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    Engineered spider silk proteins and uses thereof
    1.
    发明授权
    Engineered spider silk proteins and uses thereof 有权

    公开(公告)号：US11524984B2

    公开(公告)日：2022-12-13

    申请号：US16314501

    申请日：2017-06-29

    Applicant: SPIBER TECHNOLOGIES AB

    Inventor： Anna Rising ,  Jan Johansson ,  Marlene Andersson

    IPC: C07K14/435 ,  B33Y70/00 ,  C12N5/00 ,  A61K38/00

    Abstract: A recombinant spider silk protein, consisting of no more than 800 amino acids, comprising a set of domains arranged according to the formula (NT)-REP-CT, wherein: the optional NT-domain, if present, comprises a sequence of 100 to 160 amino-acid residues derived from the N-terminal domain of a spider silk protein; the REP-domain comprises a sequence of 30 to 600 amino acid residues derived from the repetitive segment of a spider silk protein; and the CT-domain comprises a sequence of 70 to 120 amino acid residues derived from the C-terminal domain of a spider silk protein selected from: a sequence of 72 to 110 amino acid residues derived from the C-terminal domain of a spider silk protein, wherein the sequence comprises at least 7 residues independently selected from K, R, E and D; a sequence having at least 85% identity to SEQ ID NO: 15 or any one of SEQ ID NOs: 62-65 or 67-73; and a sequence having at least 70% identity to SEQ ID NOs: 64 or any one of SEQ ID NOs: 62-65 or 67-73, wherein the sequence comprises at least 7 residues independently selected from K, R, E and D.

    Charge-reversed N-terminal spider silk protein domain and uses thereof
    2.
    发明授权
    Charge-reversed N-terminal spider silk protein domain and uses thereof 有权

    公开(公告)号：US11214601B2

    公开(公告)日：2022-01-04

    申请号：US16816486

    申请日：2020-03-12

    Applicant: SPIBER TECHNOLOGIES AB

    Inventor： Jan Johansson ,  Anna Rising ,  Nina Kronqvist ,  Kerstin Nordling

    IPC: C07K14/435 ,  C07K1/36

    Abstract: A protein comprising a moiety of 100-160 amino acid residues having at least 70% identity with the N-terminal (NT) fragment of a spider silk protein, wherein the amino acid residue corresponding to position 40 in NT is selected from the group consisting of Lys, Arg and His; and wherein the amino acid residue corresponding to position 65 in NT is selected from the group consisting of Asp and Glu, is useful as a moiety in a fusion protein for enhancing the solubility of another moiety in the fusion protein, which is a desired protein or polypeptide.

    Production of proteins and polypeptides
    3.
    发明授权
    Production of proteins and polypeptides 有权

    公开(公告)号：US10604550B2

    公开(公告)日：2020-03-31

    申请号：US15490328

    申请日：2017-04-18

    Applicant: SPIBER TECHNOLOGIES AB

    Inventor： Jan Johansson ,  Anna Rising ,  My Hedhammar ,  Kerstin Nordling

    IPC: C07K14/435 ,  C12N15/62

    Abstract: A method of producing a desired non-spidroin protein or polypeptide is comprising the steps of expressing in a suitable host a fusion protein, obtaining a mixture containing the fusion protein, and optionally isolating the fusion protein. The fusion protein is comprising at least one solubility-enhancing moiety which is derived from the N-terminal (NT) fragment of a spider silk protein. It is further comprising at least one moiety which is a desired non-spidroin protein or polypeptide. Each solubility-enhancing moiety is linked directly or indirectly to the desired protein or polypeptide moiety.

    Spider silk proteins and methods for producing spider silk proteins
    5.
    发明授权
    Spider silk proteins and methods for producing spider silk proteins 有权
    蜘蛛丝蛋白和生产蜘蛛丝蛋白的方法

    公开(公告)号：US08729235B2

    公开(公告)日：2014-05-20

    申请号：US14086244

    申请日：2013-11-21

    Applicant: Spiber Technologies AB

    Inventor： Jan Johansson ,  Gäran Hjäm ,  Margareta Stark ,  Anna Rising ,  Stefan Grip ,  Wilhelm Engsträm ,  My Hedhammar

    IPC: C07K1/00 ,  C07K14/00 ,  C12N11/08 ,  C12M1/00 ,  C12P1/00 ,  A61F13/00 ,  A61K9/70 ,  A61L15/16

    CPC classification number: C07K14/43518 ,  A61K35/12 ,  A61K47/42 ,  D01F4/00

    Abstract: The invention provides an isolated major ampullate spidroin protein, which consists of from 150 to 420 amino acid residues and is defined by the formula REP-CT. REP is a repetitive, N-terminally derived protein fragment having from 80 to 300 amino acid residues. CT is a C-terminally derived protein fragment having from 70 to 120 amino acid residues. The invention further provides an isolated fusion protein consisting of a first protein fragment, which is a major ampullate spidroin protein, and a second protein fragment comprising a fusion partner and a cleavage agent recognition site. The first protein fragment is coupled via said cleavage agent recognition site to the fusion partner. The invention also provides a method of producing a major ampullate spidroin protein and polymers thereof.

    Abstract translation: 本发明提供了一种分离的主要的安瓿蛋白质宿主蛋白，其由150至420个氨基酸残基组成，并由式REP-CT定义。 REP是具有80至300个氨基酸残基的重复的N末端衍生的蛋白质片段。 CT是具有70至120个氨基酸残基的C末端衍生的蛋白质片段。 本发明进一步提供了由第一蛋白片段（其是主要的安非他索特灵素蛋白质）和包含融合配偶体和切割剂识别位点的第二蛋白质片段组成的分离的融合蛋白质。 第一个蛋白质片段通过所述切割剂识别位点连接到融合配偶体。 本发明还提供了一种生产主要的安非他命复合蛋白及其聚合物的方法。

    Engineered Spider Silk Proteins And Uses Thereof
    7.
    发明申请
    Engineered Spider Silk Proteins And Uses Thereof 审中-公开

    公开(公告)号：US20190248847A1

    公开(公告)日：2019-08-15

    申请号：US16314501

    申请日：2017-06-29

    Applicant: SPIBER TECHNOLOGIES AB

    Inventor： Anna Rising ,  Jan Johansson ,  Marlene Andersson

    IPC: C07K14/435 ,  C12N5/00 ,  B33Y70/00

    CPC classification number: C07K14/43518 ,  B33Y70/00 ,  C12N5/0018 ,  C12N5/0062 ,  C12N2513/00 ,  C12N2533/50

    Abstract: A recombinant spider silk protein, consisting of no more than 800 amino acids, comprising a set of domains arranged according to the formula (NT)-REP-CT, wherein: the optional NT-domain, if present, comprises a sequence of 100 to 160 amino-acid residues derived from the N-terminal domain of a spider silk protein; the REP-domain comprises a sequence of 30 to 600 amino acid residues derived from the repetitive segment of a spider silk protein; and the CT-domain comprises a sequence of 70 to 120 amino acid residues derived from the C-terminal domain of a spider silk protein selected from: a sequence of 72 to 110 amino acid residues derived from the C-terminal domain of a spider silk protein, wherein the sequence comprises at least 7 residues independently selected from K, R, E and D; a sequence having at least 85% identity to SEQ ID NO: 15 or any one of SEQ ID NO:s 62-65 or 67-73; and a sequence having at least 70% identity to SEQ. ID NO: 64 or any one of SEQ ID NO:s 62-65 or 67-73, wherein the sequence comprises at least 7 residues independently selected from K, R, E and D. Solutions, polymers, uses and methods of manufacture thereof.

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