公开(公告)号：US20190256827A1

公开(公告)日：2019-08-22

申请号：US16227279

申请日：2018-12-20

Applicant: STC.UNM

Inventor： Fu-Sen Liang ,  Tingjun Chen ,  Dan Gao

IPC: C12N9/10 ,  C12N9/22 ,  C12N15/11 ,  C12N15/85

Abstract: An epigenome editing system generally includes three exogenous coding regions. The first exogenous coding region encodes a first polypeptide that includes an epigenetic editing domain and a domain that, in the presence of an inducer, forms a complex with a second polypeptide. The second exogenous coding region encodes the second polypeptide, which includes a dCas9 domain and a domain that, in the presence of the inducer, forms a complex with the first polypeptide. The third exogenous coding region encodes an sgRNA sequence that targets the dCas9 domain to a genomic locus.