公开(公告)号：US20180156783A1

公开(公告)日：2018-06-07

申请号：US15577809

申请日：2016-06-02

Applicant: STC.UNM

Inventor： Charles MELANCON ,  Shijie HUANG

IPC: G01N33/50 ,  C12Q1/6897 ,  G01N33/533

CPC classification number: G01N33/5076 ,  C12Q1/6897 ,  G01N33/533

Abstract: This disclosure describes a cell genetically modified to detect ribosome inhibition in the cell and methods involving such a cell. Generally, the genetically-modified cell includes an aminoglycoside-sensitive orthogonal 16S rRNA (O-16S) coding region bearing a mutated anti-Shine-Dalgarno (O-ASD) sequence, a repressor/operator system, and a polynucleotide encoding a detectable reporter under transcriptional control of the repressor/operator system. The repressor/operator system includes a coding region that encodes a transcriptional regulator and having an orthogonal SD (O-SD) sequence complementary to the 16S rRNA O-ASD sequence. The operator sequence, which is repressable by the transcriptional regulator, is operably linked to the polynucleotide encoding a detectable reporter.