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35 条记录 (耗时 0.04 秒)

    Antibodies that bind TNF-α converting enzyme
    1.
    发明授权
    Antibodies that bind TNF-α converting enzyme 失效
    结合TNF-α转化酶的抗体

    公开(公告)号：US07695948B2

    公开(公告)日：2010-04-13

    申请号：US11709436

    申请日：2007-02-21

    申请人： Roy A. Black ,  Charles Rauch ,  Carl J. March ,  Douglas P. Cerretti

    发明人： Roy A. Black ,  Charles Rauch ,  Carl J. March ,  Douglas P. Cerretti

    IPC分类号： C12N9/48

    CPC分类号： C12N9/6489 ,  C07K16/40

    摘要： A metalloprotease that converts TNF-α from the 26 kD cell form to the 17 kD form has been isolated and purified and the cDNA sequence known. In particular, the protease has a molecular weight of approximately 80 kD. The isolated and purified protease is useful for designing an inhibitor thereof, and may find use as a therapeutic agent. Assays for detecting the protease-inhibiting activity of a molecule are also an aspect of the invention.

    摘要翻译： 将TNF-α从26kD细胞形式转化为17kD形式的金属蛋白酶已被分离并纯化，cDNA序列已知。 特别地，蛋白酶的分子量约为80kD。 分离和纯化的蛋白酶可用于设计其抑制剂，可用作治疗剂。 用于检测分子的蛋白酶抑制活性的测定法也是本发明的一个方面。

    Antibodies that bind TNF-α converting enzyme
    2.
    发明授权
    Antibodies that bind TNF-α converting enzyme 失效
    结合TNF-α转换酶的抗体

    公开(公告)号：US07199224B2

    公开(公告)日：2007-04-03

    申请号：US10423729

    申请日：2003-04-25

    申请人： Roy A Black ,  Charles Rauch ,  Carl J March ,  Douglas P Cerretti

    发明人： Roy A Black ,  Charles Rauch ,  Carl J March ,  Douglas P Cerretti

    IPC分类号： C07K16/00

    CPC分类号： C12N9/6489 ,  C07K16/40

    摘要： A metalloprotease that converts TNF-α from the 26 kD cell form to the 17 kD form has been isolated and purified and the cDNA sequence known. In particular, the protease has a molecular weight of approximately 80 kD. The isolated and purified protease is useful for designing an inhibitor thereof, and may find use as a therapeutic agent. Assays for detecting the protease-inhibiting activity of a molecule are also an aspect of the invention.

    摘要翻译： 已经分离和纯化将来自26kD细胞形式的TNF-α转化为17kD形式的金属蛋白酶，并且已知cDNA序列。 特别地，蛋白酶的分子量约为80kD。 分离和纯化的蛋白酶可用于设计其抑制剂，可用作治疗剂。 用于检测分子的蛋白酶抑制活性的测定法也是本发明的一个方面。

    TNF-.alpha. converting enzyme
    5.
    发明授权
    TNF-.alpha. converting enzyme 失效
    TNF-α转化酶

    公开(公告)号：US6013466A

    公开(公告)日：2000-01-11

    申请号：US183275

    申请日：1998-10-30

    申请人： Roy A. Black ,  Charles Rauch ,  Carl J. March ,  Douglas P. Cerretti

    发明人： Roy A. Black ,  Charles Rauch ,  Carl J. March ,  Douglas P. Cerretti

    IPC分类号： C07K16/40 ,  C12N9/64 ,  C12Q1/37

    CPC分类号： C12N9/6489 ,  C07K16/40

    摘要： A metalloprotease that converts TNF-.alpha. from the 26 kD cell form to the 17 kD form has been isolated and purified and the cDNA sequence known. In particular, the protease has a molecular weight of approximately 80 kD. The isolated and purified protease is useful for designing an inhibitor thereof, and may find use as a therapeutic agent. Assays for detecting the protease-inhibiting activity of a molecule are also an aspect of the invention.

    摘要翻译： 已经分离和纯化将TNF-α从26kD细胞形式转化为17kD形式的金属蛋白酶，并且已知cDNA序列。 特别地，蛋白酶的分子量约为80kD。 分离和纯化的蛋白酶可用于设计其抑制剂，可用作治疗剂。 用于检测分子的蛋白酶抑制活性的测定法也是本发明的一个方面。

    Purification of the IL-2 receptor
    9.
    发明授权
    Purification of the IL-2 receptor 失效
    IL-2受体的纯化

    公开(公告)号：US5317087A

    公开(公告)日：1994-05-31

    申请号：US630331

    申请日：1990-10-22

    申请人： Douglas P. Cerretti ,  ; David J. Cosman ,  Steven K. Dower ,  Carl J. March ,  David L. Urdal ,  Alf D. Larsen

    发明人： Douglas P. Cerretti ,  ; David J. Cosman ,  Steven K. Dower ,  Carl J. March ,  David L. Urdal ,  Alf D. Larsen

    IPC分类号： C07K14/715 ,  C07K16/28 ,  C07K13/00 ,  C07K15/00

    CPC分类号： C07K14/7155 ,  C07K16/2866

    摘要： Interleukin-2 receptor derived from normal and malignant cells has been purified by use of various techniques including affinity chromatography in conjunction with a monoclonal antibody directed to the receptor. The purification process also includes reversed phased high performance liquid chromatography. By these techniques, interleukin-2 receptor has been purified to homogeneity. The high purification of the interleukin-2 receptor has made possible the sequencing of the amino acid residues at the N-terminal of this protein molecule. Double-stranded cDNA is prepared from polyadenylated RNA extracted from cell lines or other sources known to produce IL-2 receptor. The cDNA is inserted within a plasmid vector and then the recombinant plasmid employed to transform an appropriate host. Transformed hosts are identified and grouped into pools. Plasmid DNA prepared from these pools is hybridized with a labeled synthetic oligonucleotide probe corresponding to a portion of the amino acid sequence of the purified IL-2 receptor. The cDNA clone isolated with the probe is characterized by restriction enzyme mapping and sequenced by chain-termination method. The particular DNA clone that actually contains the gene coding for the functional IL-2 receptor is identified by expressing the clones in COS-7 monkey kidney cells and assaying for the expressed IL-2 receptor by its ability to bind IL-2 or a monoclonal antibody directed against the IL-2 receptor.

    摘要翻译： 来自正常和恶性细胞的白细胞介素-2受体已经通过使用各种技术进行纯化，包括亲和层析与针对受体的单克隆抗体。 纯化方法还包括反相高效液相色谱法。 通过这些技术，白细胞介素-2受体已经被纯化到均一性。 白细胞介素-2受体的高度纯化使得可以对该蛋白质分子的N-末端的氨基酸残基进行测序。 从从已知产生IL-2受体的细胞系或其他来源提取的聚腺苷酸化的RNA制备双链cDNA。 将cDNA插入质粒载体中，然后将重组质粒用于转化合适的宿主。 已识别转化的主机并将其分组到池中。 从这些库制备的质粒DNA与对应于纯化的IL-2受体的氨基酸序列的一部分的标记的合成寡核苷酸探针杂交。 用探针分离的cDNA克隆的特征在于限制酶作图并通过链终止法进行测序。 通过在COS-7猴肾细胞中表达克隆并通过其结合IL-2或单克隆抗体的能力测定表达的IL-2受体来鉴定实际含有编码功能性IL-2受体的基因的特定DNA克隆 针对IL-2受体的抗体。