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公开(公告)号:US09353389B2
公开(公告)日:2016-05-31
申请号:US14285354
申请日:2014-05-22
Applicant: Solazyme, Inc.
Inventor: Scott Franklin , Aravind Somanchi , Karen Espina , George Rudenko , Penelope Chua
IPC: A01H13/00 , C12P7/64 , C10L1/02 , C12N9/00 , C12N9/16 , C12P7/20 , C12N15/79 , C07C1/207 , C12N9/24 , A01H1/00
CPC classification number: C12P7/6427 , C07C1/2078 , C07K2319/01 , C10L1/02 , C12N9/00 , C12N9/16 , C12N9/2402 , C12N15/79 , C12P7/20 , C12P7/64 , C12P7/6409 , C12P7/6418 , C12P7/6463 , C12P7/649 , C12Y301/02014 , C12Y302/01026 , Y02E50/13 , Y02P20/52 , Y02P60/877 , Y02T50/678
Abstract: Novel gene sequences from microalgae are disclosed, as well as novel gene sequences useful in the manufacture of triglyceride oils. Also disclosed are sequences and vectors that allow microalgae to be cultivated on sugar cane and sugar beets as a feedstock. In some embodiments, the vectors are useful for the purpose of performing targeted modifications to the nuclear genome of heterotrophic microalgae.
Abstract translation: 公开了来自微藻的新型基因序列,以及可用于制备甘油三酯油的新型基因序列。 还公开了允许将微藻种植在甘蔗和甜菜作为原料的序列和载体。 在一些实施方案中,载体可用于进行对异养微藻的核基因组的靶向修饰。
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公开(公告)号:US20150073163A1
公开(公告)日:2015-03-12
申请号:US14474238
申请日:2014-09-01
Applicant: Solazyme, Inc.
Inventor: Penelope Chua , Aravind Somanchi
CPC classification number: C12P7/6463 , C12N1/12 , C12N1/22 , C12N15/52 , C12N15/8246 , C12N15/8247 , C12P7/64 , C12P7/649 , C12P2203/00 , Y02E50/13 , Y02T50/678
Abstract: The invention provides methods of cultivating oil-bearing microbes using xylose as a fixed carbon source. Also provided are microorganisms that have been genetically engineered to metabolize xylose as a fixed carbon source allowing them to convert xylose into oils. Particular advantages of the processes provided herein include production of oils rather than alcohols through the microbial fermentation processes utilizing xylose.
Abstract translation: 本发明提供了使用木糖作为固定碳源培养含油微生物的方法。 还提供了经遗传工程化以将木糖代谢为固定碳源的微生物,从而允许木糖转化为油。 本文提供的方法的特别优点包括通过利用木糖的微生物发酵方法生产油而不是醇。
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3.
公开(公告)号:US20150218604A1
公开(公告)日:2015-08-06
申请号:US14626505
申请日:2015-02-19
Applicant: Solazyme, Inc.
Inventor: Scott Franklin , Aravind Somanchi , Karen Espina , George Rudenko , Penelope Chua
CPC classification number: C12P7/6427 , C07C1/2078 , C07K2319/01 , C10L1/02 , C12N9/00 , C12N9/16 , C12N9/2402 , C12N15/79 , C12P7/20 , C12P7/64 , C12P7/6409 , C12P7/6418 , C12P7/6463 , C12P7/649 , C12Y301/02014 , C12Y302/01026 , Y02E50/13 , Y02P20/52 , Y02P60/877 , Y02T50/678
Abstract: Disclosed herein are methods of manufacturing renewable chemicals through the manufacture of novel triglyceride oils followed by chemical modification of the oils. Methods such as transesterification, hydrogenation, hydrocracking, deoxygenation, isomerization, interesterification, hydroxylation, hydrolysis and saponification are disclosed. Novel oils containing fatty acid chain lengths of C8, C10, C12 or C14 are also disclosed and are useful as feedstocks in the methods of the invention.
Abstract translation: 本文公开了通过制备新型甘油三酯油然后化学改性油来制造可再生化学品的方法。 公开了酯交换,氢化,加氢裂化,脱氧,异构化,酯交换,羟基化,水解和皂化等方法。 还公开了含有C8,C10,C12或C14脂肪酸链长度的新型油,并且可用作本发明方法中的原料。
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公开(公告)号:US08951777B2
公开(公告)日:2015-02-10
申请号:US13852116
申请日:2013-03-28
Applicant: Solazyme, Inc.
Inventor: Scott Franklin , Aravind Somanchi , Karen Espina , George Rudenko , Penelope Chua
IPC: C12N1/12 , C12N15/82 , C12N9/24 , C12P7/64 , C10L1/02 , C12N9/00 , C12N9/16 , C12P7/20 , C12N15/79 , C07C1/207
CPC classification number: C12P7/6427 , C07C1/2078 , C07K2319/01 , C10L1/02 , C12N9/00 , C12N9/16 , C12N9/2402 , C12N15/79 , C12P7/20 , C12P7/64 , C12P7/6409 , C12P7/6418 , C12P7/6463 , C12P7/649 , C12Y301/02014 , C12Y302/01026 , Y02E50/13 , Y02P20/52 , Y02P60/877 , Y02T50/678
Abstract: Disclosed herein are obligate heterotrophic microalgae cells containing an exogenous gene. In some embodiments the gene is a sucrose utilization gene, and further disclosed are methods of manufacturing triglyceride oils using sugar cane or sugar beets as a feedstock in a heterotrophic fermentation. In other embodiments the feedstock is depolymerized cellulosic material. Also disclosed are cells that produce medium chain fatty acids at levels not produced in non-recombinant cells of the same species and genus.
Abstract translation: 本文公开了含有外源基因的专性异养微藻细胞。 在一些实施方案中,该基因是蔗糖利用基因,并且进一步公开了使用甘蔗或甜菜作为异养发酵中的原料来制备甘油三酯油的方法。 在其它实施方案中,原料是解聚的纤维素材料。 还公开了以相同物种和属的非重组细胞中不产生的水平产生中链脂肪酸的细胞。
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公开(公告)号:US20140256024A1
公开(公告)日:2014-09-11
申请号:US14285354
申请日:2014-05-22
Applicant: Solazyme, Inc.
Inventor: Scott Franklin , Aravind Somanchi , Karen Espina , George Rudenko , Penelope Chua
IPC: C12N9/24
CPC classification number: C12P7/6427 , C07C1/2078 , C07K2319/01 , C10L1/02 , C12N9/00 , C12N9/16 , C12N9/2402 , C12N15/79 , C12P7/20 , C12P7/64 , C12P7/6409 , C12P7/6418 , C12P7/6463 , C12P7/649 , C12Y301/02014 , C12Y302/01026 , Y02E50/13 , Y02P20/52 , Y02P60/877 , Y02T50/678
Abstract: Novel gene sequences from microalgae are disclosed, as well as novel gene sequences useful in the manufacture of triglyceride oils. Also disclosed are sequences and vectors that allow microalgae to be cultivated on sugar cane and sugar beets as a feedstock. In some embodiments, the vectors are useful for the purpose of performing targeted modifications to the nuclear genome of heterotrophic microalgae.
Abstract translation: 公开了来自微藻的新型基因序列,以及可用于制备甘油三酯油的新型基因序列。 还公开了允许将微藻种植在甘蔗和甜菜作为原料的序列和载体。 在一些实施方案中,载体可用于进行对异养微藻的核基因组的靶向修饰。
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