公开(公告)号：US20190195865A9

公开(公告)日：2019-06-27

申请号：US15056870

申请日：2016-02-29

Applicant: Sandia Corporation

Inventor： Chung-Yan Koh ,  Ulrich Y. Schaff ,  Gregory Jon Sommer

IPC: G01N33/53

CPC classification number: G01N33/5304 ,  G01N33/54306 ,  G01N2333/245 ,  G01N2333/31 ,  G01N2333/70539 ,  G01N2333/924

Abstract: Embodiments of the present invention are directed toward devices, system and method for conducting toxin activity assay using sedimentation. The toxin activity assay may include generating complexes which bind to a plurality of beads in a fluid sample. The complexes may include a target toxin and a labeling agent, or may be generated due to presence of active target toxin and/or labeling agent designed to be incorporated into complexes responsive to the presence of target active toxin. The plurality of beads including the complexes may be transported through a density media, wherein the density media has a lower density than a density of the beads and higher than a density of the fluid sample, and wherein the transporting occurs, at least in part, by sedimentation. Signal may be detected from the labeling agents of the complexes.

公开(公告)号：US09803238B1

公开(公告)日：2017-10-31

申请号：US14090040

申请日：2013-11-26

Applicant: Sandia Corporation

Inventor： Chung-Yan Koh ,  Yooli Kim Light ,  Matthew Ernest Piccini ,  Anup K. Singh

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  B01L3/5027

Abstract: Embodiments of the present invention are directed toward devices, systems, and methods for purifying nucleic acids to conduct polymerase chain reaction (PCR) assays. In one example, a method includes generating complexes of silica beads and nucleic acids in a lysis buffer, transporting the complexes through an immiscible fluid to remove interfering compounds from the complexes, further transporting the complexes into a density medium containing components required for PCR where the nucleic acids disassociate from the silica beads, and thermocycling the contents of the density medium to achieve PCR. Signal may be detected from labeling agents in the components required for PCR.

公开(公告)号：US09304129B2

公开(公告)日：2016-04-05

申请号：US14597601

申请日：2015-01-15

Applicant: Sandia Corporation

Inventor： Ulrich Y. Schaff ,  Chung-Yan Koh ,  Gregory J. Sommer

IPC: B01L3/00 ,  G01N33/543 ,  G01N33/558 ,  G01N21/64 ,  G01N15/04 ,  B82Y15/00

CPC classification number: G01N33/54313 ,  B01L3/50215 ,  B01L3/502753 ,  B01L2200/0668 ,  B01L2200/0673 ,  B01L2400/0409 ,  B82Y15/00 ,  G01N15/04 ,  G01N21/6428 ,  G01N33/558

Abstract: Embodiments of the present invention are directed toward devices, systems, and method for conducting assays using sedimentation. In one example, a method includes layering a mixture on a density medium, subjecting sedimentation particles in the mixture to sedimentation forces to cause the sedimentation particles to move to a detection area through a density medium, and detecting a target analyte in a detection region of the sedimentation channel. In some examples, the sedimentation particles and labeling agent may have like charges to reduce non-specific binding of labeling agent and sedimentation particles. In some examples, the density medium is provided with a separation layer for stabilizing the assay during storage and operation. In some examples, the sedimentation channel may be provided with a generally flat sedimentation chamber for dispersing the particle pellet over a larger surface area.

公开(公告)号：US09304128B1

公开(公告)日：2016-04-05

申请号：US14157278

申请日：2014-01-16

Applicant: Sandia Corporation

Inventor： Chung-Yan Koh ,  Ulrich Y. Schaff ,  Gregory Jon Sommer

IPC: G01N33/53 ,  C12Q1/68 ,  G01N33/543

CPC classification number: G01N33/5304 ,  G01N33/54306 ,  G01N2333/245 ,  G01N2333/31 ,  G01N2333/70539 ,  G01N2333/924

Abstract: Embodiments of the present invention are directed toward devices, system and method for conducting toxin activity assay using sedimentation. The toxin activity assay may include generating complexes which bind to a plurality of beads in a fluid sample. The complexes may include a target toxin and a labeling agent, or may be generated due to presence of active target toxin and/or labeling agent designed to be incorporated into complexes responsive to the presence of target active toxin. The plurality of beads including the complexes may be transported through a density media, wherein the density media has a lower density than a density of the beads and higher than a density of the fluid sample, and wherein the transporting occurs, at least in part, by sedimentation. Signal may be detected from the labeling agents of the complexes.

