公开(公告)号：US20070269797A9

公开(公告)日：2007-11-22

申请号：US10435659

申请日：2003-05-12

Applicant: Francois Clavel ,  Fabrizio Mammano ,  Esther Race ,  Elisabeth Dam ,  Veronique Obry ,  Virginie Trouplin

Inventor： Francois Clavel ,  Fabrizio Mammano ,  Esther Race ,  Elisabeth Dam ,  Veronique Obry ,  Virginie Trouplin

IPC: C12Q1/70

CPC classification number: C12Q1/703

Abstract: The present invention relates to a method for analyzing the phenotypic characteristics shown by certain virus strains, particularly human immunodeficiency viruses, involving the construction of a recombinant virus obtained by homologous recombination. The present invention also relates a kit comprising the primers, vectors, cell hosts, products and reagents required to carry out PCR amplification, and the products and reagents used to detect a marker, for the implementation of the method according to the invention.

Abstract translation: 本发明涉及一种用于分析特定病毒株，特别是人类免疫缺陷病毒所表现的表型特征的方法，涉及构建通过同源重组获得的重组病毒。 本发明还涉及包含引物，载体，细胞宿主，进行PCR扩增所需的产物和试剂的试剂盒，以及用于检测标记物的产品和试剂，用于实施根据本发明的方法。

公开(公告)号：US06979535B2

公开(公告)日：2005-12-27

申请号：US10133357

申请日：2002-04-29

Applicant: Marc Alizon ,  Luc Montagnier ,  Denise Guetard ,  Francois Clavel ,  Pierre Sonigo ,  Mireille Guyader

Inventor： Marc Alizon ,  Luc Montagnier ,  Denise Guetard ,  Francois Clavel ,  Pierre Sonigo ,  Mireille Guyader

IPC: A61K38/00 ,  A61K39/00 ,  C07K7/06 ,  C07K14/16 ,  C12N7/00 ,  C12Q1/70 ,  G01N33/569 ,  A61K39/21

CPC classification number: C07K7/06 ,  A61K38/00 ,  A61K39/00 ,  C07K14/005 ,  C12N7/00 ,  C12N2740/16021 ,  C12N2740/16122 ,  C12N2740/16222 ,  C12Q1/703 ,  G01N33/56988 ,  G01N2333/162 ,  G01N2469/20

Abstract: A method for diagnosing an HIV-2 (LAV-II) infection and a kit containing reagents for the same is disclosed. These reagents include cDNA probes which are capable of hybridizing to at least a portion of the genome of HIV-2. In one embodiment, the DNA probes are capable of hybridizing to the entire genome of HIV-2. These reagents also include polypeptides encoded by some of these DNA sequences.

Abstract translation: 公开了用于诊断HIV-2（LAV-II）感染的方法和用于其的试剂盒。 这些试剂包括能够与HIV-2的至少一部分基因组杂交的cDNA探针。 在一个实施方案中，DNA探针能够与HIV-2的整个基因组杂交。 这些试剂还包括由这些DNA序列中的一些编码的多肽。

公开(公告)号：US06355789B1

公开(公告)日：2002-03-12

申请号：US08468424

申请日：1995-06-06

Applicant: Marc Alizon ,  Luc Montagnier ,  Denise Geutard ,  Francois Clavel ,  Pierre Sonigo ,  Mireille Guyader

Inventor： Marc Alizon ,  Luc Montagnier ,  Denise Geutard ,  Francois Clavel ,  Pierre Sonigo ,  Mireille Guyader

IPC: C07H2104

CPC classification number: C07K7/06 ,  A01K2217/05 ,  A01K2267/01 ,  A61K38/00 ,  A61K39/00 ,  C07K14/005 ,  C07K14/555 ,  C12N7/00 ,  C12N15/8509 ,  C12N15/90 ,  C12N2730/10122 ,  C12N2740/16021 ,  C12N2740/16122 ,  C12N2740/16222 ,  C12N2740/16322 ,  C12N2770/32622 ,  C12Q1/703 ,  G01N33/56988 ,  G01N2333/162 ,  G01N2469/20 ,  Y02A50/466 ,  Y10S435/974

