公开(公告)号：US09359622B2

公开(公告)日：2016-06-07

申请号：US13954957

申请日：2013-07-30

Applicant: Symrise AG

Inventor： Jens Michael Hilmer ,  Egon Gross ,  Gerhard Krammer ,  Jakob Peter Ley ,  Mechthild Gall ,  Uwe Bornscheuer ,  Maren Thomsen ,  Christin Peters ,  Patrick Jonczyk ,  Sascha Beutel ,  Thomas Scheper

IPC: C12P7/26 ,  C12N1/21 ,  C12N9/02 ,  C12N9/90 ,  C12N15/53 ,  C12N15/61 ,  C12N15/63 ,  C12R1/01 ,  C12R1/19 ,  C12R1/645 ,  C12R1/865 ,  C12R1/84

CPC classification number: C12P7/26 ,  C12N9/001 ,  C12N9/90 ,  C12R1/01 ,  C12R1/19 ,  C12R1/645 ,  C12R1/84 ,  C12R1/865 ,  C12Y103/01031 ,  C12Y505/01006 ,  Y02P20/52

Abstract: A method for production of a dihydrochalcone, especially of phloretin, using a transgenic microorganism, containing a nucleic acid section (a), comprising or consisting of a gene coding for a bacterial chalcone isomerase, and/or a nucleic acid section (a′), comprising or consisting of a gene coding for a plant chalcone isomerase, and a nucleic acid section (b), comprising or consisting of a gene coding for a bacterial enoate reductase, corresponding transgenic microorganisms, containing a nucleic acid section (a), comprising or consisting of a gene coding for a bacterial chalcone isomerase, and/or a nucleic acid section (a′), comprising or consisting of a gene coding for a plant chalcone isomerase, and/or a nucleic acid section (b), comprising or consisting of a gene coding for a bacterial enoate reductase, and host cells, containing one or more identical or different such vectors.

Abstract translation: 使用含有核酸部分（a）的转基因微生物来制造二氢查耳酮，特别是花旗素的二氢查耳酮的方法，其包含编码细菌查耳酮异构酶的基因和/或核酸部分（a'），或由编码细菌查耳酮异构酶的基因组成。 包含编码植物查耳酮异构酶的基因或核酸部分（b），其包含编码细菌酸酸酯还原酶的基因或由其组成，所述基因含有核酸部分（a），相应的转基因微生物，其包含 或由编码细菌查耳酮异构酶的基因和/或由编码植物查尔酮异构酶的基因组成或由编码植物查耳酮异构酶的核酸部分（a'）组成的核酸部分（a'），和/或核酸部分（b） 由编码细菌酸酯还原酶的基因和含有一个或多个相同或不同的这种载体的宿主细胞组成。

公开(公告)号：US20140045233A1

公开(公告)日：2014-02-13

申请号：US13954957

申请日：2013-07-30

Applicant: Symrise AG

Inventor： Jens Michael Hilmer ,  Egon Gross ,  Gerhard Krammer ,  Jakob Peter Ley ,  Mechthild Gall ,  Uwe Bornscheuer ,  Maren Thomsen ,  Christin Peters ,  Patrick Jonczyk ,  Sascha Beutel ,  Thomas Scheper

IPC: C12P7/26 ,  C12R1/84 ,  C12R1/645 ,  C12R1/865 ,  C12R1/01 ,  C12R1/19

CPC classification number: C12P7/26 ,  C12N9/001 ,  C12N9/90 ,  C12R1/01 ,  C12R1/19 ,  C12R1/645 ,  C12R1/84 ,  C12R1/865 ,  C12Y103/01031 ,  C12Y505/01006 ,  Y02P20/52

Abstract: A method for production of a dihydrochalcone, especially of phloretin, using a transgenic microorganism, containing a nucleic acid section (a), comprising or consisting of a gene coding for a bacterial chalcone isomerase, and/or a nucleic acid section (a′), comprising or consisting of a gene coding for a plant chalcone isomerase, and a nucleic acid section (b), comprising or consisting of a gene coding for a bacterial enoate reductase, corresponding transgenic microorganisms, containing a nucleic acid section (a), comprising or consisting of a gene coding for a bacterial chalcone isomerase, and/or a nucleic acid section (a′), comprising or consisting of a gene coding for a plant chalcone isomerase, and/or a nucleic acid section (b), comprising or consisting of a gene coding for a bacterial enoate reductase, and host cells, containing one or more identical or different such vectors.

Abstract translation: 使用含有核酸部分（a）的转基因微生物来制造二氢查耳酮，特别是花旗素的二氢查耳酮的方法，其包含编码细菌查耳酮异构酶的基因和/或核酸部分（a'），或由编码细菌查耳酮异构酶的基因组成。 包含编码植物查耳酮异构酶的基因或核酸部分（b），其包含编码细菌酸酸酯还原酶的基因或由其组成，所述基因含有核酸部分（a），相应的转基因微生物，其包含 或由编码细菌查耳酮异构酶的基因和/或由编码植物查尔酮异构酶的基因组成或由编码植物查耳酮异构酶的核酸部分（a'）组成的核酸部分（a'），和/或核酸部分（b） 由编码细菌酸酯还原酶的基因和含有一个或多个相同或不同的这种载体的宿主细胞组成。