公开(公告)号：US20180156783A1

公开(公告)日：2018-06-07

申请号：US15577809

申请日：2016-06-02

Applicant: STC.UNM

Inventor： Charles MELANCON ,  Shijie HUANG

IPC: G01N33/50 ,  C12Q1/6897 ,  G01N33/533

CPC classification number: G01N33/5076 ,  C12Q1/6897 ,  G01N33/533

Abstract: This disclosure describes a cell genetically modified to detect ribosome inhibition in the cell and methods involving such a cell. Generally, the genetically-modified cell includes an aminoglycoside-sensitive orthogonal 16S rRNA (O-16S) coding region bearing a mutated anti-Shine-Dalgarno (O-ASD) sequence, a repressor/operator system, and a polynucleotide encoding a detectable reporter under transcriptional control of the repressor/operator system. The repressor/operator system includes a coding region that encodes a transcriptional regulator and having an orthogonal SD (O-SD) sequence complementary to the 16S rRNA O-ASD sequence. The operator sequence, which is repressable by the transcriptional regulator, is operably linked to the polynucleotide encoding a detectable reporter.

公开(公告)号：US20180050050A1

公开(公告)日：2018-02-22

申请号：US15552541

申请日：2016-02-23

Applicant: Charles Melancon ,  Pamela Hall ,  Jacob Greenberg ,  Yasushi Ogasawara ,  Snezna Rogeli ,  STC.UNM

Inventor： Charles MELANCON ,  Pamela HALL ,  Jacob GREENBERG ,  Yasushi OGASAWARA ,  Snezna ROGELJ

IPC: A61K31/65

CPC classification number: A61K31/65 ,  A61K31/122 ,  A61K31/192

Abstract: In one aspect, this disclosure describes a pharmaceutical composition that generally includes frankiamicin A and a pharmaceutically acceptable carrier. In another aspect, this disclosure describes a method of treating a subject having, or at risk of having, a condition caused by a microbial infection treatable with frankiamicin A. Generally, the method includes administering to the subject an amount of frankiamicin A effective to ameliorate at least one symptom or clinical sign of the condition.