公开(公告)号：US5575895A

公开(公告)日：1996-11-19

申请号：US452774

申请日：1995-05-30

申请人： Shin Ikeda ,  Mariko Miyahara ,  Toshihiko Yoshioka ,  Shiro Nankai

发明人： Shin Ikeda ,  Mariko Miyahara ,  Toshihiko Yoshioka ,  Shiro Nankai

IPC分类号： G01N27/327 ,  C12Q1/00 ,  G01N27/26

CPC分类号： C12Q1/004 ,  Y10S435/817

摘要： A biosensor for quantification of a specific component contained in various biological samples comprises an electrically insulating base plate, an electrode system including a working electrode and a counter electrode which are provided on the electrically insulating base plate, a reaction layer formed on the electrode system including at least an oxido-reductase, and an enclosure member having a hollow space constituting a sample supplying channel on the electrically insulating base plate, wherein substantially the whole of the reaction layer is exposed to the hollow space.

摘要翻译： 用于定量各种生物样品中包含的特定成分的生物传感器包括电绝缘基板，包括设置在电绝缘基板上的工作电极和对电极的电极系统，形成在电极系统上的反应层，包括 至少一种氧化还原酶，以及具有中空空间的封闭构件，该中空空间构成电绝缘基板上的样品供应通道，其中基本上整个反应层暴露于中空空间。

公开(公告)号：US5409583A

公开(公告)日：1995-04-25

申请号：US128852

申请日：1993-09-28

申请人： Toshihiko Yoshioka ,  Satoko Fujisawa ,  Mariko Miyahara ,  Shiro Nankai

发明人： Toshihiko Yoshioka ,  Satoko Fujisawa ,  Mariko Miyahara ,  Shiro Nankai

IPC分类号： G01N27/28 ,  C12Q1/00 ,  G01N27/327 ,  G01N27/416 ,  G01N27/26

CPC分类号： C12Q1/004

摘要： The present invention provides a method for measuring concentrations of the first and second substrates in a sample liquid by using a biosensor. The biosensor has an electrically insulating substrate, an electrode system, and a reaction layer including a first enzyme, a second enzyme and an electron acceptor. The method includes the steps of measuring a response current value I.sub.1 of a predetermined period of time T.sub.1 after supplying the sample liquid including the first and second substrates to the biosensor; measuring a response current value I.sub.2 of a predetermined period of time T.sub.2 after the supply of the sample liquid; obtaining the concentration C.sub.1 of the first substrate corresponding to the current value I.sub.1 based on the previously determined relationship between the concentration of the first substrate and the response current value of the predetermined period of time T.sub.1 after the supply; obtaining a response current value I.sub.1 ' corresponding to the concentration C.sub.1 of the first substrate in the sample liquid based on the previously determined relationship between the concentration of the first substrate and the response current value of the predetermined period of time T.sub.2 after the supply; and obtaining the concentration C.sub.2 of the second substrate in the sample liquid corresponding to the current value calculated by subtracting the current value I.sub.1 ' from the current value I.sub.2 based on the previously determined relationship between the concentration of the second substrate and the response current value of the predetermined period of time T.sub.2 after the supply.

摘要翻译： 本发明提供一种通过使用生物传感器测量样品液体中的第一和第二基板的浓度的方法。 生物传感器具有电绝缘基板，电极系统和包括第一酶，第二酶和电子受体的反应层。 该方法包括以下步骤：将包括第一和第二基板的样品液体供应到生物传感器之后，测量预定时间段T1的响应电流值I1; 在供给样品液体之后测量预定时间段T2的响应电流值I2; 基于先前确定的第一基板的浓度与供给后的预定时间段T1的响应电流值之间的关系，获得对应于电流值I1的第一基板的浓度C1; 基于先前确定的第一基板的浓度与供给后的预定时间段T2的响应电流值之间的关系，获得与样品液体中的第一基板的浓度C1相对应的响应电流值I1' 并且基于先前确定的第二基板的浓度与第二基板的响应电流值之间的关系，获得对应于通过从当前值I2减去电流值I1'而计算出的当前值的样品液体中的第二基板的浓度C2 在供给之后的预定时间段T2。

公开(公告)号：US5658443A

公开(公告)日：1997-08-19

申请号：US277556

申请日：1994-07-19

申请人： Tomohiro Yamamoto ,  Mariko Miyahara ,  Toshihiko Yoshioka ,  Satoko Fujisawa ,  Shiro Nankai

发明人： Tomohiro Yamamoto ,  Mariko Miyahara ,  Toshihiko Yoshioka ,  Satoko Fujisawa ,  Shiro Nankai

IPC分类号： C12Q1/00 ,  G01N27/26

CPC分类号： C12Q1/002 ,  C12Q1/001 ,  Y10S435/817

摘要： A biosensor for rapid quantification of a specific component contained in various biological samples with high accuracy has an electrically insulating base, an electrode system including a working electrode and a counter electrode formed on one face of the insulating base, and a reaction layer formed on the insulating base in close contact with the electrode system. The reaction layer contains at least a hydrophilic polymer, a buffer and an enzyme which is separated from the buffer.

摘要翻译： 用于以高精度快速定量包含在各种生物样品中的特定成分的生物传感器具有电绝缘基底，包括形成在绝缘基底的一个面上的工作电极和对电极的电极系统，以及形成在绝缘基底上的反应层 绝缘基座与电极系统紧密接触。 反应层至少含有亲水性聚合物，缓冲液和与缓冲液分离的酶。