REDUCED SODIUM CHEESES AND METHODS FOR MAKING SAME
    5.
    发明申请
    REDUCED SODIUM CHEESES AND METHODS FOR MAKING SAME 审中-公开
    减少的钠盐及其制备方法

    公开(公告)号:US20160227804A1

    公开(公告)日:2016-08-11

    申请号:US15023776

    申请日:2014-09-10

    申请人: NESTEC S.A.

    发明人: Praveen Upreti

    摘要: Reduced sodium cheeses and methods for making same are provided. The methods include adding rennet to inoculated milk without a delay from inoculation or with less of a delay from inoculation relative to known methods. The rennet is added to the milk within five minutes after addition of the bacteria, preferably within one minute or simultaneously. The methods use a higher pH at inoculation and/or at cutting and draining of curds to enable a reduction in the amount of sodium chloride added to the curds and thus accelerating the ripening of cheese. Delayed acidification achieved by extending a later stage of processing can obtain a similar final pH relative to known methods. The cheeses have reduced sodium without affecting the flavor profile of the cheese.

    摘要翻译: 提供了降低的钠奶酪及其制备方法。 所述方法包括向接种的牛奶中加入凝乳酶,而不是延迟接种或相对于已知方法接种的延迟较少。 在加入细菌后五分钟内将凝乳酶加入到牛奶中,优选在一分钟内或同时进行。 该方法在接种和/或切割和排出凝乳时使用较高的pH,以减少加入到凝乳中的氯化钠的量,从而加速干酪的成熟。 通过延长后期处理而实现的延迟酸化可以获得相对于已知方法的相似的最终pH。 奶酪减少了钠而不影响奶酪的风味。

    CHYMOSINE ENZYME VARIANTS
    7.
    发明申请

    公开(公告)号:US20150118356A1

    公开(公告)日:2015-04-30

    申请号:US14397866

    申请日:2013-05-03

    IPC分类号: A23C19/032 C12N9/64

    摘要: A variant polypeptide having chymosin activity, wherein the variant has an amino acid sequence which, when aligned with the chymosin comprising the sequence set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue corresponding to any of amino acids 2, 22, 40, 48, 50, 51, 53, 61, 62, 76, 88, 98, 99, 109, 112, 117, 125, 126, 135, 144, 160, 161, 163, 187, 189, 194, 200, 201, 202, 203, 221, 223, 240, 242, 244, 254, 267, 271, 273, 278, 280, 284, 289, 292, 294 or 295 said positions being defined with reference to SEQ ID NO: 2 and wherein the variant has one or more altered properties as compared with a reference polypeptide having chymosin activity. Such a variant polypeptide may be used in the preparation of a cheese.

    摘要翻译: 具有凝乳酶活性的变体多肽,其中所述变体具有当与包含SEQ ID NO:2所示的序列的凝乳酶比对时的氨基酸序列包含对应于任何氨基酸的氨基酸残基的至少一个取代 2,22,40,48,50,51,53,61,62,76,88,98,99,109,112,117,125,126,135,144,160,161,163,187, 194,2200,201,202,203,221,223,240,242,244,254,267,271,273,278,280,284,289,292,294或295表示所述位置是参照SEQ ID NO:2,其中与具有凝乳酶活性的参照多肽相比,变体具有一个或多个改变的特性。 这种变体多肽可用于制备奶酪。

    Microbially derived enzymes having enhanced milk clotting activity and
method of producing same
    8.
    发明授权
    Microbially derived enzymes having enhanced milk clotting activity and method of producing same 失效
    具有增强的乳凝活性的微生物来源的酶及其生产方法

    公开(公告)号:US06127142A

    公开(公告)日:2000-10-03

    申请号:US849922

    申请日:1997-08-14

    CPC分类号: C12N9/58 A23C19/0326

    摘要: A method of producing a milk clotting enzyme including the steps of (a) fermenting a strain of Rhizomucor miehei or Aspergillus oryzae to form a fermentation product having a glycoslated Rhizomucor miehei aspartic protease and other proteins, and (b) subjecting a quantity of the fermentation product to a deglycosylating treatment to form a coagulant preparation having an at least partly deglycoslated aspartic protease and the other proteins. The at least partly deglycosylated protease has a milk clotting activity that is at least 10% higher than a milk clotting activity of the glycosylated aspartic protease.

    摘要翻译: PCT No.PCT / DK95 / 00511 Sec。 371日期1997年8月14日 102(e)日期1997年8月14日PCT 1995年12月20日PCT公布。 第WO96 / 19582号公报 日期:1996年6月27日一种生产乳凝固酶的方法,包括以下步骤:(a)发酵米色真菌或米曲霉菌株以形成具有糖化的黑麦草天竺葵天冬氨酸蛋白酶等发酵产物,(b) 一定量的发酵产物进行去糖基化处理以形成具有至少部分去糖化天冬氨酸蛋白酶和其它蛋白质的凝结剂制剂。 所述至少部分去糖基化的蛋白酶具有比糖基化天冬氨酸蛋白酶的乳凝固活性高至少10%的凝乳活性。

    Rennin from recombinant microbial cells for preparation of cheese
    9.
    发明授权
    Rennin from recombinant microbial cells for preparation of cheese 失效
    来自重组微生物细胞的Rennin用于制备奶酪

    公开(公告)号:US4935354A

    公开(公告)日:1990-06-19

    申请号:US927778

    申请日:1986-11-05

    摘要: Rennin for making cheese is obtained from insoluble refractile bodies of a recombinant microbial host cell. The rennin is obtained by rupturing the recombinant host cell, isolating and solubilizing the insoluble refractile bodies, and recovering active rennin. Recombinant techniques involve preparing cDNA corresponding to the coding sequence for calf rennin, introducing into an expression vector and expressing in a host cell. As much as 200 mg rennin per liter of culture may be recovered. Prorennin or preprorennin may be produced and rennin derived therefrom.

    摘要翻译: 用于制作奶酪的Rennin由重组微生物宿主细胞的不溶性折射体获得。 通过破坏重组宿主细胞,分离和溶解不溶性折射体,并回收活性肾素获得肾素。 重组技术涉及制备对应于小牛肾素编码序列的cDNA,引入表达载体并在宿主细胞中表达。 每升培养物可以回收多达200毫克的肾素。 可以产生丙氨宁或前肾上腺素,并从中衍生肾素。

    Acylation of Mucor pusillus microbial rennet enzyme
    10.
    发明授权
    Acylation of Mucor pusillus microbial rennet enzyme 失效
    毛霉菌微生物凝乳酶的酰化

    公开(公告)号:US4362818A

    公开(公告)日:1982-12-07

    申请号:US235103

    申请日:1981-02-17

    IPC分类号: A23C19/032 C12N9/58 C12N9/96

    CPC分类号: C12N9/96 A23C19/0326 C12N9/58

    摘要: A method for increasing the milk coagulating activity of microbial rennet obtained from Mucor pusillus microorganisms. The method involves acylating Mucor pusillus microbial rennet enzyme with maleic, citraconic, phthalic, cis-1,2-cyclohexanedicarboxylic, 1,2,4-benzenetricarboxylic, homophthalic, 3-nitro-phthalic, bromomaleic or dichloromaleic anhydride.

    摘要翻译: 一种增加从毛霉微生物获得的微生物凝乳酶的凝乳活性的方法。 该方法包括用马来酸,柠康酸,邻苯二甲酸,顺式-1,2-环己烷二羧酸,1,2,4-苯三酸,间苯二甲酸,3-硝基 - 邻苯二甲酸,溴代马来酸或二氯马来酸酐酰化霉菌微生物凝乳酶。