公开(公告)号：US20210147912A1

公开(公告)日：2021-05-20

申请号：US17159028

申请日：2021-01-26

Applicant: STMICROELECTRONICS S.r.l.

Inventor： Lucio RENNA ,  Clelia Carmen GALATI ,  Natalia Maria Rita SPINELLA

IPC: C12Q1/686 ,  C12Q1/6816 ,  C12Q1/6844 ,  C12Q1/6851 ,  B01L7/00 ,  C12Q1/6825

Abstract: A method for real-time quantitative detection of single-type, target nucleic acid sequences amplified using a PCR in a microwell, comprising introducing in the microwell a sample comprising target nucleic acid sequences, magnetic primers, and labelling probes; performing an amplification cycle to form labelled amplicons; attracting the magnetic primers to a surface through a magnetic field to form a layer including labelled amplification products and free magnetic primers; and detecting the labelled amplification products in the layer with a surface-specific reading method.