公开(公告)号：US06835352B2

公开(公告)日：2004-12-28

申请号：US10434214

申请日：2003-05-09

申请人： Seiichiro Ito ,  Mitsuhiro Tachibana

发明人： Seiichiro Ito ,  Mitsuhiro Tachibana

IPC分类号： B01L302

CPC分类号： B01L3/0244 ,  B01L3/0248 ,  B01L3/0262 ,  B01L2200/0605 ,  B01L2400/025 ,  B01L2400/0406 ,  Y10T436/2575

摘要： A spotting pin 10 capable of spotting equal amounts of a solution in a sequential manner comprises a first member 11 having a solution holding portion 13 formed at the tip thereof for holding a predetermined amount of solution, and a second member 12 having a solution supply portion 14 for holding the solution by a capillary action, the second member adapted to slide along the first member. As the solution supply portion 14 is brought into contact with the solution holding portion 13, the solution enters the solution holding portion 13 from the solution supply portion 14 by a capillary action. As the solution supply portion 14 and the solution holding portion 13 are separated from each other, a predetermined amount of the solution can be carried in the solution holding portion 13. Then, as the solution holding portion 13 is brought into contact with a water-absorbing support 21, a spot 22 of a predetermined amount of the solution can be formed thereon.

摘要翻译： 能够顺序地分配等量溶液的点针10包括：第一构件11，其具有形成在其顶端处的用于保持预定量溶液的溶液保持部分13;以及第二构件12，其具有溶液供应部分 14，用于通过毛细作用保持溶液，第二构件适于沿着第一构件滑动。 当溶液供应部分14与溶液保持部分13接触时，溶液通过毛细作用从溶液供应部分14进入溶液保留部分13。 当溶液供应部分14和溶液保持部分13彼此分离时，可以在溶液保留部分13中承载预定量的溶液。然后，当溶液保持部分13与水溶液保持部分13接触时， 吸收支撑件21，可以在其上形成预定量的溶液的点22。

公开(公告)号：US06726883B2

公开(公告)日：2004-04-27

申请号：US10441095

申请日：2003-05-20

申请人： Seiichiro Ito ,  Mitsuhiro Tachibana ,  Naoki Yokokawa

发明人： Seiichiro Ito ,  Mitsuhiro Tachibana ,  Naoki Yokokawa

IPC分类号： B41J225

CPC分类号： B01L3/0255 ,  B01J2219/00387 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00677 ,  B01L2400/025 ,  B82Y30/00 ,  C40B60/14 ,  Y10T436/2575

摘要： Identically shaped spots can be formed sequentially and stably by a spotting pin comprising a bar-like plunger 20. Four projections each formed in the shape of a top portion of a quadrangular pyramid are formed on the head of the plunger 20. The apexes 21 of the quadrangular pyramids constituting the projections are located inside a virtual plane extending from the peripheral wall of the plunger.

摘要翻译： 相同形状的斑点可以通过包括棒状柱塞20的斑点针顺序地且稳定地形成。在柱塞20的头部上形成四边形金字塔顶部形状的四个突起。顶点21 构成突起的四角锥体位于从柱塞的周壁延伸的虚拟平面内。

公开(公告)号：US20050158752A1

公开(公告)日：2005-07-21

申请号：US11007353

申请日：2004-12-09

申请人： Seiichiro Ito ,  Junko Tanoue ,  Fumiko Yasukawa

发明人： Seiichiro Ito ,  Junko Tanoue ,  Fumiko Yasukawa

IPC分类号： C12N15/09 ,  C12P19/34 ,  C12Q1/68

CPC分类号： C12Q1/686 ,  C12Q2527/137

摘要： A method for detecting amplified nucleic acid fragments with high accuracy is provided. The method comprises the steps of carrying out amplification reaction to amplify a predetermined region of a nucleic acid in a reaction solution comprising the nucleic acid as a template, a pair of primers, deoxynucleotides, and deoxynucleotides; hybridizing the amplified products comprised in the reaction solution to a probe; and then detecting the hybridization between the amplified products and the probe.

摘要翻译： 提供了一种高精度检测扩增核酸片段的方法。 该方法包括以下步骤：在包含核酸作为模板的反应溶液中进行扩增反应以扩增核酸的预定区域，一对引物，脱氧核苷酸和脱氧核苷酸; 将包含在反应溶液中的扩增产物与探针杂交; 然后检测扩增产物和探针之间的杂交。

公开(公告)号：US20050070028A1

公开(公告)日：2005-03-31

申请号：US10935162

申请日：2004-09-08

申请人： Seiichiro Ito ,  Junko Tanoue ,  Fumiko Yasukawa

发明人： Seiichiro Ito ,  Junko Tanoue ,  Fumiko Yasukawa

IPC分类号： G01N33/547 ,  C07K1/13 ,  C07K7/06 ,  C07K7/08 ,  G01N33/543

CPC分类号： G01N33/54353

摘要： In order to specifically and high-sensitively measure multiple antibodies, an antibody detector utilizes the specificity of an antigen-antibody reaction. The antibody detector comprises a plurality of polypeptide units, each of which is partly or entirely epitope, that are connected, with or without a spacer, and that are immobilized on a carrier.

摘要翻译： 为了特异性和高灵敏度地测量多种抗体，抗体检测器利用抗原 - 抗体反应的特异性。 抗体检测器包括多个多肽单元，每个多肽单元部分或全部是表位，其连接有或没有间隔基，并且被固定在载体上。

公开(公告)号：US07101673B2

公开(公告)日：2006-09-05

申请号：US11007353

申请日：2004-12-09

申请人： Seiichiro Ito ,  Junko Tanoue ,  Fumiko Yasukawa

发明人： Seiichiro Ito ,  Junko Tanoue ,  Fumiko Yasukawa

IPC分类号： C12Q1/68

CPC分类号： C12Q1/686 ,  C12Q2527/137

摘要： A method for detecting amplified nucleic acid fragments is provided. The method comprises the steps of carrying out amplification reaction to amplify a predetermined region of a nucleic acid in a reaction solution comprising the nucleic acid as a template, a pair of primers, deoxynucleotides, and dideoxynucleotides; hybridizing the amplified products of the reaction solution to a probe; and then detecting the hybridization between the amplified products and the probe.

摘要翻译： 提供了检测扩增的核酸片段的方法。 该方法包括以下步骤：在包含核酸作为模板的反应溶液中，进行扩增反应以扩增核酸的预定区域，一对引物，脱氧核苷酸和双脱氧核苷酸; 将反应溶液的扩增产物与探针杂交; 然后检测扩增产物和探针之间的杂交。