公开(公告)号：US06661575B1

公开(公告)日：2003-12-09

申请号：US09702337

申请日：2000-10-31

Applicant: Sergey A. Yakovenko

Inventor： Sergey A. Yakovenko

IPC: G02B2126

CPC classification number: G02B21/32

Abstract: The invention involves methods and apparata for manipulating micro-/nanoobjects. A holder exerts a weak attractive force on the micro-/nanoobject (by, for example, suction from negative fluid pressure, electrostatic attraction, magnetic attraction, etc.). A vibrator coupled to the holder generates orbital motion in the holder in at least one plane. As long as the attractive force is not too high, the orbital motion of the holder will cause rotation of the micro-/nanoobject, with the attractive force of the holder maintaining the micro-/nanoobject adjacent the holder during such rotation. Once the micro-/nanoobject has been rotated to a desired orientation, the attractive force of the holder on the micro-/nanoobject can be increased to such a level that the micro-/nanoobject will be firmly fixed to the holder. The orbital motion of the holder can be provided in conjunction with translational motion of the holder (as provided by a microdrive or similar positioning device), thereby allowing positioning of the micro-/nanoobject in as many as six degrees of freedom.

Abstract translation: 本发明涉及用于操纵微/纳米对象的方法和设备。 持有人对微/纳米对象施加弱吸引力（例如，通过例如从负流体压力的吸力，静电吸引力，磁吸引力等）。 耦合到保持器的振动器在至少一个平面中在保持器中产生轨道运动。 只要吸引力不太高，保持器的轨道运动将导致微/纳米对象的旋转，由于保持器的吸引力在这种旋转期间保持微/纳米对象与保持器相邻。 一旦微/纳米对象已经旋转到期望的方向，保持器在微/纳米对象上的吸引力可以增加到这样的水平，使得微/纳米对象将被牢固地固定到保持器。 保持器的轨道运动可以与保持器的平移运动（由微驱动器或类似定位装置提供）一起提供，从而允许将微/纳米对象定位在多达六个自由度。

公开(公告)号：US06357719B1

公开(公告)日：2002-03-19

申请号：US09596409

申请日：2000-06-19

Applicant: Sergey A. Yakovenko

Inventor： Sergey A. Yakovenko

IPC: A47G2900

CPC classification number: B01L9/50 ,  A61B34/72 ,  A61B2017/00345 ,  A61B2017/0046 ,  B01L2300/0838 ,  B25B11/002

Abstract: A microtool mount includes an elongated finger terminating in a tip. The finger has a pair of valley edges from which valley walls descend to terminate in a valley floor, thereby defining a valley between the valley walls and floor. The valley extends from the tip along a path parallel to at least a portion of the length of the finger. A microcapillary or other rodlike microtool may be accommodated within the valley, and a fastening member, preferably a magnet, may be removably affixed to the finger above the valley to maintain the microtool within the valley. The valley edges are preferably planar along at least a portion of the length of the finger so as to allow the magnet to more firmly affix to the finger.

Abstract translation: 微型工具安装件包括终止于尖端的细长指状物。 手指具有一对谷边缘，谷壁从该边缘下降到谷底，从而在谷壁和地板之间形成一个谷。 谷从尖端沿平行于手指长度的至少一部分的路径延伸。 微型毛细管或其他杆状微型工具可以容纳在谷内，并且紧固构件，优选磁体可以可移除地固定到谷上方的手指，以将微型工具保持在谷内。 谷边缘优选沿着手指长度的至少一部分是平面的，以便允许磁体更牢固地固定在手指上。

公开(公告)号：US06969604B1

公开(公告)日：2005-11-29

申请号：US10872242

申请日：2004-06-18

Applicant: Sergey A. Yakovenko

Inventor： Sergey A. Yakovenko

IPC: C12M1/42 ,  C12M3/00

CPC classification number: C12M35/02

Abstract: An electroporation chamber is provided wherein cells are borne by a fluid medium through a conductive mesh receiving electrode (with the mesh size being such that it allows passage of cells of the desired size/type) and into the chamber. The cells are then captured on a conductive mesh capturing electrode having a mesh size which retains the desired cells. The electrodes are then charged to effect electroporation or other operations within the captured cells between the electrodes. If desired, different fluid media may flow through the electrodes and chamber (and over the cells) during such activities. When operations are completed, fluid flow may be reversed to carry the treated cells back out of the chamber through the receiving electrode.

Abstract translation: 提供电穿孔室，其中细胞由流体介质通过导电网接收电极承载（网孔尺寸使得其允许所需尺寸/类型的细胞通过）并进入腔室。 然后将细胞捕获在具有保留所需细胞的网孔尺寸的导电网捕获电极上。 然后将电极充电以在电极之间的捕获的电池内进行电穿孔或其它操作。 如果需要，在这种活动期间，不同的流体介质可能流过电极和腔室（以及细胞）。 当操作完成时，流体流动可以反转，以将经处理的细胞通过接收电极返回到室外。