摘要:
An auxiliary method for identification of WZSP inbred line and its special primers are provided. The method is to test if DNA at site 1273 from 5′ end of genomic DNA SEQ ID NO: 1 in test pig is A or G, measure the test pig genotype is GG, GA or AA; GG or AA genotype is homozygote that DNA at site 1273 is G or A; GA genotype is their heterozygote; test pig of GG genotype is candidate for the WZSP inbred line; test pigs of GA and AA genotypes are not WZSP inbred line. The method can be applied to breed WZSP inbred line, can preliminarily screen all the pigs in test pig group, eliminate non WZSP inbred line, find out candidate WZSP inbred line, combine with other methods for further confirmation. The method can also be used to judge whether WZSP on market is counterfeiting or not.
摘要:
A method for auxiliary identification of WZSP inbred line and its special primers are disclosed. The method may comprise: testing whether a deoxyribose nucleotide at site 1273 from 5′ end of genomic DNA comprising SEQ ID NO: 1 in a test pig is A or G, measuring whether the test pig genotype is GG, GA or AA wherein the test pig with GG genotype is a candidate for the WZSP inbred line and the test pigs with GA genotype and AA genotype are non-WZSP inbred line. The method can be applied to breed WZSP inbred line, which can be used to preliminarily screen all the pigs in test pig group, eliminate non-WZSP inbred line, find out a candidate WZSP inbred line and make a further confirmation in combination with other methods. The method and primers can also be used to judge whether a WZSP on market is counterfeiting or not.
摘要翻译:公开了一种WZSP近交系辅助鉴定方法及其特殊引物。 该方法可以包括:测试测试猪中包含SEQ ID NO:1的基因组DNA的5'端的位点1273处的脱氧核糖核苷酸是否为A或G,测量测试猪基因型是GG,GA还是AA,其中测试 具有GG基因型的猪是WZSP近交系的候选者,具有GA基因型和AA基因型的测试猪是非WZSP近交系。 该方法可应用于育种WZSP近交系,可用于预先筛选试猪群中的所有猪,消除非WZSP近交系,找出候选WZSP近交系,并结合其他方法进一步确认 。 该方法和引物也可用于判断市场上的WZSP是否是假冒的。