公开(公告)号：US20050268355A1

公开(公告)日：2005-12-01

申请号：US11169096

申请日：2005-06-28

申请人： Kenneth Gruys ,  Timothy Mitsky ,  Ganesh Kishore ,  Steven Slater ,  Stephen Padgette ,  David Stark

发明人： Kenneth Gruys ,  Timothy Mitsky ,  Ganesh Kishore ,  Steven Slater ,  Stephen Padgette ,  David Stark

IPC分类号： A01H1/00 ,  C12N9/00 ,  C12N9/04 ,  C12N9/10 ,  C12N9/88 ,  C12N15/82 ,  C12P7/62

CPC分类号： C12N9/0006 ,  C12N9/00 ,  C12N9/1029 ,  C12N9/88 ,  C12N15/8243 ,  C12N15/8251 ,  C12N15/8253 ,  C12N15/8254 ,  C12P7/625

摘要： Nucleic acid sequences, vectors, recombinant host cells, transgenic plants, and methods for their preparation are disclosed. The nucleic acid sequences encode threonine deaminase proteins that catalyze the conversion of threonine to α-ketobutyrate. One or both of the amino acids at positions 447 and 481 of the encoded proteins can be selected from groups of particular amino acids. For example, the amino acid at position 447 can be alanine, isoleucine, valine, phenylalanine, tryptophan, or methionine. The amino acid at position 481 can be alanine, isoleucine, proline, phenylalanine, tryptophan, or methionine.

摘要翻译： 公开了核酸序列，载体，重组宿主细胞，转基因植物及其制备方法。 核酸序列编码苏氨酸脱氨酶蛋白，其催化苏氨酸转化为α-酮丁酸酯。 所编码的蛋白质的447位和481位氨基酸中的一个或两个可选自特定氨基酸的组。 例如，447位氨基酸可以是丙氨酸，异亮氨酸，缬氨酸，苯丙氨酸，色氨酸或甲硫氨酸。 位置481处的氨基酸可以是丙氨酸，异亮氨酸，脯氨酸，苯丙氨酸，色氨酸或甲硫氨酸。

公开(公告)号：US20050086713A1

公开(公告)日：2005-04-21

申请号：US10862907

申请日：2004-06-07

申请人： Balasulojini Karunanandaa ,  Martha Post-Beittenmiller ,  Mylavarapu Venkatramesh ,  Ganesh Kishore ,  Gregory Thorne ,  John LeDeaux

发明人： Balasulojini Karunanandaa ,  Martha Post-Beittenmiller ,  Mylavarapu Venkatramesh ,  Ganesh Kishore ,  Gregory Thorne ,  John LeDeaux

IPC分类号： C12N9/02 ,  C12N9/04 ,  C12N9/10 ,  C12N15/82 ,  A01H1/00 ,  C12N5/04

CPC分类号： C12N9/0073 ,  C12N9/0006 ,  C12N9/0083 ,  C12N9/1007 ,  C12N15/8243 ,  C12N15/8247 ,  C12Y101/01034 ,  C12Y114/99007

摘要： Disclosed are constructs comprising sequences encoding 3-hydroxy-3methylglutaryl-Coenzyme A reductase and at least one other sterol synthesis pathway enzyme. Also disclosed are methods for using such constructs to alter sterol production and content in cells, plants, seeds and storage organs of plants. Also provided are oils and compositions containing altered sterol levels produced by use of the disclosed constructs. Novel nucleotide sequences useful in the alteration of sterol production are also provided. Also provided are cells, plants, seeds and storage organs of plants comprising sequences encoding 3-hydroxy-3methylglutaryl-Coenzyme A reductase, at least one other sterol synthesis pathway enzyme and at least one tocopherol synthesis enzyme.

