公开(公告)号：US09719117B2

公开(公告)日：2017-08-01

申请号：US14132473

申请日：2013-12-18

申请人： Steffen Schaffer ,  Jasmin Gielen ,  Mirja Wessel ,  Hans-Georg Hennemann ,  Harald Haeger ,  Thomas Haas ,  Wilfried Bluemke

发明人： Steffen Schaffer ,  Jasmin Gielen ,  Mirja Wessel ,  Hans-Georg Hennemann ,  Harald Haeger ,  Thomas Haas ,  Wilfried Bluemke

IPC分类号： C12P13/00 ,  C12N9/12 ,  C12N9/02 ,  C12N9/06 ,  C12N9/10

CPC分类号： C12P13/001 ,  C12N9/0008 ,  C12N9/0016 ,  C12N9/0069 ,  C12N9/1096 ,  C12N9/1288 ,  C12P13/005 ,  Y02P20/582

摘要： The invention provides a whole cell catalyst which expresses a recombinant α-dioxygenase or the combination of a recombinant fatty acid reductase and a phosphopantetheinyl transferase phosphopantetheinylating the fatty acid reductase, and which in addition to the α-dioxygenase and/or the combination of fatty acid reductase and phosphopantetheinyl transferase expresses a transaminase, characterized in that the phosphopantetheinyl transferase and/or transaminase is preferably recombinant; and a method for the conversion of a fatty acid, ω-hydroxy fatty acid, ω-oxo fatty acid or a monoester thereof to an amine, comprising oxidation of the fatty acid, ω-hydroxy fatty acid, ω-oxo fatty acid or the monoester thereof to an oxidation product by contacting with an alkane hydroxylase and/or alcohol dehydrogenase, contacting the oxidation product with a phosphopantetheinylated fatty acid reductase or a α-dioxygenase to give an aldehyde, and contacting the aldehyde with a transaminase.

公开(公告)号：US20140178948A1

公开(公告)日：2014-06-26

申请号：US14132473

申请日：2013-12-18

申请人： Steffen SCHAFFER ,  Jasmin Gielen ,  Mirja Wessel ,  Hans-Georg Hennemann ,  Harald Haeger ,  Thomas Haas ,  Wilfried Bluemke

发明人： Steffen SCHAFFER ,  Jasmin Gielen ,  Mirja Wessel ,  Hans-Georg Hennemann ,  Harald Haeger ,  Thomas Haas ,  Wilfried Bluemke

IPC分类号： C12P13/00

CPC分类号： C12P13/001 ,  C12N9/0008 ,  C12N9/0016 ,  C12N9/0069 ,  C12N9/1096 ,  C12N9/1288 ,  C12P13/005 ,  Y02P20/582

摘要： The invention provides a whole cell catalyst which expresses a recombinant α-dioxygenase or the combination of a recombinant fatty acid reductase and a phosphopantetheinyl transferase phosphopantetheinylating the fatty acid reductase, and which in addition to the α-dioxygenase and/or the combination of fatty acid reductase and phosphopantetheinyl transferase expresses a transaminase, characterized in that the phosphopantetheinyl transferase and/or transaminase is preferably recombinant; and a method for the conversion of a fatty acid, ω-hydroxy fatty acid, ω-oxo fatty acid or a monoester thereof to an amine, comprising oxidation of the fatty acid, ω-hydroxy fatty acid, ω-oxo fatty acid or the monoester thereof to an oxidation product by contacting with an alkane hydroxylase and/or alcohol dehydrogenase, contacting the oxidation product with a phosphopantetheinylated fatty acid reductase or a α-dioxygenase to give an aldehyde, and contacting the aldehyde with a transaminase.

摘要翻译： 本发明提供一种全细胞催化剂，其表达重组α-加氧酶或重组脂肪酸还原酶与磷酸巯基乙酰转移酶的磷酸聚糖乙酰化脂肪酸还原酶的组合，除了α-加氧酶和/或脂肪酸的组合 还原酶和磷酸泛酰乙酰转移酶表达转氨酶，其特征在于磷酸泛酰乙酰转移酶和/或转氨酶优选是重组的; 以及将脂肪酸ω-羟基脂肪酸，ω-氧代脂肪酸或其单酯转化成胺的方法，包括脂肪酸，ω-羟基脂肪酸，ω-氧代脂肪酸或 其单酯通过与烷烃羟化酶和/或醇脱氢酶接触而与氧化产物接触，使氧化产物与磷酸泛酰乙酰化的脂肪酸还原酶或α-双加氧酶接触，得到醛，并使醛与转氨酶接触。