公开(公告)号：US20240409986A1

公开(公告)日：2024-12-12

申请号：US18268964

申请日：2022-05-06

Applicant: South China Normal University

Inventor： Xiaoming ZHOU ,  Menglu HU

IPC: C12Q1/6844 ,  C12N9/22 ,  C12N15/11

Abstract: The present invention discloses a nucleic acid detection kit and a detection method based on photo-controlled CRISPR-Cas, wherein the kit comprises silent guide RNA and Cas protein; the silent guide RNA is formed by annealing hybridization of silent nucleotide and guide RNA; the guide RNA, designed according to a target nucleic acid sequence, includes two regions, i.e. a repetitive region and a spacer region; the silent nucleotide is completely complementarily paired with a the of the guide RNA, or is completely paired with a the of the guide RNA; the bases of the silent nucleotide are linked by PC linker; and the Cas protein is Cas12 protein or Cas13 protein. Although this method separates the nucleic acid amplification from the CRISPR-Cas detection in time, it can allow them to be completed in the same closed reaction tube, thereby avoiding the transfer process of uncapped reagent, ensuring that the detection is not affected by aerosol pollution while ensuring high detection sensitivity.