公开(公告)号：US20240352062A1

公开(公告)日：2024-10-24

申请号：US18622013

申请日：2024-03-29

Applicant: Sanofi ,  Innate Pharma

Inventor： Thomas FRANCILLETTE ,  Benoit Mothes ,  Jérome Pezzini

IPC: C07K1/22 ,  G01N33/68

CPC classification number: C07K1/22 ,  G01N33/6857 ,  C07K2317/31 ,  C07K2317/51 ,  C07K2317/524 ,  C07K2317/526 ,  C07K2319/30

Abstract: The invention relates to a method of purifying a heteromultimeric Fc domain-containing polypeptide, wherein the method comprises the following steps in the indicated order: providing a sample comprising the heteromultimeric Fc domain-containing polypeptide and one or more mispaired variants thereof; contacting a protein A chromatography matrix with the sample and binding the heteromultimeric Fc domain-containing polypeptide to the protein A chromatography matrix; contacting the protein A chromatography matrix with a wash solution, wherein the wash solution comprises octanoate; contacting the protein A chromatography matrix with an elution solution; and collecting an eluate comprising the heteromultimeric Fc domain-containing polypeptide. The invention further relates to the use of a buffer in said method, to a kit for performing said method, and to an eluate obtained by said method.