Abstract: The present invention is directed toward nucleic acids containing the full-length human immunodeficiency virus type 2 ROD (HIV-2ROD) pol gene. HIV-2, which was originally designated lymphadenopathy-associated virus type II (LAV-II), was isolated from AIDS patients in West Africa. The virus is genotypically and phenotypically distinct from HIV-1 and bears a closer genetic relationship to the simian immunodeficiency virus (SIV). The present invention describes the preparation of HIV-2ROD proviral molecular clones from a genomic lambda phage library of CD4+-infected cells. The complete nucleotide sequence of the full-length genome was determined and the putative gag, pol, env, vif (Q), vpr (R), vpx (X), nef (F), tat, and rev (art) genes identified. The claimed invention is directed toward nucleic acids containing the full-length HIV-2ROD pol gene (nt 1829-4936). These nucleic acids should prove useful as diagnostic reagents for the detection of HIV-2 and facilitate expression of the pol gene product.

Abstract translation: 本发明涉及含有全长人类免疫缺陷病毒2型ROD（HIV-2ROD）pol基因的核酸。 HIV-2，最初被指定为淋巴结病相关病毒II型（LAV-II），从西非艾滋病患者中分离出来。 该病毒在基因型和表型上与HIV-1不同，并与猿猴免疫缺陷病毒（SIV）具有更密切的遗传关系。 本发明描述了来自CD4 +感染细胞的基因组λ噬菌体文库的HIV-2ROD原病毒分子克隆的制备。 确定全长基因组的完整核苷酸序列，并确定推定的gag，pol，env，vif（Q），vpr（R），vpx（X），nef（F），tat和rev 。 要求保护的发明涉及含有全长HIV-2ROD pol基因（nt 1829-4936）的核酸。 这些核酸应被证明可用作检测HIV-2的诊断试剂，并有助于pol基因产物的表达。

公开(公告)号：US5770703A

公开(公告)日：1998-06-23

申请号：US468774

申请日：1995-06-06

Applicant: Marc Alizon ,  Luc Montagnier ,  Denise Geutard ,  Francois Clavel ,  Pierre Sonigo ,  Mireille Guyader

Inventor： Marc Alizon ,  Luc Montagnier ,  Denise Geutard ,  Francois Clavel ,  Pierre Sonigo ,  Mireille Guyader

IPC: A61K38/00 ,  A61K39/00 ,  A61P31/18 ,  C07K7/06 ,  C07K14/02 ,  C07K14/105 ,  C07K14/16 ,  C07K14/555 ,  C12N7/00 ,  C12N15/85 ,  C12N15/90 ,  C12Q1/70 ,  G01N33/569 ,  C07K14/155 ,  A61K39/21 ,  C07H21/04

CPC classification number: C07K7/06 ,  C07K14/005 ,  C07K14/555 ,  C12N15/8509 ,  C12N15/90 ,  C12N7/00 ,  C12Q1/703 ,  G01N33/56988 ,  A01K2217/05 ,  A01K2267/01 ,  A61K38/00 ,  A61K39/00 ,  C12N2730/10122 ,  C12N2740/16021 ,  C12N2740/16122 ,  C12N2740/16222 ,  C12N2740/16322 ,  C12N2770/32622 ,  G01N2333/162 ,  G01N2469/20 ,  Y10S435/974

Abstract: The inventors disclose nucleic acids of HIV-2 encoding the entire envelope glycoprotein and peptide regions thereof. Also described are purified and cloned nucleic acids having the entire HIV-2 genome. The nucleic acids can be used to produce polypeptides corresponding to the HIV-2 envelope gene or peptides from specific regions of the HIV-2 envelope gene. Uses in hybridization assays are also disclosed.

Abstract translation: 本发明人公开了编码整个包膜糖蛋白及其肽区的HIV-2的核酸。 还描述了具有整个HIV-2基因组的纯化和克隆的核酸。 核酸可用于产生对应于HIV-2包膜基因的多肽或来自HIV-2包膜基因的特定区域的肽。 还公开了杂交测定中的用途。

公开(公告)号：US5030718A

公开(公告)日：1991-07-09

申请号：US462984

申请日：1990-01-10

Applicant: Luc Montagnier ,  Denise Guetard ,  Francoise Brun-Vezinet ,  Francois Clavel

Inventor： Luc Montagnier ,  Denise Guetard ,  Francoise Brun-Vezinet ,  Francois Clavel

