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公开(公告)号:US5229290A
公开(公告)日:1993-07-20
申请号:US869832
申请日:1992-04-16
CPC分类号: C12N5/0025 , A01H4/008
摘要: A method is provided for the regeneration of an individual coconut plantlet. It comprises culturing a tissue explant of an undifferentiated male flower of a coconut tree in a culture medium, which contains 10-50 ppm of an auxin, 0.1-3% of a polysaccharide produced by a bacterium of Pseudomonas and 0.05-1% of activated carbon, in a dark place until a shoot-like structure appears; and then culturing under exposure to light of 3,000-20,000 luxes while repeating its subculture in a fresh culture medium.
摘要翻译: 提供了一种用于个体椰子苗的再生的方法。 其包括在培养基中培养椰子树的未分化雄花的组织外植体,其含有10-50ppm的生长素,0.1-3%的由假单胞菌细菌产生的多糖和0.05-1%的活化的 碳,在黑暗的地方,直到出现类似的结构; 然后在曝光3000-2000勒克斯的光下进行培养,同时在新鲜培养基中重复其传代培养。