公开(公告)号：US3857969A

公开(公告)日：1974-12-31

申请号：US43863074

申请日：1974-02-01

Applicant: STAMICARBON

Inventor： WIKEN T ,  BAKKER G

IPC: A23C19/032 ,  C12N9/58 ,  C12D13/10 ,  A23C19/00

CPC classification number: A23C19/0326 ,  C12N9/58 ,  Y10S435/931

Abstract: A process for producing an enzyme preparation capable of coagulating milk by cultivating a fungi of the group Mucor lamprosporus or a natural or synthetic variant or mutant thereof in a culture medium containing a sufficiency of available carbon, nitrogen and trace nutrients at a temperature of between 15* and 45*C, with the incubation under aerobic conditions for a period of between 2 and 12 days. The enzyme preparation is then recovered from the medium, for example, by extraction with water or in the dissolved state by filtration and dialysis. The enzyme preparations obtained have a high milk-coagulation activity with low proteolytic and lipolytic activity.

Abstract translation: 一种制备能够通过在含有足够的可用碳，氮和微量营养物的培养基中培养白色假丝酵母菌或其天然或合成的变体或突变体的霉菌来凝结牛奶的方法，该温度为15 DEG和45℃，在有氧条件下孵育2至12天。 然后从培养基中回收酶制剂，例如通过用水萃取或通过过滤和透析处于溶解状态。 所得酶制剂具有较高的乳凝固活性，蛋白水解和脂肪分解活性低。