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    1-deoxy-D-xylulose-5-phosphate reductoisomerases, and methods of use
    1.
    发明授权
    1-deoxy-D-xylulose-5-phosphate reductoisomerases, and methods of use 有权
    1-脱氧-D-木酮糖-5-磷酸还原异构酶及其使用方法

    公开(公告)号：US06420159B2

    公开(公告)日：2002-07-16

    申请号：US09874562

    申请日：2001-06-04

    申请人： Rodney B. Croteau ,  Bernd M. Lange

    发明人： Rodney B. Croteau ,  Bernd M. Lange

    IPC分类号： C12N990

    CPC分类号： C12N9/0006

    摘要： The present invention relates to isolated DNA sequences which code for the expression of plant 1-deoxy-D-xylulose-5-phosphate reductoisomerase protein, such as the sequence presented in SEQ ID NO:1 which encodes a 1-deoxy-D-xylulose-5-phosphate reductoisomerase protein from peppermint (Mentha x piperita). Additionally, the present invention relates to isolated plant 1-deoxy-D-xylulose-5-phosphate reductoisomerase protein. In other aspects, the present invention is directed to replicable recombinant cloning vehicles comprising a nucleic acid sequence which codes for a plant 1-deoxy-D-xylulose-5-phosphate reductoisomerase, to modified host cells transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence of the invention.

    摘要翻译： 本发明涉及编码植物1-脱氧-SMALLCAPS> D D - 5-羟基-5-磷酸还原异构酶蛋白。 此外，本发明涉及分离的植物1-脱氧-β-氧化物-5-磷酸还原异构酶蛋白。 在其它方面，本发明涉及可复制的重组克隆载体，其包含编码植物1-脱氧-SMALLCAPS> D / /SMALLCAPSα-羟基-5-磷酸还原异构酶的核酸序列，转化的修饰的宿主细胞， 转染，感染和/或注射本发明的重组克隆载体和/或DNA序列。

    Nucleic acid molecules encoding isopentenyl monophosphate kinase, and methods of use
    2.
    发明授权
    Nucleic acid molecules encoding isopentenyl monophosphate kinase, and methods of use 有权
    编码异戊烯基单磷酸激酶的核酸分子及其使用方法

    公开(公告)号：US06235514B1

    公开(公告)日：2001-05-22

    申请号：US09434774

    申请日：1999-11-04

    申请人： Rodney B. Croteau ,  Bernd M. Lange

    发明人： Rodney B. Croteau ,  Bernd M. Lange

    IPC分类号： C12N900

    CPC分类号： C12N9/12

    摘要： A cDNA encoding isopentenyl monophosphate kinase (IPK) from peppermint (Mentha x piperita) has been isolated and sequenced, and the corresponding amino acid sequence has been determined. Accordingly, an isolated DNA sequence (SEQ ID NO:1) is provided which codes for the expression of isopentenyl monophosphate kinase (SEQ ID NO:2), from peppermint (Mentha x piperita). In other aspects, replicable recombinant cloning vehicles are provided which code for isopentenyl monophosphate kinase, or for a base sequence sufficiently complementary to at least a portion of isopentenyl monophosphate kinase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding isopentenyl monophosphate kinase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant isopentenyl monophosphate kinase that may be used to facilitate its production, isolation and purification in significant amounts. Recombinant isopentenyl monophosphate kinase may be used to obtain expression or enhanced expression of isopentenyl monophosphate kinase in plants in order to enhance the production of isopentenyl monophosphate kinase, or isoprenoids derived therefrom, or may be otherwise employed for the regulation or expression of isopentenyl monophosphate kinase, or the production of its products.

    摘要翻译： 已经分离出来自薄荷（Mentha x piperita）的编码异戊烯基单磷酸激酶（IPK）的cDNA并进行测序，并确定了相应的氨基酸序列。 因此，提供了从薄荷（Mentha x piperita）编码异戊烯基单磷酸激酶（SEQ ID NO：2）的表达的分离的DNA序列（SEQ ID NO：1）。 在其它方面，提供了编码异戊烯基单磷酸激酶或与至少一部分异戊烯基单磷酸激酶DNA或RNA充分互补的碱基序列的可重复的重组克隆载体，以使其能够进行杂交。 在其它方面，提供已经用编码异戊烯基单磷酸激酶的重组克隆载体和/或DNA序列转化，转染，感染和/或注射的修饰的宿主细胞。 因此，提供了用于重组表达上述重组异戊烯基单磷酸激酶的系统和方法，其可用于促进其显着量的产生，分离和纯化。 可以使用重组异戊烯基单磷酸激酶来获得植物中异戊烯基单磷酸激酶的表达或增强的表达，以增强异戊烯基单磷酸激酶或衍生自其的类异戊二烯激酶的产生，或者可以用于调节或表达异戊烯基单磷酸激酶， 或其产品的生产。