摘要翻译： 公开了包含编码3-羟基-3甲基戊二酰辅酶A还原酶和至少一种其它甾醇合成途径酶的序列的构建体。 还公开了使用这种构建体来改变植物的细胞，植物，种子和储存器官中的固醇产生和含量的方法。 还提供了含有通过使用所公开的构建体产生的改变的固醇水平的油和组合物。 还提供了可用于改变甾醇生产的新型核苷酸序列。 还提供了包含编码3-羟基-3甲基戊二酰辅酶A还原酶，至少一种其它甾醇合成途径酶和至少一种生育酚合成酶的序列的植物的细胞，植物，种子和储存器官。

公开(公告)号：US20070143878A1

公开(公告)日：2007-06-21

申请号：US11329160

申请日：2006-01-11

申请人： Barkur Bhat ,  Sekhar Boddupalli ,  Ganesh Kishore ,  Jingdong Liu ,  Shaukat Rangwala ,  Mylavarapu Venkatramesh

发明人： Barkur Bhat ,  Sekhar Boddupalli ,  Ganesh Kishore ,  Jingdong Liu ,  Shaukat Rangwala ,  Mylavarapu Venkatramesh

IPC分类号： A01H1/00 ,  C12Q1/68 ,  C07H21/04 ,  C12N9/10 ,  C07K16/40

CPC分类号： C12N15/8243 ,  C12N9/0069 ,  C12N15/8247 ,  C12Q1/6895 ,  C12Q2600/156

摘要： The present invention is in the field of plant biochemistry. More specifically the invention relates to nucleic acid sequences from plant cells, in particular, nucleic acid sequences from maize and soybean associated with the tocopherol synthesis pathway enzymes. The invention encompasses nucleic acid molecules that encode proteins and fragments of proteins. In addition, the invention also encompasses proteins and fragments of proteins so encoded and antibodies capable of binding these proteins or fragments. The invention also relates to methods of using the nucleic acid molecules, proteins and fragments of proteins and antibodies, for example for genome mapping, gene identification and analysis, plant breeding, preparation of constructs for use in plant gene expression and transgenic plants.

摘要翻译： 本发明在植物生物化学领域。 更具体地，本发明涉及来自植物细胞的核酸序列，特别是与生育酚合成途径酶相关的玉米和大豆的核酸序列。 本发明包括编码蛋白质和蛋白质片段的核酸分子。 此外，本发明还包括如此编码的蛋白质和蛋白质的片段以及能够结合这些蛋白质或片段的抗体。 本发明还涉及使用核酸分子，蛋白质和蛋白质和抗体片段的方法，例如用于基因组图谱，基因鉴定和分析，植物育种，用于植物基因表达的构建体的制备和转基因植物。

公开(公告)号：US20050102716A1

公开(公告)日：2005-05-12

申请号：US10647517

申请日：2004-01-16

申请人： Mylavarapu Venkatramesh ,  David Corbin ,  B. Bhat ,  Sekhar Boddupalli ,  Robert Grebenok ,  Ganesh Kishore ,  Kathryn Lardizabal ,  Michael Lassner ,  Shaukat Rangwala ,  Balsulojini Karunanandaa

发明人： Mylavarapu Venkatramesh ,  David Corbin ,  B. Bhat ,  Sekhar Boddupalli ,  Robert Grebenok ,  Ganesh Kishore ,  Kathryn Lardizabal ,  Michael Lassner ,  Shaukat Rangwala ,  Balsulojini Karunanandaa

IPC分类号： C12N9/02 ,  C12N15/53 ,  C12N15/54 ,  C12N15/82 ,  A01H1/00

CPC分类号： C12N9/001 ,  C12N15/8243

摘要： Provided are recombinant constructs comprising DNA sequences encoding enzymes effective in altering the biosynthesis and accumulation of sterol compounds and tocopherols in transgenic plants. Also provided are methods of using such constructs to produce transgenic plants, seeds of which contain elevated levels of sitostanol and/or sitostanol esters, and α-tocopherol, as well as reduced levels of campesterol and campestanol and their corresponding esters. These seeds also contain the novel sterol brassicastanol. Oil obtained from seeds of such transgenic plants is also provided. This oil can be used to prepare food and pharmaceutical compositions effective in lowering the level of low density lipoprotein cholesterol in blood serum. In addition, novel DNA sequences encoding plant steroid 5α-reductases are also disclosed.