IPC: A61K38/00 ,  A61K39/00 ,  C07K7/06 ,  C07K14/16 ,  C12N7/00 ,  C12Q1/70 ,  G01N33/569

CPC classification number: C07K14/005 ,  C07K7/06 ,  C12N7/00 ,  C12Q1/703 ,  G01N33/56988 ,  A61K38/00 ,  A61K39/00 ,  C12N2740/16021 ,  C12N2740/16122 ,  C12N2740/16222 ,  G01N2333/162 ,  G01N2469/20 ,  Y10S435/974 ,  Y10S435/975 ,  Y10S530/826 ,  Y10S930/221

Abstract: The invention relates to a new variety of retroviruses designated Human Immunodeficiency virus Type II, HIV-II, samples of which have been deposited at CNCM as I-502 and I-532. It also concerns purified forms of the antigens which can be obtained from this virus, in particular from the gp 36 and gp 130-140 proteins. These various antigens are useful in medical diagnosis and kits, in particular by being placed in contact with serum of the patient to be diagnosed. Lastly, the invention relates to immunizing compositions, in particular containing at least one of glycoproteins gp 36 and gp 130-140.

公开(公告)号：US20080261197A1

公开(公告)日：2008-10-23

申请号：US11591678

申请日：2006-11-02

Applicant: Pierre Charneau ,  Francois Clavel ,  Andrew Borman ,  Caroline Quillent ,  Denise Guetard ,  Luc Montagnier ,  Jacqueline Donjon De Saint-Martin ,  Jacques H.M. Cohen

Inventor： Pierre Charneau ,  Francois Clavel ,  Andrew Borman ,  Caroline Quillent ,  Denise Guetard ,  Luc Montagnier ,  Jacqueline Donjon De Saint-Martin ,  Jacques H.M. Cohen

IPC: C12Q1/70 ,  C07K14/16 ,  C07K5/06 ,  C07K5/08 ,  C07K5/10 ,  C07K16/10 ,  C12N1/00 ,  C12N1/20 ,  C12N7/00 ,  C12N15/63 ,  C07K7/06 ,  C07K7/08 ,  C07K14/00 ,  C07H21/00

CPC classification number: C07K14/005 ,  C12N2740/16021 ,  C12N2740/16022 ,  C12N2740/16043 ,  C12N2740/16122 ,  C12N2740/16222 ,  C12N2740/16322 ,  G01N33/56988 ,  Y10S435/975

Abstract: An HIV-1 type (or subtype) O retrovirus protein, or a natural or synthetic polypeptide or peptide including at least a part of said protein, which is capable of being recognised by antibodies isolated from a serum resulting from infection by an HIV-1 type O VAU strain or an HIV-1 type (or subtype) O DUR strain.

Abstract translation: HIV-1型（或亚型）O逆转录病毒蛋白，或包含至少一部分所述蛋白质的天然或合成多肽或肽，其能够被从HIV-1感染引起的血清分离的抗体识别 O型VAU菌株或HIV-1型（或亚型）O DUR菌株。

公开(公告)号：US06514691B1

公开(公告)日：2003-02-04

申请号：US09191384

申请日：1998-11-13

Applicant: Marc Alizon ,  Luc Montagnier ,  Denise Guetard ,  Francois Clavel ,  Pierre Sonigo ,  Mireille Guyader

Inventor： Marc Alizon ,  Luc Montagnier ,  Denise Guetard ,  Francois Clavel ,  Pierre Sonigo ,  Mireille Guyader

IPC: C12Q170

CPC classification number: C07K7/06 ,  A61K38/00 ,  A61K39/00 ,  C07K14/005 ,  C12N7/00 ,  C12N2740/16021 ,  C12N2740/16122 ,  C12N2740/16222 ,  C12Q1/703 ,  G01N33/56988 ,  G01N2333/162 ,  G01N2469/20

Abstract: This invention is directed toward the isolation of a novel retrovirus, the human immune deficiency virus type 2 (HIV-2, previously named LAV-2), from patients with acquired immune deficiency syndrome (AIDS) originating from West Africa. This virus is related to HIV-1, the causative agent of AIDS, both by its morphology and by its tropism and in vitro cytopathic effect on CD4 (T4) positive cell lines and lymphocytes. However, preliminary hybridization experiments indicated that there are substantiated differences between the sequences of the two genomes. Furthermore, the proteins of HIV-1 and HIV-2 have different sizes and their serological cross-reactivity is restricted to the major core protein, as the envelope glycoproteins of HIV-2 are not immunoprecipitated by HIV-1 positive sera. Overlapping molecular clones were obtained and the complete nucleotide sequence of the gag and env genes was ascertained. An antigenic envelope polypeptide having the following amino acid sequence was identified: NH2-VTAIEKYLEDQARLNSWGCAFRQVC-COOH. The peptide is useful for the detection of HIV-2-specific immunological reagents.

