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公开(公告)号:US20240253044A1
公开(公告)日:2024-08-01
申请号:US18376276
申请日:2023-10-03
申请人: 10x Genomics, Inc.
IPC分类号: B01L3/00 , B01F23/41 , B01F25/314 , B01F31/80 , B01F33/3011 , B01F33/302 , B01L3/02 , C12Q1/6806 , C12Q1/686 , C40B40/06 , G01N33/543 , G01N33/58
CPC分类号: B01L3/502784 , B01F23/41 , B01F23/4105 , B01F25/3141 , B01F25/31422 , B01F25/31423 , B01F31/84 , B01F33/3011 , B01F33/3021 , B01L3/0241 , B01L3/50273 , B01L3/502761 , C12Q1/6806 , G01N33/54313 , G01N33/58 , B01L2200/0647 , B01L2200/0652 , B01L2200/0673 , B01L2300/0851 , B01L2300/161 , B01L2400/086 , C12N2533/50 , C12Q1/686 , C12Q2563/185 , C40B40/06
摘要: Devices, systems, and their methods of use, for generating droplets are provided. One or more geometric parameters of a microfluidic channel can be selected to generate droplets of a desired and predictable droplet size.
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公开(公告)号:US20240240233A1
公开(公告)日:2024-07-18
申请号:US18389901
申请日:2023-12-20
IPC分类号: C12Q1/6806
CPC分类号: C12Q1/6806 , C12Q2521/107 , C12Q2525/161 , C12Q2543/101 , C12Q2563/179 , C12Q2563/185 , C12Q2565/514
摘要: Methods of uniquely labeling or barcoding molecules within a nucleus, a plurality of nuclei, a cell, a plurality of cells, and/or a tissue are provided. Kits for uniquely labeling or barcoding molecules within a nucleus, a plurality of nuclei, a cell, a plurality of cells, and/or a tissue are also provided. The molecules to be labeled may include, but are not limited to, RNAs and/or cDNAs.
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公开(公告)号:US20240240232A1
公开(公告)日:2024-07-18
申请号:US18389890
申请日:2023-12-20
IPC分类号: C12Q1/6806
CPC分类号: C12Q1/6806 , C12Q2521/107 , C12Q2525/161 , C12Q2543/101 , C12Q2563/179 , C12Q2563/185 , C12Q2565/514
摘要: Methods of uniquely labeling or barcoding molecules within a nucleus, a plurality of nuclei, a cell, a plurality of cells, and/or a tissue are provided. Kits for uniquely labeling or barcoding molecules within a nucleus, a plurality of nuclei, a cell, a plurality of cells, and/or a tissue are also provided. The molecules to be labeled may include, but are not limited to, RNAs and/or cDNAs.
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公开(公告)号:US12011719B2
公开(公告)日:2024-06-18
申请号:US17555013
申请日:2021-12-17
申请人: Illumina, Inc.
发明人: Arash Jamshidi , Yan-you Lin , Farnaz Absalan , Sarah Stuart , Gordon Cann , Yir-Shyuan Wu , Tarun Khurana , Jeffrey S Fisher
IPC分类号: B01L3/00 , C12Q1/6806 , G01N1/28 , G01N33/543 , C12Q1/6869 , G01N15/10
CPC分类号: B01L3/502761 , B01L3/502784 , B01L3/5088 , C12Q1/6806 , G01N1/28 , G01N33/54333 , B01L2200/0605 , B01L2200/0642 , B01L2200/0668 , B01L2300/0819 , B01L2300/165 , B01L2400/0427 , B01L2400/043 , C12Q1/6869 , C12Q2563/143 , C12Q2563/149 , C12Q2563/185 , G01N2015/1006 , C12Q1/6869 , C12Q2535/122
摘要: In accordance with embodiments herein a method for capturing cells of interest in a digital microfluidic system is provided, comprising utilizing a droplet actuator to transport a sample droplet to a microwell device. The microwell device includes a substrate having a plurality of microwells that open onto a droplet operations surface of the microwell device. The sample droplet includes cells of interest that enter the microwells. The method introduces capture beads to the microwells, and the capture elements are immobilized on the capture beads. The method utilizes the droplet actuator to transport a cell lysis reagent droplet to the microwell device. Portions of the cell lysis reagent droplet enter the microwells and, during an incubation period, cause the cells of interest to release analyte that is captured by the capture elements on the capture beads.