摘要翻译： 提供了包含编码有效改变转基因植物中甾醇化合物和生育酚的生物合成和积累的酶的DNA序列的重组构建体。 还提供了使用这种构建体产生转基因植物的方法，其种子含有升高水平的谷甾烷醇和/或谷甾烷醇酯和α-生育酚，以及降低水平的菜油甾醇和菜油甾醇及其相应的酯。 这些种子还含有新的甾醇黄豆糖醇。 还提供了从这种转基因植物的种子获得的油。 该油可用于制备有效降低血清中低密度脂蛋白胆固醇水平的食品和药物组合物。 此外，还公开了编码植物类固醇5α-还原酶的新型DNA序列。

公开(公告)号：US20070174931A1

公开(公告)日：2007-07-26

申请号：US11725805

申请日：2007-03-20

申请人： Sherri Brown ,  Tedd Elich ,  Gregory Heck ,  Ganesh Kishore ,  Eugene Logusch ,  Sherry Logusch ,  Kenneth Piller ,  Sudabathula Rao ,  Joel Ream ,  Scott Baerson

发明人： Sherri Brown ,  Tedd Elich ,  Gregory Heck ,  Ganesh Kishore ,  Eugene Logusch ,  Sherry Logusch ,  Kenneth Piller ,  Sudabathula Rao ,  Joel Ream ,  Scott Baerson

IPC分类号： A01H1/00 ,  C12Q1/68 ,  C07H21/04 ,  C12N9/10 ,  C12N15/82 ,  C12N5/04

CPC分类号： C12N9/0071 ,  C12N9/00 ,  C12N9/0069 ,  C12N15/8222 ,  C12N15/8243 ,  C12N15/8297

摘要： Methods and materials are disclosed for the inhibition and control of gibberellic acid levels. In particular, nucleic acid sequences of copalyl diphosphate synthase. 3-β hydroxylase, and 2-oxidase and additional nucleic acid sequences are disclosed. Gibberellic acid levels may be inhibited or controlled by preparation of a chimeric expression construct capable of expressing a RNA or protein product which suppresses the gibberellin biosynthetic pathway sequence, diverts substrates from the pathway or degrades pathway substrates or products. The sequence is preferably a copalyl diphosphate synthase sequence, a 3β-hydroxylase sequence, a 2-oxidase sequence, a phytoene synthase sequence, a C20-oxidase sequence, and a 2β,3β-hydroxylase sequence. Administration of a complementing agent, preferably a gibberellin or gibberellin precursor or intermediate restores bioactivity.

公开(公告)号：US20050120406A1

公开(公告)日：2005-06-02

申请号：US10966829

申请日：2004-10-15

申请人： Albert Boronat ,  Narciso Campos ,  Ganesh Kishore

发明人： Albert Boronat ,  Narciso Campos ,  Ganesh Kishore

IPC分类号： A01H5/00 ,  A23D9/007 ,  A23K1/16 ,  C11B3/00 ,  C11B3/12 ,  C12N1/21 ,  C12N5/10 ,  C12N9/04 ,  C12N9/10 ,  C12N9/88 ,  C12N9/90 ,  C12N15/09 ,  C12N15/53 ,  C12N15/54 ,  C12N15/82 ,  C12P17/06 ,  A01H1/00 ,  C07H21/04 ,  C12N9/02 ,  C12P21/04

CPC分类号： C12N9/0006 ,  A23D9/007 ,  A23K20/174 ,  C11B3/001 ,  C11B3/12 ,  C12N9/1085 ,  C12N9/88 ,  C12N15/8243 ,  C12N15/8247 ,  C12P17/06

摘要： Methods are provided for producing plants and seeds having altered isoprenoid content and compositions. The methods find particular use in increasing the isoprenoid levels in plants, and in providing desirable isoprenoid compositions in a host plant cell.

摘要翻译： 提供了用于产生具有改变的类异戊二烯含量和组成的植物和种子的方法。 该方法特别用于增加植物中的类异戊二烯含量，以及在宿主植物细胞中提供所需的类异戊二烯组合物。

公开(公告)号：US06723837B1

公开(公告)日：2004-04-20

申请号：US09614221

申请日：2000-07-11

申请人： Balasulojini Karunanandaa ,  Jaehyuk Yu ,  Ganesh Kishore

发明人： Balasulojini Karunanandaa ,  Jaehyuk Yu ,  Ganesh Kishore

IPC分类号： C12N1509

CPC分类号： C12N15/8243 ,  C07K14/415

摘要： This invention relates to the field of biotechnology, particularly as it pertains to a nucleic acid molecule encoding a protein associated with sterol and phytosterol synthesis and metabolism. The invention also relates to methods of detection using the nucleic acid molecule, or the encoded protein as a probe or in a microarray.

摘要翻译： 本发明涉及生物技术领域，特别是涉及编码与甾醇和植物甾醇合成和代谢相关的蛋白质的核酸分子。 本发明还涉及使用核酸分子或编码的蛋白质作为探针或微阵列进行检测的方法。