Abstract translation: 本发明针对来自西非的获得性免疫缺陷综合征（AIDS）患者的新型逆转录病毒，2型免疫缺陷病毒（HIV-2，以前称为LAV-2））的分离。 这种病毒与HIV-1（艾滋病的致病因子）有关，其形态以及其对CD4（T4）阳性细胞系和淋巴细胞的向性和体外细胞病变作用。 然而，初步杂交实验表明，两个基因组的序列之间存在证实的差异。 此外，HIV-1和HIV-2的蛋白质具有不同的大小，并且其血清学交叉反应性限于主要核心蛋白质，因为HIV-2的包膜糖蛋白不被HIV-1阳性血清免疫沉淀。 获得重叠的分子克隆，并确定了gag和env基因的完整核苷酸序列。 鉴定了具有以下氨基酸序列的抗原性包膜多肽：NH2-VTAIEKYLEDQA​​RLNSWGCAFRQVC-COOH。 该肽可用于检测HIV-2特异性免疫试剂。

公开(公告)号：US06162439A

公开(公告)日：2000-12-19

申请号：US466707

申请日：1995-06-06

Applicant: Marc Alizon ,  Luc Montagnier ,  Denise Guetard ,  Francois Clavel ,  Pierre Sonigo ,  Mireille Guyader

Inventor： Marc Alizon ,  Luc Montagnier ,  Denise Guetard ,  Francois Clavel ,  Pierre Sonigo ,  Mireille Guyader

IPC: A61K38/00 ,  A61K39/00 ,  A61P31/18 ,  C07K7/06 ,  C07K14/02 ,  C07K14/105 ,  C07K14/16 ,  C07K14/555 ,  C12N7/00 ,  C12N15/85 ,  C12N15/90 ,  C12Q1/70 ,  G01N33/569 ,  A61K39/21

CPC classification number: C07K7/06 ,  A01K2217/05 ,  A01K2267/01 ,  A61K38/00 ,  A61K39/00 ,  C07K14/005 ,  C07K14/555 ,  C12N7/00 ,  C12N15/8509 ,  C12N15/90 ,  C12N2730/10122 ,  C12N2740/16021 ,  C12N2740/16122 ,  C12N2740/16222 ,  C12N2740/16322 ,  C12N2770/32622 ,  C12Q1/703 ,  G01N33/56988 ,  G01N2333/162 ,  G01N2469/20 ,  Y02A50/466 ,  Y10S435/974

Abstract: This invention is directed toward polypeptides derived from novel lentiviruses. A novel lentivirus, designated the human immunodeficiency virus type 2, was isolated from West African patients with acquired immune deficiency syndrome (AIDS). Several isolates were obtained and designated HIV-2.sub.ROD, HIV-2.sub.IRMO, and HIV-2.sub.EHO. A recombinant lambda phage library was constructed from HIV-2.sub.ROD -infected CEM genomic DNA. Overlapping molecular clones were obtained and the nucleotide sequence of the complete 9.5-kilobase (kb) HIV-2.sub.ROD genome ascertained. The genetic organization of HIV-2 is analogous to that of other retroviruses and consists of the 5'LTR-gag-pol-central region-env-nef-3'LTR. The central region also encodes for the regulatory proteins Tat and Rev, as well as the ancillary proteins Vif, Vpr, and Vpx. The proteins encoded by this proviral clone will provide novel immunologic, biochemic, and diagnostic reagents useful for the detection of HIV-2.

