027502B2

公开(公告)日：2021-06-08

申请号：US16179800

申请日：2018-11-02

申请人： Roche Sequencing Solutions, Inc.

发明人： Roger J. A. Chen ,  Randy Davis

IPC分类号： B29C67/20 ,  B82Y5/00 ,  C12Q1/6869 ,  G01N33/487 ,  B82Y10/00 ,  B82Y40/00

摘要： Techniques for forming a nanopore in a lipid bilayer are described herein. In one example, an agitation stimulus level such as an electrical agitation stimulus is applied to a lipid bilayer wherein the agitation stimulus level tends to facilitate the formation of nanopores in the lipid bilayer. In some embodiments, a change in an electrical property of the lipid bilayer resulting from the formation of the nanopore in the lipid bilayer is detected, and a nanopore has formed in the lipid bilayer is determined based on the detected change in the lipid bilayer electrical property.