公开(公告)号：US20200283487A1

公开(公告)日：2020-09-10

申请号：US16063956

申请日：2016-12-21

Applicant: University of Southampton ,  Spiber Technologies AB

Inventor： Howard William Clark ,  Alastair Samuel Watson ,  Jens Madsen ,  Jan Johansson ,  Anna Rising ,  Nina Kronqvist ,  Kerstin Nordling

IPC: C07K14/435 ,  C07K14/705

Abstract: The present invention provides a fusion protein comprising: i) a solubility-enhancing moiety which is derived from the N-terminal (NT) fragment of a spider silk protein; and ii) a C-type lectin polypeptide. The present invention also provides a truncated SP-A polypeptide which lacks the N-terminal domain of full SP-A and is capable of trimerisation, and to the use of such a truncated SP-A polypeptide in treating or preventing a disease.

公开(公告)号：US11214601B2

公开(公告)日：2022-01-04

申请号：US16816486

申请日：2020-03-12

Applicant: SPIBER TECHNOLOGIES AB

Inventor： Jan Johansson ,  Anna Rising ,  Nina Kronqvist ,  Kerstin Nordling

IPC: C07K14/435 ,  C07K1/36

Abstract: A protein comprising a moiety of 100-160 amino acid residues having at least 70% identity with the N-terminal (NT) fragment of a spider silk protein, wherein the amino acid residue corresponding to position 40 in NT is selected from the group consisting of Lys, Arg and His; and wherein the amino acid residue corresponding to position 65 in NT is selected from the group consisting of Asp and Glu, is useful as a moiety in a fusion protein for enhancing the solubility of another moiety in the fusion protein, which is a desired protein or polypeptide.

公开(公告)号：US10604550B2

公开(公告)日：2020-03-31

申请号：US15490328

申请日：2017-04-18

Applicant: SPIBER TECHNOLOGIES AB

Inventor： Jan Johansson ,  Anna Rising ,  My Hedhammar ,  Kerstin Nordling

IPC: C07K14/435 ,  C12N15/62

Abstract: A method of producing a desired non-spidroin protein or polypeptide is comprising the steps of expressing in a suitable host a fusion protein, obtaining a mixture containing the fusion protein, and optionally isolating the fusion protein. The fusion protein is comprising at least one solubility-enhancing moiety which is derived from the N-terminal (NT) fragment of a spider silk protein. It is further comprising at least one moiety which is a desired non-spidroin protein or polypeptide. Each solubility-enhancing moiety is linked directly or indirectly to the desired protein or polypeptide moiety.