公开(公告)号：US06281017B1

公开(公告)日：2001-08-28

申请号：US09491362

申请日：2000-01-26

申请人： Rodney B. Croteau ,  Bernd M. Lange

发明人： Rodney B. Croteau ,  Bernd M. Lange

IPC分类号： C12N1553

CPC分类号： C12N9/0006

摘要： The present invention relates to isolated DNA sequences which code for the expression of plant 1-deoxy-D-xylulose-5-phosphate reductoisomerase protein, such as the sequence presented in SEQ ID NO:1 which encodes a 1-deoxy-D-xylulose-5-phosphate reductoisomerase protein from peppermint (Mentha x piperita). Additionally, the present invention relates to isolated plant 1-deoxy-D-xylulose-5-phosphate reductoisomerase protein. In other aspects, the present invention is directed to replicable recombinant cloning vehicles comprising a nucleic acid sequence which codes for a plant 1-deoxy-D-xylulose-5-phosphate reductoisomerase, to modified host cells transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence of the invention.

摘要翻译： 本发明涉及编码植物1-脱氧-D-木酮糖-5-磷酸还原异构酶蛋白质的表达的分离的DNA序列，例如编码1-脱氧-D-木酮糖的SEQ ID NO：1所示的序列 -5-薄荷薄荷（Mentha x piperita）的5-磷酸还原异构酶蛋白。 另外，本发明涉及分离的植物1-脱氧-D-木酮糖-5-磷酸还原异构酶蛋白。 在其它方面，本发明涉及可复制的重组克隆载体，其包含编码植物1-脱氧-D-木酮糖-5-磷酸还原异构酶的核酸序列转化，转染，感染和/或注射的修饰的宿主细胞 与本发明的重组克隆载体和/或DNA序列。

公开(公告)号：US06420159B2

公开(公告)日：2002-07-16

申请号：US09874562

申请日：2001-06-04

申请人： Rodney B. Croteau ,  Bernd M. Lange

发明人： Rodney B. Croteau ,  Bernd M. Lange

IPC分类号： C12N990

CPC分类号： C12N9/0006

摘要： The present invention relates to isolated DNA sequences which code for the expression of plant 1-deoxy-D-xylulose-5-phosphate reductoisomerase protein, such as the sequence presented in SEQ ID NO:1 which encodes a 1-deoxy-D-xylulose-5-phosphate reductoisomerase protein from peppermint (Mentha x piperita). Additionally, the present invention relates to isolated plant 1-deoxy-D-xylulose-5-phosphate reductoisomerase protein. In other aspects, the present invention is directed to replicable recombinant cloning vehicles comprising a nucleic acid sequence which codes for a plant 1-deoxy-D-xylulose-5-phosphate reductoisomerase, to modified host cells transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence of the invention.

摘要翻译： 本发明涉及编码植物1-脱氧-SMALLCAPS> D D - 5-羟基-5-磷酸还原异构酶蛋白。 此外，本发明涉及分离的植物1-脱氧-β-氧化物-5-磷酸还原异构酶蛋白。 在其它方面，本发明涉及可复制的重组克隆载体，其包含编码植物1-脱氧-SMALLCAPS> D / /SMALLCAPSα-羟基-5-磷酸还原异构酶的核酸序列，转化的修饰的宿主细胞， 转染，感染和/或注射本发明的重组克隆载体和/或DNA序列。

公开(公告)号：US06235514B1

公开(公告)日：2001-05-22

申请号：US09434774

申请日：1999-11-04

申请人： Rodney B. Croteau ,  Bernd M. Lange

发明人： Rodney B. Croteau ,  Bernd M. Lange

IPC分类号： C12N900

CPC分类号： C12N9/12

摘要： A cDNA encoding isopentenyl monophosphate kinase (IPK) from peppermint (Mentha x piperita) has been isolated and sequenced, and the corresponding amino acid sequence has been determined. Accordingly, an isolated DNA sequence (SEQ ID NO:1) is provided which codes for the expression of isopentenyl monophosphate kinase (SEQ ID NO:2), from peppermint (Mentha x piperita). In other aspects, replicable recombinant cloning vehicles are provided which code for isopentenyl monophosphate kinase, or for a base sequence sufficiently complementary to at least a portion of isopentenyl monophosphate kinase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding isopentenyl monophosphate kinase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant isopentenyl monophosphate kinase that may be used to facilitate its production, isolation and purification in significant amounts. Recombinant isopentenyl monophosphate kinase may be used to obtain expression or enhanced expression of isopentenyl monophosphate kinase in plants in order to enhance the production of isopentenyl monophosphate kinase, or isoprenoids derived therefrom, or may be otherwise employed for the regulation or expression of isopentenyl monophosphate kinase, or the production of its products.

摘要翻译： 已经分离出来自薄荷（Mentha x piperita）的编码异戊烯基单磷酸激酶（IPK）的cDNA并进行测序，并确定了相应的氨基酸序列。 因此，提供了从薄荷（Mentha x piperita）编码异戊烯基单磷酸激酶（SEQ ID NO：2）的表达的分离的DNA序列（SEQ ID NO：1）。 在其它方面，提供了编码异戊烯基单磷酸激酶或与至少一部分异戊烯基单磷酸激酶DNA或RNA充分互补的碱基序列的可重复的重组克隆载体，以使其能够进行杂交。 在其它方面，提供已经用编码异戊烯基单磷酸激酶的重组克隆载体和/或DNA序列转化，转染，感染和/或注射的修饰的宿主细胞。 因此，提供了用于重组表达上述重组异戊烯基单磷酸激酶的系统和方法，其可用于促进其显着量的产生，分离和纯化。 可以使用重组异戊烯基单磷酸激酶来获得植物中异戊烯基单磷酸激酶的表达或增强的表达，以增强异戊烯基单磷酸激酶或衍生自其的类异戊二烯激酶的产生，或者可以用于调节或表达异戊烯基单磷酸激酶， 或其产品的生产。