公开(公告)号：US11661398B2

公开(公告)日：2023-05-30

申请号：US16652949

申请日：2018-10-02

申请人： Scientist of Fortune, S.A.

发明人： Tilmann Künzl ,  Sven Panke ,  Philippe Marlière

IPC分类号： C07C309/17 ,  C12N9/10

CPC分类号： C07C309/17 ,  C12N9/104 ,  C12Y203/02002

摘要： The present invention relates to a cellular transport system for bringing a sulfonic acid construct which carries a cargo into a cell and releasing the cargo in the cell's cytoplasm, the cellular transport system comprising: (i) a sulfonate transporter located in the cytoplasm membrane of the cell wherein said sulfonate transporter is capable of transporting said sulfonic acid construct across the cytoplasm membrane into the cytoplasm; (ii) a γ-glutamyl transferase (GGT; EC 2.3.2.2) which is modified to be located in the cytoplasm of the cell, wherein said γ-glutamyl transferase is capable of hydrolyzing said sulfonic acid construct so as to release the cargo. Moreover, the present invention relates to the use of a cellular transport system for bringing a sulfonic acid construct which contains a cargo into a cell and releasing the cargo in the cell's cytoplasm. Further, the present invention relates to a γ-glutamyl transferase for hydrolyzing a sulfonic acid construct which contains a cargo.