公开(公告)号：US20150274769A1

公开(公告)日：2015-10-01

申请号：US14300623

申请日：2014-06-10

申请人： Super Well Biotechnology Corporation ,  Gwo Xi Stem Cell Applied Technology Co., Ltd.

发明人： Zer-Ran YU ,  Be-Jen WANG ,  Shu-Mei LIN ,  Hua-Ching LIN ,  Ming-Hsi CHUANG ,  Chu-Ting LIU ,  Chiu-Ying PENG ,  Lin-Hsiang CHUANG

IPC分类号： C07J1/00

CPC分类号： C07J1/007 ,  A61K35/50 ,  C07J1/0055 ,  Y02P20/544

摘要： The present invention provides a method of separating estrogen from placenta, which uses supercritical fluid technique to load the placenta powder of human body or sheep, pig, deer and other animals into an extraction tank. Under the operating conditions of preset pressure and temperature, supercritical solvent is added into the extraction tank to extract estrogen from placenta, so as to acquire de-estrogen placenta powder and placenta extract liquor. Under the same condition, the de-estrogen placenta extract liquor and supercritical solvent are added by a preset volume flow ratio into an adsorption tank. The estrogen in the placenta extract liquor is adsorbed by the adsorption tank to obtain de-estrogen placenta extract. It is then eluted with ethanol solution by gradient proportion to obtain purified natural estrogen.

摘要翻译： 本发明提供了从胎盘分离雌激素的方法，其使用超临界流体技术将人体胎盘粉或绵羊，猪，鹿等动物加载到提取罐中。 在预设压力和温度的操作条件下，将超临界溶剂加入提取罐中，从胎盘中提取雌激素，以获得雌激素胎盘粉和胎盘提取液。 在相同的条件下，将去雌激素胎盘提取液和超临界溶剂以预设的体积流量比添加到吸附罐中。 胎盘提取液中的雌激素被吸附槽吸附，得到雌激素胎盘提取物。 然后用乙醇溶液以梯度比例洗脱，得到纯化的天然雌激素。

公开(公告)号：US20160159847A1

公开(公告)日：2016-06-09

申请号：US15019795

申请日：2016-02-09

申请人： Super Well Biotechnology Corporation ,  Ultra-Microrigin Biomedical Technology Co., Ltd.

发明人： Zer-Ran YU ,  Be-Jen WANG ,  Shu-Mei LIN ,  Hua-Ching LIN ,  Ming-Hsi CHUANG ,  Chu-Ting LIU ,  Chiu-Ying PENG ,  Lin-Hsiang CHUANG

IPC分类号： C07J1/00 ,  A61K35/50

CPC分类号： C07J1/007 ,  A61K35/50 ,  C07J1/0055 ,  Y02P20/544

摘要： The present invention provides a method of separating estrogen from placenta, which uses supercritical fluid technique to load the placenta powder of human body or sheep, pig, deer and other animals into an extraction tank. Under the operating conditions of preset pressure and temperature, supercritical solvent is added into the extraction tank to extract estrogen from placenta, so as to acquire de-estrogen placenta powder and placenta extract liquor. Under the same condition, the de-estrogen placenta extract liquor and supercritical solvent are added by a preset volume flow ratio into an adsorption tank. The estrogen in the placenta extract liquor is adsorbed by the adsorption tank to obtain de-estrogen placenta extract. It is then eluted with ethanol solution by gradient proportion to obtain purified natural estrogen.

公开(公告)号：US20160074773A1

公开(公告)日：2016-03-17

申请号：US14702388

申请日：2015-05-01

申请人： Super Well Biotechnology Corporation ,  Ultra-Microrigin Biomedical Technology Co., Ltd.

发明人： Zer-Ran YU ,  Be-Jen WANG ,  Shu-Mei LIN ,  Hua-Ching LIN ,  Ming-Hsi CHUANG ,  Chu-Ting LIU ,  I-Lung YU ,  Chiu-Ying PENG ,  Lin-Hsiang CHUANG

IPC分类号： B01D11/02 ,  A61K38/17 ,  A61K38/18 ,  A61K35/50

CPC分类号： B01D11/0203 ,  A61K35/50

摘要： The present invention provides a method to separate and purify functional ingredients in placenta using supercritical fluid technology, where, placenta extract liquid and supercritical CO2 solvent are guided into a fractionation tank constantly in a preset velocity at a preset temperature and pressure to extract peptides, proteins and other functional ingredients. And then, the solution is conveyed through the three fractionation tanks at a preset temperature and depressurized gradually to separate, fractionate and purify the content of placenta peptide and protein, so as to obtain the high purity of peptides, proteins, active factors and other functional ingredients.

摘要翻译： 本发明提供了一种使用超临界流体技术分离和纯化胎盘中的功能成分的方法，其中将胎盘提取液和超临界CO 2溶剂以预设的速度在预设的温度和压力下恒定地引导到分馏罐中以提取肽，蛋白质 和其他功能成分。 然后，将溶液在预设温度下通过三个分级罐输送并逐渐减压，分离，分馏和纯化胎盘肽和蛋白质的含量，以获得高纯度的肽，蛋白质，活性因子和其他功能 配料。