公开(公告)号：US20100112630A1

公开(公告)日：2010-05-06

申请号：US12263829

申请日：2008-11-03

申请人： Scott Martell BOYETTE ,  Hong Cai ,  Paul Ronald Hirst ,  Yan Jin ,  Juan Jiang ,  Jie Li ,  Rong Xu ,  Kechao Yang

发明人： Scott Martell BOYETTE ,  Hong Cai ,  Paul Ronald Hirst ,  Yan Jin ,  Juan Jiang ,  Jie Li ,  Rong Xu ,  Kechao Yang

IPC分类号： C12Q1/06 ,  G01N33/00

CPC分类号： G01N33/1826 ,  C12Q1/04

摘要： A process for measuring total microbiological content in an aqueous medium includes adding a fluorescent dye to the aqueous medium, measuring the fluorescent signal in the aqueous medium to obtain a baseline fluorescent signal, releasing intracellular content of the microbiological matter into the aqueous medium by lysing the microbiological matter, measuring the fluorescent signal in the aqueous medium with the released intracellular content of the microbiological matter to obtain a second fluorescent signal, subtracting the baseline signal from the second fluorescent signal to obtain a net fluorescent signal and equating the net fluorescent signal with a microbiological content. Methods for measuring biofilm and adjusting for background noise are also provided.

摘要翻译： 用于测量水性介质中的总微生物含量的方法包括向水性介质中加入荧光染料，测量水性介质中的荧光信号以获得基线荧光信号，通过裂解所述微生物物质将细胞内含量释放到水性介质中 微生物物质，测量具有微生物物质释放的细胞内含量的水性介质中的荧光信号，以获得第二荧光信号，从第二荧光信号中减去基线信号以获得净荧光信号，并将净荧光信号与 微生物含量。 还提供了测量生物膜和调整背景噪声的方法。