    1-deoxy-d-xylulose-5-phosphate reductoisomerases and method of use
    3.
    发明授权
    1-deoxy-d-xylulose-5-phosphate reductoisomerases and method of use 有权
    1-脱氧-D-木酮糖-5-磷酸还原异构酶及其使用方法

    公开(公告)号：US06281017B1

    公开(公告)日：2001-08-28

    申请号：US09491362

    申请日：2000-01-26

    申请人： Rodney B. Croteau ,  Bernd M. Lange

    发明人： Rodney B. Croteau ,  Bernd M. Lange

    IPC分类号： C12N1553

    CPC分类号： C12N9/0006

    摘要： The present invention relates to isolated DNA sequences which code for the expression of plant 1-deoxy-D-xylulose-5-phosphate reductoisomerase protein, such as the sequence presented in SEQ ID NO:1 which encodes a 1-deoxy-D-xylulose-5-phosphate reductoisomerase protein from peppermint (Mentha x piperita). Additionally, the present invention relates to isolated plant 1-deoxy-D-xylulose-5-phosphate reductoisomerase protein. In other aspects, the present invention is directed to replicable recombinant cloning vehicles comprising a nucleic acid sequence which codes for a plant 1-deoxy-D-xylulose-5-phosphate reductoisomerase, to modified host cells transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence of the invention.

    摘要翻译： 本发明涉及编码植物1-脱氧-D-木酮糖-5-磷酸还原异构酶蛋白质的表达的分离的DNA序列，例如编码1-脱氧-D-木酮糖的SEQ ID NO：1所示的序列 -5-薄荷薄荷（Mentha x piperita）的5-磷酸还原异构酶蛋白。 另外，本发明涉及分离的植物1-脱氧-D-木酮糖-5-磷酸还原异构酶蛋白。 在其它方面，本发明涉及可复制的重组克隆载体，其包含编码植物1-脱氧-D-木酮糖-5-磷酸还原异构酶的核酸序列转化，转染，感染和/或注射的修饰的宿主细胞 与本发明的重组克隆载体和/或DNA序列。

    Nucleic acids encoding Taxus geranylgeranyl diphosphate synthase, and methods of use
    4.
    发明授权
    Nucleic acids encoding Taxus geranylgeranyl diphosphate synthase, and methods of use 有权
    编码红豆杉香叶基二磷酸合成酶的核酸及其使用方法

    公开(公告)号：US6043072A

    公开(公告)日：2000-03-28

    申请号：US187050

    申请日：1998-11-05

    申请人： Rodney B. Croteau ,  Jerry L. Hefner

    发明人： Rodney B. Croteau ,  Jerry L. Hefner

    IPC分类号： C12N15/09 ,  C12N5/10 ,  C12N9/10 ,  C12N15/82 ,  C12R1/91 ,  C07H21/04 ,  C07K1/00 ,  C12N1/20 ,  C12N15/00

    CPC分类号： C12N9/1085 ,  C12N15/8243 ,  C12Y205/01029

    摘要： A cDNA encoding geranylgeranyl diphosphate synthase from Canadian Yew (Taxus canadensis) has been isolated and sequenced, and the corresponding amino acid sequence has been determined. Accordingly, an isolated DNA sequence (SEQ ID NO:1) is provided which codes for the expression of geranylgeranyl diphosphate synthase (SEQ ID NO:2), from Canadian Yew (Taxus canadensis). In other aspects, replicable recombinant cloning vehicles are provided which code for geranylgeranyl diphosphate synthase, or for a base sequence sufficiently complementary to at least a portion of geranylgeranyl diphosphate synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding geranylgeranyl diphosphate synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant geranylgeranyl diphosphate synthase that may be used to facilitate its production, isolation and purification in significant amounts. Recombinant geranylgeranyl diphosphate synthase may be used to obtain expression or enhanced expression of geranylgeranyl diphosphate synthase in plants in order to enhance the production of geranylgeranyl diphosphate, or diterpenes derived therefrom, or may be otherwise employed for the regulation or expression of geranylgeranyl diphosphate synthase, or the production of its products.

