-
公开(公告)号：US09447408B2
公开(公告)日：2016-09-20
申请号：US13822906
申请日：2011-09-14
CPC分类号： C12N15/00 , C12N5/0696 , C12N15/01 , C12N2501/998
摘要： The present invention provides a method of improving the efficiency of establishment of induced pluripotent stem (iPS) cells by inhibiting p38 function in the step of somatic cell nuclear reprogramming. The 38 function can be inhibited by bringing an inhibitor selected from the group consisting of (1) a chemical inhibitor of p38 (2) a dominant negative mutant of p38 or a nucleic acid that encodes the same, (3) a nucleic acid selected from the group consisting of siRNAs and shRNAs targeted to p38 and DNAs that encode the same and (4) an inhibitor of p38 pathway into contact with a somatic cell and the like. The present invention also provides an agent for improving the efficiency of establishment of induced pluripotent stem cells, which contains an inhibitor of p38 function, particularly an inhibitor selected from the group consisting of (1) a chemical inhibitor of p38 (2) a dominant negative mutant of p38 or a nucleic acid that encodes the same, (3) a nucleic acid selected from the group consisting of siRNAs and shRNAs targeted to p38 and DNAs that encode the same and (4) an inhibitor of p38 pathway. Moreover, the present invention provides a production method of iPS cells, which includes bringing a nuclear reprogramming substance and an inhibitor of p38 function into contact with a somatic cell.
摘要翻译： 本发明提供了通过在体细胞核重编程步骤中抑制p38功能来提高诱导多能干细胞（iPS）细胞建立效率的方法。 可以通过使选自（1）p38的化学抑制剂（2）p38的显性失活突变体或编码其的核酸的抑制剂来抑制38功能，（3）选自以下的核酸： 由针对p38的siRNA和shRNA组成的组和编码该p38的DNA和（4）p38途径的抑制剂与体细胞等接触的组。 本发明还提供了用于提高诱导多能干细胞的建立效率的试剂，其含有p38功能的抑制剂，特别是选自（1）p38（2）的显性阴性的化学抑制剂 p38的突变体或编码其的核酸，（3）选自靶向p38的siRNA和shRNA的核酸和编码其的DNA，以及（4）p38途径的抑制剂。 此外，本发明提供了iPS细胞的制备方法，其包括使核重编程物质和p38功能抑制剂与体细胞接触。
-
公开(公告)号：US20130183759A1
公开(公告)日：2013-07-18
申请号：US13822906
申请日：2011-09-14
IPC分类号： C12N15/01
CPC分类号： C12N15/00 , C12N5/0696 , C12N15/01 , C12N2501/998
摘要： The present invention provides a method of improving the efficiency of establishment of induced pluripotent stem (iPS) cells by inhibiting p38 function in the step of somatic cell nuclear reprogramming. The p38 function can be inhibited by bringing an inhibitor selected from the group consisting of (1) a chemical inhibitor of p38 (2) a dominant negative mutant of p38 or a nucleic acid that encodes the same, (3) a nucleic acid selected from the group consisting of siRNAs and shRNAs targeted to p38 and DNAs that encode the same and (4) an inhibitor of p38 pathway into contact with a somatic cell and the like. The present invention also provides an agent for improving the efficiency of establishment of induced pluripotent stem cells, which contains an inhibitor of p38 function, particularly an inhibitor selected from the group consisting of (1) a chemical inhibitor of p38 (2) a dominant negative mutant of p38 or a nucleic acid that encodes the same, (3) a nucleic acid selected from the group consisting of siRNAs and shRNAs targeted to p38 and DNAs that encode the same and (4) an inhibitor of p38 pathway. Moreover, the present invention provides a production method of iPS cells, which includes bringing a nuclear reprogramming substance and an inhibitor of p38 function into contact with a somatic cell.
摘要翻译： 本发明提供了通过在体细胞核重编程步骤中抑制p38功能来提高诱导多能干细胞（iPS）细胞建立效率的方法。 可以通过使选自下述的抑制剂来抑制p38的功能：（1）p38的化学抑制剂（2）p38的显性失活突变体或其编码的核酸，（3）选自以下的核酸： 由针对p38的siRNA和shRNA组成的组和编码该p38的DNA和（4）p38途径的抑制剂与体细胞等接触的组。 本发明还提供了用于提高诱导多能干细胞的建立效率的试剂，其含有p38功能的抑制剂，特别是选自（1）p38（2）的显性阴性的化学抑制剂 p38的突变体或编码其的核酸，（3）选自靶向p38的siRNA和shRNA的核酸和编码其的DNA，以及（4）p38途径的抑制剂。 此外，本发明提供了iPS细胞的制备方法，其包括使核重编程物质和p38功能抑制剂与体细胞接触。
-
搜索结果
2 条记录 (耗时 0.014 秒)