公开(公告)号：US09803238B1

公开(公告)日：2017-10-31

申请号：US14090040

申请日：2013-11-26

Applicant: Sandia Corporation

Inventor： Chung-Yan Koh ,  Yooli Kim Light ,  Matthew Ernest Piccini ,  Anup K. Singh

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  B01L3/5027

Abstract: Embodiments of the present invention are directed toward devices, systems, and methods for purifying nucleic acids to conduct polymerase chain reaction (PCR) assays. In one example, a method includes generating complexes of silica beads and nucleic acids in a lysis buffer, transporting the complexes through an immiscible fluid to remove interfering compounds from the complexes, further transporting the complexes into a density medium containing components required for PCR where the nucleic acids disassociate from the silica beads, and thermocycling the contents of the density medium to achieve PCR. Signal may be detected from labeling agents in the components required for PCR.