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公开(公告)号：US20160194672A1
公开(公告)日：2016-07-07
申请号：US14975137
申请日：2015-12-18
Applicant: Solazyme, Inc.
Inventor： Scott Franklin , Aravind Somanchi , George Rudenko , Riyaz Bhat , Xinhua Zhao , Risha Bond , Walter Rakitsky , Alejandro Marangoni , Diza Braksmayer
IPC: C12P7/64 , C12N9/24 , C12N15/113 , C12N9/10
CPC classification number: C12P7/6409 , A23D9/00 , A23D9/013 , C07C57/03 , C07J9/00 , C11B1/00 , C12N9/0071 , C12N9/1029 , C12N9/16 , C12N9/2402 , C12N15/113 , C12N15/8247 , C12N2310/531 , C12P7/64 , C12P7/6445 , C12P7/6463 , C12P7/6472 , C12P33/00 , C12R1/89 , C12Y203/01015 , C12Y203/0102 , C12Y203/01041 , C12Y203/01051 , C12Y203/01179 , C12Y203/0118 , C12Y302/01014 , C12Y302/01026 , Y02E50/13 , Y02P20/52
Abstract: Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsatturated-saturated type.
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公开(公告)号：US20160186219A1
公开(公告)日：2016-06-30
申请号：US14974983
申请日：2015-12-18
Applicant: Solazyme, Inc.
Inventor： Scott Franklin , Aravind Somanchi , Janice Wee , George Rudenko , Jeffrey L. Moseley , Walter Rakitsky , Xinhua Zhao , Riyaz Bhat
CPC classification number: C12P7/6463 , C07C69/52 , C10L1/02 , C10L2200/0476 , C10L2290/26 , C10L2290/30 , C12N9/0071 , C12N9/1029 , C12N9/16 , C12N15/52 , Y02E50/13
Abstract: Methods and compositions for the production of oil, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty acid hydroxylase, a desaturase enzyme, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants.
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3.发明授权Genetically engineered microbial strains including Prototheca lipid pathway genes 有权基因工程微生物菌株，包括原生动物体脂通路基因公开(公告)号：US09328351B2
公开(公告)日：2016-05-03
申请号：US13688025
申请日：2012-11-28
Applicant: Solazyme, Inc.
Inventor： Scott Franklin , Shane Brubaker , George N. Rudenko , Jeffrey L. Moseley , Xinhua Zhao , Tina T. Huynh , Riyaz Bhat , Matthew Shoa-Azar , Trung H. Nguyen , Karen Espina , Aravind Somanchi
CPC classification number: A61K39/0011 , C07K14/405 , C12N5/0634 , C12N9/00 , C12N9/0071 , C12N9/1029 , C12N9/16 , C12N15/79 , C12N15/8247 , C12P7/6445 , C12P7/6463 , C12Y114/19002 , C12Y114/19006 , C12Y203/01179 , C12Y301/02014 , Y02P20/52
Abstract: Genetically engineered microbial, e.g., Prototheca, cells provide microbial oil useful as a food additive and a source of renewable fuels and industrial chemicals.
Abstract translation: 基因工程微生物，例如Prototheca，细胞提供可用作食品添加剂的微生物油和可再生燃料和工业化学品的来源。
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公开(公告)号：US09255282B2
公开(公告)日：2016-02-09
申请号：US14276943
申请日：2014-05-13
Applicant: Solazyme, Inc.
Inventor： Scott Franklin , Aravind Somanchi , Janice Wee , George Rudenko , Jeffrey L. Moseley , Walt Rakitsky
CPC classification number: C12N15/79 , A23D7/00 , A23D9/00 , A23K20/158 , C11B1/00 , C11C3/00 , C12N9/0071 , C12N9/16 , C12N9/2431 , C12N9/2465 , C12N15/67 , C12N15/8216 , C12P7/64 , C12P7/6445 , C12P7/6463 , C12Y114/19001 , C12Y301/02 , C12Y301/02014 , C12Y302/01022 , C12Y302/01026 , Y02E50/13 , Y02P30/20 , Y02T50/678
Abstract: Methods and compositions for the production of oil, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants.