    摘要翻译： 已经分离并测序了来自加拿大红豆杉（Taxus canadensis）的编码香叶基香叶基二磷酸合成酶的cDNA，并测定了相应的氨基酸序列。 因此，提供编码来自加拿大红豆杉（Taxus canadensis）的香叶基香叶基二磷酸合成酶（SEQ ID NO：2）的表达的分离的DNA序列（SEQ ID NO：1）。 在其它方面，提供编码香叶基香叶基二磷酸合酶的可重复克隆载体，或与至少一部分香叶基香叶基二磷酸合成酶DNA或RNA足够互补的碱基序列，以使其能够进行杂交。 在另一方面，提供已经用编码香叶基香叶基二磷酸合成酶的重组克隆载体和/或DNA序列转化，转染，感染和/或注射的修饰的宿主细胞。 因此，提供了用于重组表达上述重组er牛儿基ger牛儿基二磷酸合成酶的系统和方法，其可用于促进其显着量的产生，分离和纯化。 可以使用重组er牛儿基ger牛儿基二磷酸合成酶在植物中获得表达或增强er牛儿基ger牛儿基二磷酸合成酶的表达，以增强er牛儿基ger牛儿基二磷酸酯的产生，或由此衍生的二萜烯的生产，或可用于调节或表达香叶基香叶基二磷酸合成酶，或 其产品的生产。

    P450 oxygenases and methods of use
    9.
    发明授权
    P450 oxygenases and methods of use 有权
    P450加氧酶及使用方法

    公开(公告)号：US07402417B2

    公开(公告)日：2008-07-22

    申请号：US10565233

    申请日：2004-07-21

    申请人： Rodney B. Croteau ,  Robert Long ,  Stefan Jennewein

    发明人： Rodney B. Croteau ,  Robert Long ,  Stefan Jennewein

    IPC分类号： C12N9/02 ,  C12N1/20 ,  C12N15/00 ,  C12P7/66 ,  C12P21/04 ,  C12Q1/26 ,  C07H21/04 ,  C12Q1/68 ,  C12Q1/00

    CPC分类号： C12N9/0069

    摘要： This disclosure provides the nucleic acid and protein sequences of novel P450 oxygenases that utilize, at least, taxoid substrates, including taxadiene isomers. The disclosed oxygenases, such as a taxoid 5α-hydroxylase, hydroxylate, at least, the C5 position of a taxoid. Also provided are methods of introducing oxygen at the C5 position of a taxoid and methods of producing the anti-cancer drug, paclitaxel (also known as Taxol™), and other taxoids, such as paclitaxel intermediates.

    摘要翻译： 本公开提供使用至少紫杉烷底物（包括紫杉二烯异构体）的新型P450加氧酶的核酸和蛋白质序列。 公开的加氧酶，例​​如紫杉烷类5α-羟化酶，羟基化物，至少是紫杉烷的C5位置。 还提供了在紫杉烷的C5位置引入氧的方法和制备抗癌药物紫杉醇（也称为Taxol TM）以及其他类紫杉醇如紫杉醇中间体的方法。

    Compositions and methods for Taxol biosynthesis
    10.
    发明授权
    Compositions and methods for Taxol biosynthesis 失效
    紫杉醇生物合成的组成和方法

    公开(公告)号：US06610527B1

    公开(公告)日：2003-08-26

    申请号：US09593253

    申请日：2000-06-13

    申请人： Rodney B. Croteau ,  Mark R. Wildung

    发明人： Rodney B. Croteau ,  Mark R. Wildung

    IPC分类号： C12N988

    CPC分类号： C12N9/88 ,  C07K2319/00 ,  C12N9/90 ,  C12N15/8243

    摘要： The taxadiene synthase gene of Pacific yew has been cloned and its nucleic acid and polypeptide sequence is presented. Truncation or removal of the transit peptide increases expression of the cloned taxadiene synthase gene expression in E. coli cells.

    摘要翻译： 已经克隆了太平洋红豆杉的紫杉二烯合酶基因，并提出了其核酸和多肽序列。 转运肽的截断或去除增加了在大肠杆菌细胞中克隆的二聚体合成酶基因表达的表达。