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公开(公告)号:US11961008B2
公开(公告)日:2024-04-16
申请号:US18050733
申请日:2022-10-28
发明人: Mark Bathe , Sakul Ratanalert , Remi Veneziano , James Banal , Tyson Shepherd
CPC分类号: G06N3/123 , B82Y10/00 , G06F12/0646 , G11C13/02 , C12Q2563/149 , C12Q2563/185 , G06F2212/251
摘要: Methods for controlled segregation of blocks of information encoded in the sequence of a biopolymer, such as nucleic acids and polypeptides, with rapid retrieval based on multiply addressing nanostructured data have been developed. In some embodiments, sequence controlled polymer memory objects include data-encoded biopolymers of any length or form encapsulated by natural or synthetic polymers and including one or more address tags. The sequence address labels are used to associate or select memory objects for sequencing read-out, enabling organization and access of distinct memory objects or subsets of memory objects using Boolean logic. In some embodiments, a memory object is a single-stranded nucleic acid scaffold strand encoding bit stream information that is folded into a nucleic acid nanostructure of arbitrary geometry, including one or more sequence address labels. Methods for controlled degradation of biopolymer-encoded blocks of information in the memory objects are also developed.
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公开(公告)号:US11959077B2
公开(公告)日:2024-04-16
申请号:US16418515
申请日:2019-05-21
IPC分类号: C12N15/10 , C12N15/113 , C12Q1/6869 , C12Q1/6876
CPC分类号: C12N15/1089 , C12N15/113 , C12Q1/6869 , C12Q1/6876 , C12Q2563/185 , C12Q2600/166
摘要: The invention relates to control compositions for sequencing and for chemical analyses, such as analytical chemistry analyses. More particularly, the invention relates to control compositions for sequencing and for chemical analyses having at least one barcode sequence fragment and at least one universal sequence fragment, and to methods of their use.
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公开(公告)号:US20240044877A1
公开(公告)日:2024-02-08
申请号:US18235616
申请日:2023-08-18
申请人: 10X GENOMICS, INC.
IPC分类号: G01N33/532 , C07K14/74 , C12N15/10 , C12N15/11 , C12N15/85 , C12Q1/6804 , C12Q1/6806 , C12Q1/6818 , C12Q1/6827 , C12Q1/6881 , G01N33/50 , G01N33/53 , G01N33/543 , G01N33/548 , G01N33/569 , G01N33/58
CPC分类号: G01N33/532 , C07K14/70539 , C12N15/1037 , C12N15/1055 , C12N15/1065 , C12N15/1075 , C12N15/11 , C12N15/85 , C12Q1/6804 , C12Q1/6806 , C12Q1/6818 , C12Q1/6827 , C12Q1/6881 , G01N33/5032 , G01N33/505 , G01N33/5304 , G01N33/5306 , G01N33/5308 , G01N33/54306 , G01N33/54366 , G01N33/548 , G01N33/56977 , G01N33/58 , C12N2310/20 , C12N2320/10 , C12Q2537/164 , C12Q2563/179 , C12Q2563/185 , C12Q2565/1015 , C40B30/04
摘要: The present disclosure provides systems and methods for processing or analyzing a sample. Methods may include generating supports (e.g., beads) comprising barcode molecules coupled thereto. A support comprising barcode molecules may be useful for analyzing or processing one or more analytes such as nucleic acid molecules, proteins, and/or perturbation agents.
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公开(公告)号:US11852628B2
公开(公告)日:2023-12-26
申请号:US18185215
申请日:2023-03-16
申请人: 10X GENOMICS, INC.