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公开(公告)号：US20160010025A1
公开(公告)日：2016-01-14
申请号：US14801172
申请日：2015-07-16
Applicant: Solazyme, Inc.
Inventor： Harrison F. Dillon , Dan Elefant , Anthony G. Day , Scott Franklin , Jon Wittenberg
CPC classification number: C11B1/10 , C10L1/026 , C11B1/106 , C11C3/003 , C11C3/04 , C12P7/6463 , C12P7/649 , Y02E50/13 , Y02T50/678
Abstract: The invention generally relates to the production of hydrocarbon compositions, such as a lipid, in microorganisms. In particular, the invention provides methods for extracting, recovering, isolating and obtaining a lipid from a microorganism and compositions comprising the lipid. The invention also discloses methods for producing hydrocarbon compositions for use as biodiesel, renewable diesel, jet fuel, and other materials.
Abstract translation: 本发明一般涉及微生物中烃类组合物如脂质的生产。 特别地，本发明提供从微生物中提取，回收，分离和获得脂质以及包含脂质的组合物的方法。 本发明还公开了用于生产用作生物柴油，可再生柴油，喷气燃料和其它材料的烃组合物的方法。
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Patent Agency Ranking
公开(公告)号：US09205040B2
公开(公告)日：2015-12-08
申请号：US14015921
申请日：2013-08-30
Applicant: Solazyme, Inc.
Inventor： Geoffrey Brooks , Scott Franklin
IPC: A01N65/00 , A61K8/97 , A61K8/92 , A61Q19/00 , C12N1/12 , C12R1/89 , A61Q1/06 , A61Q5/02 , A61Q15/00 , A61Q19/10
CPC classification number: A61K8/99 , A61K8/498 , A61K8/922 , A61K8/9706 , A61K2800/10 , A61K2800/805 , A61Q1/06 , A61Q5/02 , A61Q5/12 , A61Q15/00 , A61Q19/00 , A61Q19/10 , C12N1/12 , C12R1/89
Abstract: The invention provides cosmetic compositions comprising microalgal biomass, whole microalgal cells, and/or microalgal oil in combination with one or more other cosmetic ingredients, and methods of making such compositions. In preferred embodiments, the microalgal components of the cosmetic compositions are derived from microalgal cultures grown heterotrophically and which comprise at least 10% oil by dry weight.
Abstract translation: 本发明提供包含与一种或多种其它化妆品组分组合的微藻生物质，全部微藻细胞和/或微藻油的化妆品组合物，以及制备这种组合物的方法。 在优选的实施方案中，化妆品组合物的微藻组分衍生自异养生长并由干重至少10％的油生长的微藻培养基。
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公开(公告)号：US20140275586A1
公开(公告)日：2014-09-18
申请号：US13837996
申请日：2013-03-15
Applicant: Solazyme, Inc.
Inventor： George N. Rudenko , Jason Casolari , Scott Franklin
IPC: C12P7/64
CPC classification number: C12P7/6409 , A23L33/115 , A23V2002/00 , C10L1/02 , C10L2200/0476 , C10L2290/26 , C12N9/16 , C12P7/6463 , C12Y301/02014
Abstract: The invention features plant acyl-ACP thioesterase genes of the FatB class and proteins encoded by these genes. The genes are useful for constructing recombinant host cells having altered fatty acid profiles. Oleaginous microalga host cells with the new genes or previously identified FatB genes are disclosed. The microalgae cells produce triglycerides with useful fatty acid profiles.
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公开(公告)号：US20140093945A1
公开(公告)日：2014-04-03
申请号：US14055763
申请日：2013-10-16
Applicant: Solazyme, Inc.
Inventor： Harrison F. Dillon , Dan Elefant , Anthony G. Day , Scott Franklin , Jon Wittenberg
IPC: C11B1/10
CPC classification number: C11B1/10 , C10L1/026 , C11B1/106 , C11C3/003 , C11C3/04 , C12P7/6463 , C12P7/649 , Y02E50/13 , Y02T50/678
Abstract: The invention generally relates to the production of hydrocarbon compositions, such as a lipid, in microorganisms. In particular, the invention provides methods for extracting, recovering, isolating and obtaining a lipid from a microorganism and compositions comprising the lipid. The invention also discloses methods for producing hydrocarbon compositions for use as biodiesel, renewable diesel, jet fuel, and other materials.
