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    Dnazymes for Inhibition of Japanese Encephalitis Virus Replication
    1.
    发明申请
    Dnazymes for Inhibition of Japanese Encephalitis Virus Replication 审中-公开
    抑制日本脑炎病毒复制的Dnazymes

    公开(公告)号：US20090010907A1

    公开(公告)日：2009-01-08

    申请号：US11721596

    申请日：2005-12-14

    申请人： Sudhanshu Vrati ,  Mohan Babu Appaiahgari

    发明人： Sudhanshu Vrati ,  Mohan Babu Appaiahgari

    IPC分类号： A61K38/43 ,  C12N9/00 ,  A61P31/00 ,  C12P19/34

    CPC分类号： C12N15/1131 ,  C12N2310/12 ,  C12N2310/315 ,  C12N2310/3519 ,  C12N2770/24111

    摘要： The present invention relates to synthetic catalytic DNA molecules or DNAzymes which specifically cleave the RNA sequences of the Japanese Encephalitis Viral genome and is useful in treating Japanese Encephalitis infection. The DNAzyme comprises of a chemical modification, a catalytic domain and two hybridizing arms. The DNAzymes are 29-45 nucleotides in length. The 3′ end of the DNAzyme is tethered to a poly-(G)10 tail (SEQ ID NO: 46) and the molecule comprises of at least one chemical modification. The chemical modifications are in the form of sugar modification, nucleic acid base modification, and/or phosphate backbone modification. The catalytic DNA molecule inhibits JEV replication in vitro in cultured cells and in vivo in the mouse brain. The present invention also relates to the method of treatment of Japanese encephalitis comprising the steps of introducing the catalytic DNA molecule or DNAzyme into the infected cells under conditions suitable for cleavage and reduction of viral titres.

    摘要翻译： 本发明涉及特异性切割日本脑炎病毒基因组的RNA序列的合成催化DNA分子或DNA酶，可用于治疗日本脑炎感染。 DNAzyme由化学修饰，催化结构域和两个杂交臂组成。 DNA酶长度为29-45个核苷酸。 DNA酶的3'端连接到聚（G）10尾（SEQ ID NO：46），并且分子包含至少一种化学修饰。 化学修饰是糖修饰，核酸碱基修饰和/或磷酸酯骨架修饰的形式。 催化DNA分子在培养细胞中体外抑制JEV复制，并在小鼠脑中体内抑制。 本发明还涉及治疗日本脑炎的方法，包括以下步骤：在适合于切割和还原病毒滴度的条件下将催化DNA分子或DNA酶引入感染的细胞中。