发明人: Tarjei Sigurd Mikkelsen , Eswar Prasad Ramachandran Iyer , Andrew Kohlway , Luigi Jhon Alvarado Martinez , Katherine Pfeiffer , Andrew D. Price
IPC分类号: G01N33/532 , C07K14/74 , C12N15/10 , C12N15/11 , C12N15/85 , C12Q1/6804 , C12Q1/6806 , C12Q1/6818 , C12Q1/6827 , C12Q1/6881 , G01N33/50 , G01N33/53 , G01N33/543 , G01N33/548 , G01N33/569 , G01N33/58 , C40B30/04 , C40B50/06 , C40B70/00
CPC分类号: G01N33/532 , C07K14/70539 , C12N15/1037 , C12N15/1055 , C12N15/1065 , C12N15/1075 , C12N15/11 , C12N15/85 , C12Q1/6804 , C12Q1/6806 , C12Q1/6818 , C12Q1/6827 , C12Q1/6881 , G01N33/505 , G01N33/5032 , G01N33/5304 , G01N33/5306 , G01N33/5308 , G01N33/548 , G01N33/54306 , G01N33/54366 , G01N33/56977 , G01N33/58 , C12N2310/20 , C12N2320/10 , C12Q2537/164 , C12Q2563/179 , C12Q2563/185 , C12Q2565/1015 , C40B30/04 , C40B50/06 , C40B70/00
摘要: The present disclosure provides methods of processing or analyzing a sample. A method for processing a sample may comprise hybridizing a probe molecule to a target region of a nucleic acid molecule (e.g., a ribonucleic acid (RNA) molecule), barcoding the probe-nucleic acid molecule complex, and performing extension, denaturation, and amplification processes. A method for processing a sample may comprise hybridizing first and second probes to adjacent or non-adjacent target regions of a nucleic acid molecule (e.g., an RNA molecule), linking the first and second probes to provide a probe-linked nucleic acid molecule, and barcoding the probe-linked nucleic acid molecule. One or more processes of the methods described herein may be performed within a partition, such as a droplet or well. One or more processes of the methods described herein may be performed on a cell, such as a permeabilized cell.
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公开(公告)号:US11834710B2
公开(公告)日:2023-12-05
申请号:US17136824
申请日:2020-12-29
发明人: Ronald Lebofsky , Jennifer Chew
IPC分类号: C12Q1/68 , C12Q1/6869 , C12Q1/6855 , C12Q1/6862 , C12Q1/6876 , C12Q1/6848 , C12Q1/686 , C12N15/10 , C12N9/12 , C12Q1/6806
CPC分类号: C12Q1/6869 , C12N9/1252 , C12N15/1003 , C12Q1/686 , C12Q1/6806 , C12Q1/6848 , C12Q1/6855 , C12Q1/6862 , C12Q1/6876 , C12Q2563/185 , C12Y207/07007 , C12Q1/6806 , C12Q2521/301 , C12Q2521/501 , C12Q2525/191 , C12Q2535/122 , C12Q2563/149 , C12Q2563/159 , C12Q2563/179 , C12Q2565/514
摘要: The methods and reagents are provided for barcoding and analysis of DNA samples using partition (e.g., droplet) technology while avoiding performing amplification in droplets.
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公开(公告)号:US20230304073A1
公开(公告)日:2023-09-28
申请号:US18304670
申请日:2023-04-21
IPC分类号: C12Q1/6806
CPC分类号: C12Q1/6806 , C12Q2521/107 , C12Q2525/161 , C12Q2543/101 , C12Q2563/179 , C12Q2563/185 , C12Q2565/514
摘要: Methods of uniquely labeling or barcoding molecules within a nucleus, a plurality of nuclei, a cell, a plurality of cells, and/or a tissue are provided. Kits for uniquely labeling or barcoding molecules within a nucleus, a plurality of nuclei, a cell, a plurality of cells, and/or a tissue are also provided. The molecules to be labeled may include, but are not limited to, RNAs and/or cDNAs.
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