公开(公告)号：US11123656B2

公开(公告)日：2021-09-21

申请号：US16609938

申请日：2018-06-21

Applicant: SHOWA DENKO K.K.

Inventor： Junji Sasuga ,  Hirobumi Aoki ,  Yuzuru Kokido

IPC: B01D15/32 ,  B01J20/285 ,  G01N30/34 ,  G01N30/50 ,  G01N30/72 ,  G01N30/74 ,  G01N30/88 ,  B01D15/16 ,  B01D15/42 ,  B01D15/20 ,  B01J20/32 ,  C07K1/20 ,  B01J20/28

Abstract: The present invention relates to a method of separating and analyzing a mixture of oligonucleotides, including performing liquid chromatography using a column packed with a packing material obtained by fixing a diol to a surface of each of porous particles formed of a crosslinked organic polymer. According to this method, the oligonucleotides can be separated and analyzed with higher sensitivity compared to cases where columns having silica gel as a base material are used. In addition, the column can be washed with an alkaline solution.