Abstract translation: 本发明一般涉及微生物中烃类组合物如脂质的生产。 特别地，本发明提供从微生物中提取，回收，分离和获得脂质以及包含脂质的组合物的方法。 本发明还公开了用于生产用作生物柴油，可再生柴油，喷气燃料和其它材料的烃组合物的方法。
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公开(公告)号：US20130330790A1
公开(公告)日：2013-12-12
申请号：US13849330
申请日：2013-03-22
Applicant: SOLAZYME, INC.
Inventor： Donald E. Trimbur , Chung-Soon Im , Harrison F. Dillon , Anthony G. Day , Scott Franklin , Anna Coragliotti
CPC classification number: C10L1/026 , C07H21/04 , C10L1/06 , C10L2200/0484 , C10L2270/026 , C10L2270/04 , C11C3/00 , C12N1/12 , C12N1/32 , C12N9/0006 , C12N9/0008 , C12N9/1205 , C12N9/14 , C12N9/16 , C12N9/20 , C12N9/2408 , C12N9/2431 , C12N9/88 , C12N15/79 , C12P5/02 , C12P7/6409 , C12P7/6436 , C12P7/6445 , C12P7/6463 , C12P7/649 , C12P21/06 , C12Y302/01026 , Y02E50/10 , Y02E50/13 , Y02E50/343 , Y02P20/52 , Y02T50/678
Abstract: The invention provides methods of cultivating oil-bearing microbes using xylose alone or in combination with other depolymerized cellulosic material. Also provided are microorganisms comprising an exogenous gene encoding a polysaccharide degrading enzyme, such as a cellulase, a hemicellulase, a pectinase, or a driselase. Some methods of microbial fermentation are provided that comprise the use of xylose and depolymerized cellulosic materials for the production of oil-bearing microorgansims.
Abstract translation: 本发明提供了使用单独的木糖或与其它解聚的纤维素材料组合培养含油微生物的方法。 还提供了包含编码多糖降解酶的外源基因如纤维素酶，半纤维素酶，果胶酶或者碎裂酶的微生物。 提供了微生物发酵的一些方法，其包括使用木糖和解聚的纤维素材料来生产含油微生物。
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公开(公告)号：US09353389B2
公开(公告)日：2016-05-31
申请号：US14285354
申请日：2014-05-22
Applicant: Solazyme, Inc.
Inventor： Scott Franklin , Aravind Somanchi , Karen Espina , George Rudenko , Penelope Chua
IPC: A01H13/00 , C12P7/64 , C10L1/02 , C12N9/00 , C12N9/16 , C12P7/20 , C12N15/79 , C07C1/207 , C12N9/24 , A01H1/00
CPC classification number: C12P7/6427 , C07C1/2078 , C07K2319/01 , C10L1/02 , C12N9/00 , C12N9/16 , C12N9/2402 , C12N15/79 , C12P7/20 , C12P7/64 , C12P7/6409 , C12P7/6418 , C12P7/6463 , C12P7/649 , C12Y301/02014 , C12Y302/01026 , Y02E50/13 , Y02P20/52 , Y02P60/877 , Y02T50/678
Abstract: Novel gene sequences from microalgae are disclosed, as well as novel gene sequences useful in the manufacture of triglyceride oils. Also disclosed are sequences and vectors that allow microalgae to be cultivated on sugar cane and sugar beets as a feedstock. In some embodiments, the vectors are useful for the purpose of performing targeted modifications to the nuclear genome of heterotrophic microalgae.
Abstract translation: 公开了来自微藻的新型基因序列，以及可用于制备甘油三酯油的新型基因序列。 还公开了允许将微藻种植在甘蔗和甜菜作为原料的序列和载体。 在一些实施方案中，载体可用于进行对异养微藻的核基因组的靶向修饰。
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