Abstract translation: 本发明的实施方案涉及使用沉淀进行毒素活性测定的装置，系统和方法。 毒素活性测定可以包括产生结合流体样品中的多个珠子的复合物。 复合物可以包括靶毒素和标记试剂，或者由于活性目标毒素和/或被设计为掺入到复合物中的标记物的存在而产生，所述复合物对靶活性毒素的存在有响应。 包括络合物的多个珠可以通过密度介质传输，其中密度介质的密度低于珠的密度，并且高于流体样品的密度，并且其中传输至少部分地发生， 通过沉淀。 可以从复合物的标记试剂检测信号。

公开(公告)号：US20150125346A1

公开(公告)日：2015-05-07

申请号：US14597601

申请日：2015-01-15

Applicant: Sandia Corporation

Inventor： Ulrich Y. Schaff ,  Chung-Yan Koh ,  Gregory J. Sommer

IPC: G01N33/543 ,  B01L3/00 ,  G01N21/64

CPC classification number: G01N33/54313 ,  B01L3/50215 ,  B01L3/502753 ,  B01L2200/0668 ,  B01L2200/0673 ,  B01L2400/0409 ,  B82Y15/00 ,  G01N15/04 ,  G01N21/6428 ,  G01N33/558

Abstract: Embodiments of the present invention are directed toward devices, systems, and method for conducting assays using sedimentation. In one example, a method includes layering a mixture on a density medium, subjecting sedimentation particles in the mixture to sedimentation forces to cause the sedimentation particles to move to a detection area through a density medium, and detecting a target analyte in a detection region of the sedimentation channel. In some examples, the sedimentation particles and labeling agent may have like charges to reduce non-specific binding of labeling agent and sedimentation particles. In some examples, the density medium is provided with a separation layer for stabilizing the assay during storage and operation. In some examples, the sedimentation channel may be provided with a generally flat sedimentation chamber for dispersing the particle pellet over a larger surface area.

Abstract translation: 本发明的实施方案涉及用于使用沉降进行测定的装置，系统和方法。 在一个实例中，一种方法包括在密度介质上层叠混合物，使混合物中的沉降颗粒沉降，使沉淀颗粒通过密度介质移动到检测区域，并检测目标分析物在检测区域 沉淀通道。 在一些实例中，沉淀颗粒和标记剂可以具有类似的电荷以减少标记剂和沉降颗粒的非特异性结合。 在一些实例中，密度介质设置有用于在储存和操作期间稳定测定的分离层。 在一些实例中，沉淀通道可以设置有大致平坦的沉降室，用于将颗粒颗粒分散在更大的表面积上。

公开(公告)号：US20160178619A1

公开(公告)日：2016-06-23

申请号：US15056870

申请日：2016-02-29

Applicant: Sandia Corporation

Inventor： Chung-Yan Koh ,  Ulrich Y. Schaff ,  Gregory Jon Sommer

IPC: G01N33/53

CPC classification number: G01N33/5304 ,  G01N33/54306 ,  G01N2333/245 ,  G01N2333/31 ,  G01N2333/70539 ,  G01N2333/924

Abstract: Embodiments of the present invention are directed toward devices, system and method for conducting toxin activity assay using sedimentation. The toxin activity assay may include generating complexes which bind to a plurality of beads in a fluid sample. The complexes may include a target toxin and a labeling agent, or may be generated due to presence of active target toxin and/or labeling agent designed to be incorporated into complexes responsive to the presence of target active toxin. The plurality of beads including the complexes may be transported through a density media, wherein the density media has a lower density than a density of the beads and higher than a density of the fluid sample, and wherein the transporting occurs, at least in part, by sedimentation. Signal may be detected from the labeling agents of the complexes.

Abstract translation: 本发明的实施方案涉及使用沉淀进行毒素活性测定的装置，系统和方法。 毒素活性测定可以包括产生结合流体样品中的多个珠子的复合物。 复合物可以包括靶毒素和标记试剂，或者由于活性目标毒素和/或被设计为掺入到复合物中的标记物的存在而产生，所述复合物对靶活性毒素的存在有响应。 包括络合物的多个珠可以通过密度介质传输，其中密度介质的密度低于珠的密度，并且高于流体样品的密度，并且其中传输至少部分地发生， 通过沉淀。 可以从复合物的标记试剂检测信号。