公开(公告)号：US07199224B2

公开(公告)日：2007-04-03

申请号：US10423729

申请日：2003-04-25

申请人： Roy A Black ,  Charles Rauch ,  Carl J March ,  Douglas P Cerretti

发明人： Roy A Black ,  Charles Rauch ,  Carl J March ,  Douglas P Cerretti

IPC分类号： C07K16/00

CPC分类号： C12N9/6489 ,  C07K16/40

摘要： A metalloprotease that converts TNF-α from the 26 kD cell form to the 17 kD form has been isolated and purified and the cDNA sequence known. In particular, the protease has a molecular weight of approximately 80 kD. The isolated and purified protease is useful for designing an inhibitor thereof, and may find use as a therapeutic agent. Assays for detecting the protease-inhibiting activity of a molecule are also an aspect of the invention.

摘要翻译： 已经分离和纯化将来自26kD细胞形式的TNF-α转化为17kD形式的金属蛋白酶，并且已知cDNA序列。 特别地，蛋白酶的分子量约为80kD。 分离和纯化的蛋白酶可用于设计其抑制剂，可用作治疗剂。 用于检测分子的蛋白酶抑制活性的测定法也是本发明的一个方面。