公开(公告)号：US20070174931A1

公开(公告)日：2007-07-26

申请号：US11725805

申请日：2007-03-20

申请人： Sherri Brown ,  Tedd Elich ,  Gregory Heck ,  Ganesh Kishore ,  Eugene Logusch ,  Sherry Logusch ,  Kenneth Piller ,  Sudabathula Rao ,  Joel Ream ,  Scott Baerson

发明人： Sherri Brown ,  Tedd Elich ,  Gregory Heck ,  Ganesh Kishore ,  Eugene Logusch ,  Sherry Logusch ,  Kenneth Piller ,  Sudabathula Rao ,  Joel Ream ,  Scott Baerson

IPC分类号： A01H1/00 ,  C12Q1/68 ,  C07H21/04 ,  C12N9/10 ,  C12N15/82 ,  C12N5/04

CPC分类号： C12N9/0071 ,  C12N9/00 ,  C12N9/0069 ,  C12N15/8222 ,  C12N15/8243 ,  C12N15/8297

摘要： Methods and materials are disclosed for the inhibition and control of gibberellic acid levels. In particular, nucleic acid sequences of copalyl diphosphate synthase. 3-β hydroxylase, and 2-oxidase and additional nucleic acid sequences are disclosed. Gibberellic acid levels may be inhibited or controlled by preparation of a chimeric expression construct capable of expressing a RNA or protein product which suppresses the gibberellin biosynthetic pathway sequence, diverts substrates from the pathway or degrades pathway substrates or products. The sequence is preferably a copalyl diphosphate synthase sequence, a 3β-hydroxylase sequence, a 2-oxidase sequence, a phytoene synthase sequence, a C20-oxidase sequence, and a 2β,3β-hydroxylase sequence. Administration of a complementing agent, preferably a gibberellin or gibberellin precursor or intermediate restores bioactivity.