公开(公告)号：US07374767B2

公开(公告)日：2008-05-20

申请号：US10499458

申请日：2002-12-04

申请人： Silvio Ernesto Perea Rodriguez ,  Osvaldo Reyes Acosta ,  Nelson Francisco Santiago Vispo ,  Yaquelin Puchades Izaguirre ,  Ricardo Silva Rodriguez ,  Alejandro Moro Soria ,  Alicia Santos Savio ,  Luis Javier González López ,  Belkis González Barrios

发明人： Silvio Ernesto Perea Rodriguez ,  Osvaldo Reyes Acosta ,  Nelson Francisco Santiago Vispo ,  Yaquelin Puchades Izaguirre ,  Ricardo Silva Rodriguez ,  Alejandro Moro Soria ,  Alicia Santos Savio ,  Luis Javier González López ,  Belkis González Barrios

IPC分类号： A61K39/12

CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K48/00 ,  C07K7/06 ,  C07K2319/10 ,  C12N7/00 ,  C12N2710/20022 ,  C12N2710/20032

摘要： This invention is related to the Molecular Pharmacology field and especially to the development of peptides useful for treating epithelial tumors and mainly those associated to oncogenic types of HPVs. The main objective of this invention is to identify peptides whose structure permits to block the Casein Kinase II (CKII) phosphorylation domain by direct interaction with such a site. In the present invention it is shown eleven cyclic peptides with different aminoacid sequences which inhibit the CKII phosphorylation in vitro, exhibit cytotoxicity on HPV-16 transformed cells (CaSki) and also increase the sensitivity of these cells to the cytostatic effect of interferon (IFN). Furthermore, the invention relates to the use of these peptides conjugated or fused to other peptides and chemical compounds which penetrates into cells as well as with the use of both peptide and chemical mimetic molecules.

摘要翻译： 本发明涉及分子药理学领域，特别涉及可用于治疗上皮肿瘤和主要与致癌型HPV相关的肽的发展。 本发明的主要目的是鉴定其结构允许通过与这种位点的直接相互作用阻断酪蛋白激酶II（CKII）磷酸化结构域的肽。 在本发明中，显示了11种具有不同氨基酸序列的环肽，其在体外抑制CKII磷酸化，对HPV-16转化细胞（CaSki）表现出细胞毒性，并且还增加了这些细胞对干扰素（IFN）的细胞抑制作用的敏感性， 。 此外，本发明涉及这些肽与其它肽和化合物共轭或融合的用途，这些肽和化合物可以渗入细胞以及使用肽和化学模拟分子。

公开(公告)号：US20090005294A1

公开(公告)日：2009-01-01

申请号：US12099646

申请日：2008-04-08

申请人： Silvio Ernesto Perea Rodriquez ,  Osvaldo Reyes Acosta ,  Nelson Francisco Santiago Vispo ,  Yaquelin Puchades Izaguirre ,  Ricardo Silva Rodriguez ,  Alejandro Moro Soria ,  Alicia Santos Savio ,  Luis Javier Gonzalez Lopez ,  Belkis Gonzalez Barrios

发明人： Silvio Ernesto Perea Rodriquez ,  Osvaldo Reyes Acosta ,  Nelson Francisco Santiago Vispo ,  Yaquelin Puchades Izaguirre ,  Ricardo Silva Rodriguez ,  Alejandro Moro Soria ,  Alicia Santos Savio ,  Luis Javier Gonzalez Lopez ,  Belkis Gonzalez Barrios

IPC分类号： A61K38/12 ,  C07K7/64 ,  C12N15/63 ,  C07K14/00

CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K48/00 ,  C07K7/06 ,  C07K2319/10 ,  C12N7/00 ,  C12N2710/20022 ,  C12N2710/20032

摘要： This invention is related to the Molecular Pharmacology field and especially to the development of peptides useful for treating epithelial tumors and mainly those associated to oncogenic types of HPVs. The main objective of this invention is to identify peptides whose structure permits to block the Casein Kinase II (CKII) phosphorylation domain by direct interaction with such a site. In the present invention it is shown eleven cyclic peptides with different aminoacid sequences which inhibit the CKII phosphorylation in vitro, exhibit cytotoxicity on HPV-16 transformed cells (CaSki) and also increase the sensitivity of these cells to the cytostatic effect of interferon (IFN). Furthermore, the invention relates to the use of these peptides conjugated or fused to other peptides and chemical compounds which penetrates into cells as well as with the use of both peptide and chemical mimetic molecules.

