公开(公告)号：US20090005255A1

公开(公告)日：2009-01-01

申请号：US11758735

申请日：2007-06-06

申请人： Sang Don JUNG ,  Myung Ae CHUNG ,  Hyo Bong HONG

发明人： Sang Don JUNG ,  Myung Ae CHUNG ,  Hyo Bong HONG

IPC分类号： C40B30/00

CPC分类号： C12Q1/6816 ,  C12Q1/6834 ,  C12Q1/6837 ,  C12Q2563/185 ,  C12Q2563/143

摘要： A method for detecting a target biological material using DNA barcodes is provided. The method comprises: attaching a first type of probes to the surface of magnetic particles to prepare magnetic particles for isolation wherein the first type of probes are at least partially complementary to a target biological material of interest; attaching a second type of probes to the surface of polymer particles to prepare polymer particles for analysis wherein the second type of probes are at least partially complementary to the target material but are different from the first type of probes, and attaching DNA barcodes as identification codes to the surface of the polymer particles wherein the DNA barcodes are present in an amount at least three times the amount of the second type of probes, have a predetermined sequence and are labeled with a label material; reacting the magnetic particles for isolation, the polymer particles for analysis and the target material in a hybridization reaction buffer to prepare composites, each of which consists of one magnetic particle for isolation, the target material and one polymer particle for analysis; separating the composites from unreacted reactants using a magnetic separator; heating the separated composites to denature the DNA barcodes present in the polymer particles for analysis and removing the magnetic particles for isolation and the polymer particles for analysis from the composites by centrifugation to isolate the DNA barcodes; and detecting a signal generated from the label material bound to the isolated DNA barcodes.

摘要翻译： 提供了使用DNA条形码检测靶生物材料的方法。 该方法包括：将第一类型的探针附着到磁性颗粒的表面以制备用于分离的磁性颗粒，其中第一类型的探针与感兴趣的目标生物材料至少部分互补; 将第二类型的探针连接到聚合物颗粒的表面以制备用于分析的聚合物颗粒，其中第二类型的探针与靶材料至少部分互补，但不同于第一类型的探针，并且附接DNA条形码作为识别代码 其中DNA条形码以至少三倍于第二类型探针的量存在的聚合物颗粒的表面具有预定的序列并用标记材料标记; 使用于分离的磁性颗粒，用于分析的聚合物颗粒和杂交反应缓冲液中的靶材料以制备复合材料，每个复合材料由用于分离的一个磁性颗粒组成，目标材料和一个用于分析的聚合物颗粒; 使用磁选机将复合材料与未反应的反应物分离; 加热分离的复合材料以使存在于聚合物颗粒中的DNA条形码变性以分析和除去用于分离的磁性颗粒和聚合物颗粒，以通过离心分离来分离DNA条形码; 并检测从与分离的DNA条形码结合的标签材料产生的信号。