公开(公告)号：US11346838B2

公开(公告)日：2022-05-31

申请号：US16522063

申请日：2019-07-25

Applicant: SYSMEX CORPORATION

Inventor： Kazuya Matsumoto ,  Takuya Iino ,  Keiko Yoshikawa

IPC: G01N33/541 ,  G01N33/536 ,  G01N33/68 ,  G01N33/53 ,  G01N33/543 ,  G01N33/569 ,  G01N33/49 ,  G01N15/14

Abstract: The present disclosure relates to a bioparticle measuring method including forming on a solid phase a complex of a sample containing a bioparticle sampled from a specimen, a capturer containing a tag which binds to the solid phase and capable of binding to the bioparticle, and a detector capable of binding to the bioparticle and containing a labeled substance. A part or the whole of the complex may be dissociated from the solid phase to prepare a measurement sample containing a part or the whole of the complex not fixed on the solid phase, and signals from the measurement sample may be detected by a particle analyzer.

公开(公告)号：US11592440B2

公开(公告)日：2023-02-28

申请号：US16522026

申请日：2019-07-25

Applicant: SYSMEX CORPORATION

Inventor： Takuya Iino ,  Keiko Yoshikawa ,  Kazuya Matsumoto

IPC: G01N33/536 ,  G01N33/49 ,  G01N33/53 ,  G01N33/543 ,  G01N33/569 ,  G01N33/541 ,  G01N15/14 ,  G01N33/68

Abstract: The present disclosure relates to a bioparticle measuring method including detecting a signal from a first measurement sample and a signal from a second measurement sample, wherein the first measurement sample is prepared by mixing a first sample containing a bioparticle sampled from a specimen with a detector capable of binding to the bioparticle and containing a labeled substance, in the presence of an inhibitor capable of binding to the bioparticle and containing none of the labeled substance. The second measurement sample is prepared by mixing a second sample sampled from the same specimen independently from the first sample with the detector, under a condition that the inhibitor is substantially absent. A measurement result is then calculated from the detected signals from the first and second measurement samples.

公开(公告)号：US11525824B2

公开(公告)日：2022-12-13

申请号：US16522063

申请日：2019-07-25

Applicant: SYSMEX CORPORATION

Inventor： Kazuya Matsumoto ,  Takuya Iino ,  Keiko Yoshikawa

IPC: G01N33/541 ,  G01N33/536 ,  G01N33/68 ,  G01N33/53 ,  G01N33/543 ,  G01N33/569 ,  G01N33/49 ,  G01N15/14

Abstract: The present disclosure relates to a bioparticle measuring method including forming on a solid phase a complex of a sample containing a bioparticle sampled from a specimen, a capturer containing a tag which binds to the solid phase and capable of binding to the bioparticle, and a detector capable of binding to the bioparticle and containing a labeled substance. A part or the whole of the complex may be dissociated from the solid phase to prepare a measurement sample containing a part or the whole of the complex not fixed on the solid phase, and signals from the measurement sample may be detected by a particle analyzer.

公开(公告)号：US20210405068A1

公开(公告)日：2021-12-30

申请号：US17357209

申请日：2021-06-24

Applicant: SYSMEX CORPORATION

Inventor： Takuya Iino ,  Shunsuke Watanabe ,  Kouzou Suto

IPC: G01N33/68

Abstract: Disclosed is a method for measuring an Aβ peptide, comprising: mixing a blood sample comprising an Aβ peptide and an antibody that specifically binds to the Aβ peptide to form a complex of the Aβ peptide and the antibody; releasing the AP peptide from the complex with a basic solution comprising an organic solvent; and measuring the released Aβ peptide by mass spectrometry.