公开(公告)号：US06936215B1

公开(公告)日：2005-08-30

申请号：US08703608

申请日：1996-08-27

申请人： Ronald R. Price ,  Joel M. Schnur ,  Banahalli R. Ratna ,  Mark S. Spector

发明人： Ronald R. Price ,  Joel M. Schnur ,  Banahalli R. Ratna ,  Mark S. Spector

IPC分类号： B01J13/00

CPC分类号： C11B3/00

摘要： The wall thickness of lipid microtubules are controlled by selecting a methanol/water system and determining the required amount of a lipid to form the desired wall thickness. The lipid is dissolved in a small portion of the heated methanol and that clear solution is added to the remaining amount of the heated methanol/water system. By slowly cooling the solution, microtubules are formed which have the desired wall thickness. Preferred microtubules have a wall thickness of just 2 bilayers and they are robust so they can be further coated. They can be made with a large aspect ratio and with lengths of greater than 250 microns. The process permits production of microtubules in very high yields.

摘要翻译： 通过选择甲醇/水系统并确定所需量的脂质以形成所需的壁厚来控制脂质微管的壁厚。 将脂质溶解在一小部分加热的甲醇中，并将澄清溶液加入剩余量的加热的甲醇/水体系中。 通过缓慢冷却溶液，形成具有所需壁厚的微管。 优选的微管的壁厚仅为2双层，并且它们是坚固的，因此它们可以被进一步涂覆。 它们可以制成具有大的纵横比并且长度大于250微米。 该方法允许以非常高的产率生产微管。

公开(公告)号：US07785770B2

公开(公告)日：2010-08-31

申请号：US11444819

申请日：2006-05-19

申请人： Mark S. Spector ,  David A. Stenger ,  Charles H. Patterson, Jr. ,  Brett D. Martin ,  Paul T. Charles

发明人： Mark S. Spector ,  David A. Stenger ,  Charles H. Patterson, Jr. ,  Brett D. Martin ,  Paul T. Charles

IPC分类号： C12Q1/00

CPC分类号： G01N33/548 ,  G01N33/54353

摘要： The use of sugar-containing hydrogels as very highly porous, aqueous support material for the immobilization of oligonucleotides, peptides, proteins, antigens, antibodies, polysaccharides, and other biomolecules for sensor applications. Unusually large sizes of interconnected pores allow large target molecules to pass rapidly into and through the gel and bind to immobilized biomolecules. Sugar-containing hydrogels have extremely low non-specific absorption of labeled target molecules, providing low background levels. Some hydrogel materials do not have this type of homogeneous interconnected macroporosity, thus large target molecules cannot readily diffuse through them. Additionally, they nearly always experience non-specific absorption of labeled target molecules, limiting their usefulness in sensor applications. A method is provided for preparing sugar polyacrylate hydrogels with functional chemical groups which covalently bond oligonucleotides and peptides. A method for copolymerizing acrylate-terminated oligonucleotides with sugar acrylate monomers and diacrylate cross-linking agents is also provided.

摘要翻译： 使用含糖水凝胶作为用于固定寡核苷酸，肽，蛋白质，抗原，抗体，多糖和其他用于传感器应用的生物分子的非常高度多孔的水性支持材料。 异常大尺寸的相互连通的孔允许大的目标分子快速通过并通过凝胶并结合固定化的生物分子。 含糖水凝胶对标记的靶分子具有极低的非特异性吸收，提供低的背景水平。 一些水凝胶材料不具有这种类型的均匀相互连接的大孔隙度，因此大的靶分子不能容易地通过它们扩散。 此外，它们几乎总是经历对标记的靶分子的非特异性吸收，限制了它们在传感器应用中的用途。 提供了一种制备具有共价键合寡核苷酸和肽的功能化学基团的糖聚丙烯酸酯水凝胶的方法。 还提供了将丙烯酸酯封端的寡核苷酸与糖丙酯单体和二丙烯酸酯交联剂共聚的方法。

公开(公告)号：US07785769B2

公开(公告)日：2010-08-31

申请号：US10627143

申请日：2003-07-25

申请人： Mark S. Spector ,  David A. Stenger ,  Charles H. Patterson, Jr. ,  Brett D. Martin ,  Paul T. Charles

发明人： Mark S. Spector ,  David A. Stenger ,  Charles H. Patterson, Jr. ,  Brett D. Martin ,  Paul T. Charles

IPC分类号： C12Q1/00

CPC分类号： G01N33/548 ,  G01N33/54353

摘要： The use of sugar-containing hydrogels as very highly porous, aqueous support material for the immobilization of oligonucleotides, peptides, proteins, antigens, antibodies, polysaccharides, and other biomolecules for sensor applications. Unusually large sizes of interconnected pores allow large target molecules to pass rapidly into and through the gel and bind to immobilized biomolecules. Sugar-containing hydrogels have extremely low non-specific absorption of labeled target molecules, providing low background levels. Some hydrogel materials do not have this type of homogeneous interconnected macroporosity, thus large target molecules cannot readily diffuse through them. Additionally, they nearly always experience non-specific absorption of labeled target molecules, limiting their usefulness in sensor applications. A method is provided for preparing sugar polyacrylate hydrogels with functional chemical groups which covalently bond oligonucleotides and peptides. A method for copolymerizing acrylate-terminated oligonucleotides with sugar acrylate monomers and diacrylate cross-linking agents is also provided.

摘要翻译： 使用含糖水凝胶作为用于固定寡核苷酸，肽，蛋白质，抗原，抗体，多糖和其他用于传感器应用的生物分子的非常高度多孔的水性载体材料。 异常大尺寸的相互连通的孔允许大的目标分子快速通过并通过凝胶并结合固定化的生物分子。 含糖水凝胶对标记的靶分子具有极低的非特异性吸收，提供低的背景水平。 一些水凝胶材料不具有这种类型的均匀相互连接的大孔隙度，因此大的靶分子不能容易地通过它们扩散。 此外，它们几乎总是经历对标记的靶分子的非特异性吸收，限制了它们在传感器应用中的用途。 提供了一种制备具有共价键合寡核苷酸和肽的功能化学基团的糖聚丙烯酸酯水凝胶的方法。 还提供了将丙烯酸酯封端的寡核苷酸与糖丙酯单体和二丙烯酸酯交联剂共聚的方法。