Abstract translation: 本发明涉及来源于新型慢病毒的多肽。 从西非获得性免疫缺陷综合征（AIDS）患者中分离出一种称为2型人类免疫缺陷病毒的新型慢病毒。 获得了几种分离物，并命名为HIV-2ROD，HIV-2IRMO和HIV-2EHO。 从HIV-2ROD感染的CEM基因组DNA构建重组λ噬菌体文库。 获得重叠的分子克隆，并确定了完整的9.5K碱基（kb）HIV-2ROD基因组的核苷酸序列。 HIV-2的遗传组织类似于其他逆转录病毒，并由5'LTR-gag-pol-中心区域-nv-nef-3'LTR组成。 中心区也编码调节蛋白Tat和Rev，以及辅助蛋白Vif，Vpr和Vpx。 由该前病毒克隆编码的蛋白质将提供用于检测HIV-2的新型免疫学，生物化学和诊断试剂。

公开(公告)号：US6054565A

公开(公告)日：2000-04-25

申请号：US234875

申请日：1994-04-28

Applicant: Marc Alizon ,  Luc Montagnier ,  Denise Geutard ,  Francois Clavel ,  Pierre Sonigo ,  Mireille Guyader

Inventor： Marc Alizon ,  Luc Montagnier ,  Denise Geutard ,  Francois Clavel ,  Pierre Sonigo ,  Mireille Guyader

IPC: A61K38/00 ,  A61K39/00 ,  C07K7/06 ,  C07K14/16 ,  C12N7/00 ,  C12Q1/70 ,  G01N33/569 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/68

CPC classification number: C07K7/06 ,  C07K14/005 ,  C12N7/00 ,  C12Q1/703 ,  G01N33/56988 ,  A61K38/00 ,  A61K39/00 ,  C12N2740/15022 ,  C12N2740/16021 ,  C12N2740/16122 ,  C12N2740/16222 ,  C12N2740/16322 ,  G01N2333/162 ,  G01N2469/20 ,  Y10S435/974 ,  Y10S435/975 ,  Y10S530/81

Abstract: A method for diagnosing an HIV-2 (LAV-II) infection and a kit containing reagents for the same is disclosed. These reagents include cDNA probes which are capable of hybridizing to at least a portion of the genome of HIV-2. In the embodiment, the DNA probes are capable of hybridizing to the entire genome of HIV-2. These reagents also include polypeptides encoded by some of these DNA sequences.

Abstract translation: 公开了用于诊断HIV-2（LAV-II）感染的方法和用于其的试剂盒。 这些试剂包括能够与HIV-2的至少一部分基因组杂交的cDNA探针。 在该实施方案中，DNA探针能够与HIV-2的整个基因组杂交。 这些试剂还包括由这些DNA序列中的一些编码的多肽。

公开(公告)号：US5866319A

公开(公告)日：1999-02-02

申请号：US468093

申请日：1995-06-06

Applicant: Marc Alizon ,  Luc Montagnier ,  Denise Guetard ,  Francois Clavel ,  Pierre Sonigo ,  Mireille Guyader

Inventor： Marc Alizon ,  Luc Montagnier ,  Denise Guetard ,  Francois Clavel ,  Pierre Sonigo ,  Mireille Guyader

IPC: A61K38/00 ,  A61K39/00 ,  C07K7/06 ,  C07K14/16 ,  C12N7/00 ,  C12Q1/70 ,  G01N33/569

CPC classification number: C07K14/005 ,  C07K7/06 ,  C12N7/00 ,  C12Q1/703 ,  G01N33/56988 ,  A61K38/00 ,  A61K39/00 ,  C12N2740/16021 ,  C12N2740/16122 ,  C12N2740/16222 ,  G01N2333/162 ,  G01N2469/20 ,  Y10S435/974 ,  Y10S435/975 ,  Y10S530/826 ,  Y10S930/221

Abstract: A method for diagnosing an HIV-2 (LAV-II) infection and a kit containing reagents for the same is disclosed. These reagents include cDNA probes which are capable of hybridizing to at least a portion of the genome of HIV-2. In one embodiment, the DNA probes are capable of hybridizing to the entire genome of HIV-2. These reagents also include polypeptides encoded by some of these DNA sequences.

Abstract translation: 公开了用于诊断HIV-2（LAV-II）感染的方法和用于其的试剂盒。 这些试剂包括能够与HIV-2的至少一部分基因组杂交的cDNA探针。 在一个实施方案中，DNA探针能够与HIV-2的整个基因组杂交。 这些试剂还包括由这些DNA序列中的一些编码的多肽。