公开(公告)号：US06383488B1

公开(公告)日：2002-05-07

申请号：US09341833

申请日：1999-10-14

申请人： Susana Vazquez Ramudo ,  Guadalupe Guzman Tirado ,  Gerardo Enrique Guillen Nieto ,  Orlando Luis Pardo Lazo ,  Glay Chinea Santiago ,  Ana Beatriz Perez Diaz ,  Maritza Pupo Antunez ,  Rosmari Rodriguez Roche ,  Osvaldo Reyes Acosta ,  Hilda Elisa Garay Perez ,  Gabriel Padron Palomares ,  Maylin Alvarez Vera ,  Luis Morier Diaz ,  Omaida Perez Insuita ,  Jose Luis Pelegrino Martinez De La Cotera

发明人： Susana Vazquez Ramudo ,  Guadalupe Guzman Tirado ,  Gerardo Enrique Guillen Nieto ,  Orlando Luis Pardo Lazo ,  Glay Chinea Santiago ,  Ana Beatriz Perez Diaz ,  Maritza Pupo Antunez ,  Rosmari Rodriguez Roche ,  Osvaldo Reyes Acosta ,  Hilda Elisa Garay Perez ,  Gabriel Padron Palomares ,  Maylin Alvarez Vera ,  Luis Morier Diaz ,  Omaida Perez Insuita ,  Jose Luis Pelegrino Martinez De La Cotera

IPC分类号： A61K3912

CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K39/00 ,  C12N2770/24122 ,  G01N2333/18 ,  Y02A50/386

摘要： The present invention relates to five synthetic peptides of pre-M/M protein of Dengue-2 virus, corresponding to amino acid sequences 3-31, 45-67, 57-92, 69-93 and 103-124. The anti-peptide immune response was evaluated in mice. Recombinant fusion proteins were also obtained, including regions of pre-M/M protein. The presence of B cell epitopes in both mice and humans was demonstrated in the pre-M/M protein peptides. Peptides 3-31 and 103-124 elicited neutralizing antibodies against the four serotypes of Dengue virus. Virus-specific proliferative responses were demonstrated in mice immunized with non-conjugated peptides 3-31 and 57-92. Mice immunized with conjugated peptides 3-31, 57-92, and 69-93 were protected when they were challenged with Dengue-2 virus. Thus, the presence of sequential epitopes in Pre-M/M protein of Dengue-2 virus was demonstrated, as well as their relevance in the immune response against this flavivirus.

摘要翻译： 本发明涉及对应于氨基酸序列3-31,45-67,57-92,69-93和103-124的五种登革热-2病毒前M / M蛋白的合成肽。 在小鼠中评估抗肽免疫应答。 还获得了重组融合蛋白，包括前M / M蛋白的区域。 在M-M蛋白肽前体中证实了小鼠和人体中B细胞表位的存在。 肽3-31和103-124引发针对登革热病毒的四种血清型的中和抗体。 在用非结合肽3-31和57-92免疫的小鼠中证明病毒特异性增殖应答。 当用登革热-2病毒攻击时，用缀合肽3-31,57-92和69-93免疫的小鼠被保护。 因此，证实了登革2型病毒的Pre-M / M蛋白中序列表位的存在，以及它们在针对该黄病毒的免疫应答中的相关性。