摘要翻译： 本发明涉及分子药理学领域，特别涉及可用于治疗上皮肿瘤和主要与致癌型HPV相关的肽的发展。 本发明的主要目的是鉴定其结构允许通过与这种位点的直接相互作用阻断酪蛋白激酶II（CKII）磷酸化结构域的肽。 在本发明中，显示了11种具有不同氨基酸序列的环肽，其在体外抑制CKII磷酸化，对HPV-16转化细胞（CaSki）表现出细胞毒性，并且还增加了这些细胞对干扰素（IFN）的细胞抑制作用的敏感性， 。 此外，本发明涉及这些肽与其它肽和化合物共轭或融合的用途，这些肽和化合物可以渗入细胞以及使用肽和化学模拟分子。

公开(公告)号：US07947287B2

公开(公告)日：2011-05-24

申请号：US12099646

申请日：2008-04-08

申请人： Silvio Ernesto Perea Rodriquez ,  Osvaldo Reyes Acosta ,  Nelson Francisco Santiago Vispo ,  Yaquelin Puchades Izaguirre ,  Ricardo Silva Rodriguez ,  Alejandro Moro Soria ,  Alicia Santos Savio ,  Luis Javier Gonzalez Lopez ,  Belkis Gonzalez Barrios

发明人： Silvio Ernesto Perea Rodriquez ,  Osvaldo Reyes Acosta ,  Nelson Francisco Santiago Vispo ,  Yaquelin Puchades Izaguirre ,  Ricardo Silva Rodriguez ,  Alejandro Moro Soria ,  Alicia Santos Savio ,  Luis Javier Gonzalez Lopez ,  Belkis Gonzalez Barrios

IPC分类号： A61K39/12

CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K48/00 ,  C07K7/06 ,  C07K2319/10 ,  C12N7/00 ,  C12N2710/20022 ,  C12N2710/20032

摘要： This invention is related to the Molecular Pharmacology field and especially to the development of peptides useful for treating epithelial tumors and mainly those associated to oncogenic types of HPVs. The main objective of this invention is to identify peptides whose structure permits to block the Casein Kinase II (CKII) phosphorylation domain by direct interaction with such a site. In the present invention it is shown eleven cyclic peptides with different aminoacid sequences which inhibit the CKII phosphorylation in vitro, exhibit cytotoxicity on HPV-16 transformed cells (CaSki) and also increase the sensitivity of these cells to the cytostatic effect of interferon (IFN). Furthermore, the invention relates to the use of these peptides conjugated or fused to other peptides and chemical compounds which penetrates into cells as well as with the use of both peptide and chemical mimetic molecules.

摘要翻译： 本发明涉及分子药理学领域，特别涉及可用于治疗上皮肿瘤和主要与致癌型HPV相关的肽的发展。 本发明的主要目的是鉴定其结构允许通过与这种位点的直接相互作用阻断酪蛋白激酶II（CKII）磷酸化结构域的肽。 在本发明中，显示了11种具有不同氨基酸序列的环肽，其在体外抑制CKII磷酸化，对HPV-16转化细胞（CaSki）表现出细胞毒性，并且还增加了这些细胞对干扰素（IFN）的细胞抑制作用的敏感性， 。 此外，本发明涉及这些肽与其它肽和化合物共轭或融合的用途，这些肽和化合物可以渗入细胞以及使用肽和化学模拟分子。

公开(公告)号：US20060233742A1

公开(公告)日：2006-10-19

申请号：US10499458

申请日：2002-12-04

申请人： Silvio Rodriguez ,  Osvaldo Reyes Acosta ,  Nelson Santiago Vispo ,  Yaquelin Puchades Izaguirre ,  Ricardo Silva Rodriguez ,  Alejandro Moro Soria ,  Alicia Santos Savio ,  Luis González López ,  Belkis González Barrios

