公开(公告)号：US06558923B2

公开(公告)日：2003-05-06

申请号：US09988010

申请日：2001-11-16

申请人： Sylvie Paulous ,  Pierre Charneau ,  Véronique Zennou ,  François Clavel ,  Esther Race ,  Elisabeth Dam ,  Véronique Obry

发明人： Sylvie Paulous ,  Pierre Charneau ,  Véronique Zennou ,  François Clavel ,  Esther Race ,  Elisabeth Dam ,  Véronique Obry

IPC分类号： C12P2106

CPC分类号： C12Q1/703 ,  C12Q1/70

摘要： The invention provides an in vitro single cycle, recombinant virus assay (RVA) for determining inhibition of HIV replication by a protease inhibitor. The assay comprises transfecting a human epithelial cell line with amplified HIV protease sequences of an HIV virus; an HIV envelope defective molecular clone having a complete deletion of its protease coding sequence as well as two separate deletions in its env gene and a deletion of part of its gag gene; and a plasmid containing HIV envelope coding sequence under the control of a promoter for phenotypic complementation of the envelope defective molecular clone. The transfected cells are cultured in the presence of a protease inhibitor to produce a testable stock of infectious particles that can be used to infect indicator cells containing an indicator gene without amplification of the infectious particles prior to infecting the indicator cells. Accumulation of indicator gene product is a measure of inhibition of HIV replication by the protease inhibitor.

公开(公告)号：US20070269797A9

公开(公告)日：2007-11-22

申请号：US10435659

申请日：2003-05-12

申请人： Francois Clavel ,  Fabrizio Mammano ,  Esther Race ,  Elisabeth Dam ,  Veronique Obry ,  Virginie Trouplin

发明人： Francois Clavel ,  Fabrizio Mammano ,  Esther Race ,  Elisabeth Dam ,  Veronique Obry ,  Virginie Trouplin

IPC分类号： C12Q1/70

CPC分类号： C12Q1/703

摘要： The present invention relates to a method for analyzing the phenotypic characteristics shown by certain virus strains, particularly human immunodeficiency viruses, involving the construction of a recombinant virus obtained by homologous recombination. The present invention also relates a kit comprising the primers, vectors, cell hosts, products and reagents required to carry out PCR amplification, and the products and reagents used to detect a marker, for the implementation of the method according to the invention.

摘要翻译： 本发明涉及一种用于分析特定病毒株，特别是人类免疫缺陷病毒所表现的表型特征的方法，涉及构建通过同源重组获得的重组病毒。 本发明还涉及包含引物，载体，细胞宿主，进行PCR扩增所需的产物和试剂的试剂盒，以及用于检测标记物的产品和试剂，用于实施根据本发明的方法。