公开(公告)号：US20190143002A1

公开(公告)日：2019-05-16

申请号：US16098994

申请日：2017-06-23

Applicant: SHISEIDO COMPANY, LTD.

Inventor： Mitsuhiro DENDA ,  Sumiko DENDA ,  Junichi KUMAMOTO ,  Yasuaki KOBAYASHI ,  Masaharu NAGAYAMA

IPC: A61L27/60 ,  C12N5/071 ,  B01L1/02 ,  C12N5/00

Abstract: A thick three-dimensional culture skin sheet is provided. The invention provides a three-dimensional culture skin sheet comprising at least epidermal cells and having concavoconvexities in at least one portion of the base section. The invention further provides a cell culturing vessel to be used for production of the three-dimensional culture skin sheet, having on at least one portion of the culture surface a porous film comprising convex sections for formation of concavoconvexities in the base section of the three-dimensional culture skin sheet. The invention still further provides a method for preparing a three-dimensional culture skin sheet using the cell culturing vessel.