发明人： Silvio Rodriguez ,  Osvaldo Reyes Acosta ,  Nelson Santiago Vispo ,  Yaquelin Puchades Izaguirre ,  Ricardo Silva Rodriguez ,  Alejandro Moro Soria ,  Alicia Santos Savio ,  Luis González López ,  Belkis González Barrios

IPC分类号： A61K38/19 ,  A61K38/10 ,  A61K38/12 ,  A61K38/21

CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K48/00 ,  C07K7/06 ,  C07K2319/10 ,  C12N7/00 ,  C12N2710/20022 ,  C12N2710/20032

摘要： This invention is related to the Molecular Pharmacology field and especially to the development of peptides useful for treating epithelial tumors and mainly those associated to oncogenic types of HPVs. The main objective of this invention is to identify peptides whose structure permits to block the Casein Kinase II (CKII) phosphorylation domain by direct interaction with such a site. In the present invention it is shown eleven cyclic peptides with different aminoacid sequences which inhibit the CKII phosphorylation in vitro, exhibit cytotoxicity on HPV-16 transformed cells (CaSki) and also increase the sensitivity of these cells to the cytostatic effect of interferon (IFN). Furthermore, the invention relates to the use of these peptides conjugated or fused to other peptides and chemical compounds which penetrates into cells as well as with the use of both peptide and chemical mimetic molecules.

摘要翻译： 本发明涉及分子药理学领域，特别涉及可用于治疗上皮肿瘤和主要与致癌型HPV相关的肽的发展。 本发明的主要目的是鉴定其结构允许通过与这种位点的直接相互作用阻断酪蛋白激酶II（CKII）磷酸化结构域的肽。 在本发明中，显示了11种具有不同氨基酸序列的环肽，其在体外抑制CKII磷酸化，对HPV-16转化细胞（CaSki）表现出细胞毒性，并且还增加了这些细胞对干扰素（IFN）的细胞抑制作用的敏感性， 。 此外，本发明涉及这些肽与其它肽和化合物共轭或融合的用途，这些肽和化合物可以渗入细胞以及使用肽和化学模拟分子。

公开(公告)号：US08007776B2

公开(公告)日：2011-08-30

申请号：US10529923

申请日：2003-10-08

申请人： Alicia Santos Savio ,  Ricardo Silva Rodriguez ,  Yanelis Morera Diaz ,  Armando Alexei Rodriquez Alfonso ,  Jose Rafael Martinez Castillo ,  Haydee Geronimo Perez ,  Alejandro Moro Soria ,  Silvio Ernesto Perea Rodriguez

发明人： Alicia Santos Savio ,  Ricardo Silva Rodriguez ,  Yanelis Morera Diaz ,  Armando Alexei Rodriquez Alfonso ,  Jose Rafael Martinez Castillo ,  Haydee Geronimo Perez ,  Alejandro Moro Soria ,  Silvio Ernesto Perea Rodriguez

IPC分类号： A61K38/20

CPC分类号： A61K39/0005

摘要： Vaccine composition containing IL-15 for active immunization to treatment diseases related to over-expression of this cytokine. The technical aim pursued in this invention is to inhibit IL-15 activity through development of a therapeutic vaccine which elicits IL-15 neutralizing antibodies in the immunized host.IL-15 can be presented in this vaccine composition either alone or coupled to a carrier such as the P64k protein with a vaccine adjuvant such as Aluminum hydroxide.Besides, this invention is related to the use of this vaccine for treatment of Rheumatoid arthritis and other IL-15 over-expressing related diseases, and leukemia, where this cytokine acts as a growth factor.

摘要翻译： 含有IL-15的疫苗组合物用于主动免疫治疗与该细胞因子的过表达有关的疾病。 在本发明中追求的技术目的是通过开发在免疫宿主中引发IL-15中和抗体的治疗性疫苗来抑制IL-15活性。 IL-15可以单独存在于该疫苗组合物中，也可以与诸如氢氧化铝等疫苗佐剂的载体例如P64k蛋白偶联。 此外，本发明涉及该疫苗用于治疗类风湿性关节炎和其它IL-15过表达相关疾病和白血病，其中该细胞因子作为生长因子。